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Diazinon Determination Using High Performance Liquid Chromatography: A Comparison of the ENVI-Carb Column with the Immunoaffinity Column for the Pretreatment of Water and Soil Samples


An immunoaffinity chromatography column was developed to remove diazinon from water and soil samples. In this paper, two types of absorbent columns, the immunoaffinity chromatography column and the ENVI-Carb column, were compared. To accomplish this, each of these columns was used to treat water and soil samples that had been spiked with diazinon at concentrations of 2.5 or 5 ng/mL (or ng/g). High performance liquid chromatography was then used to analyze the treated samples. The ENVI-Carb column recovered 87.99%–95.95% of the diazinon from water and soil with CVs of 5.08%–8.06%. The recoveries observed when the immunoaffinity chromatography column was used were slightly lower (52.61%–81.58%); however, it effectively clean up the soil samples.

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This work was supported by the Chinese National “863” High-Tech Research Program (2006AA10Z450).

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Correspondence to Yitong Lu.

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Tang, J., Zhang, M., Cheng, G. et al. Diazinon Determination Using High Performance Liquid Chromatography: A Comparison of the ENVI-Carb Column with the Immunoaffinity Column for the Pretreatment of Water and Soil Samples. Bull Environ Contam Toxicol 83, 626 (2009).

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  • Immunoaffinity
  • Solid phase extraction
  • Diazinon
  • Comparison