The purpose of the study was to evaluate multixenobiotic resistance mechanism expression as a biomarker for contaminant exposure in freshwater fishes. Exposure to a known mammalian inhibitor (verapamil, 10 μM) and inducer (rhodamine 123, 3 μM) on the transport protein P-glycoprotein (Pgp) was investigated in the liver of the western mosquitofish and bluegill sunfish. No differences in the activity or expression of Pgp were measured in either species using a fluorometric accumulation assay and western blot analyses. The preliminary results from this study indicate that this detoxification mechanism may not be a sensitive indicator of contaminant exposure in certain teleost species.
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The authors wish to thank Matthew Overturf for his assistance in field collections and experimentation and Nancy Baer for her editorial comments.
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Damaré, C.L., Kaddoumi, A.K. & Baer, K.N. Investigation of the Multixenobiotic Resistance Mechanism in the Freshwater Fishes Western Mosquitofish, Gambusia affinis, and Bluegill Sunfish, Lepomis macrochirus . Bull Environ Contam Toxicol 83, 640 (2009). https://doi.org/10.1007/s00128-009-9846-x
- Bluegill sunfish