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Genome-Wide Gene Expression Profiling in γ-Irradiated Green Alga, Pseudokirchneriella subcapitata, by HiCEP

Abstract

Transcriptome was analyzed in γ-irradiated green alga Pseudokirchneriella subcapitata by high coverage gene expression profiling (HiCEP). Approximately 7,800 expressed genes were detected. Expression levels of 623–707 genes were affected at 100–300 Gy. Nucleotide sequences of 41 up-regulated genes were determined. The quantitative reverse transcription polymerase chain reaction validated the up-regulation. Two genes had homology to genes related to ionizing radiation. These results indicate usefulness of HiCEP for screening of stress-responsive genes in species that are ecotoxicologically important but for which genomic sequence information is lacking.

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Acknowledgments

Gene expression profiling in this study was performed by the HiCEP unit team founded under the NIRS president’s initiative. The authors thank Syunsuke Ando, Sachi Inukai, Toshitaro Takata and Atsuko Matsumoto (NIRS) for their technical support.

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Correspondence to Shoichi Fuma.

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Fuma, S., Nakamori, T., Ishii, N. et al. Genome-Wide Gene Expression Profiling in γ-Irradiated Green Alga, Pseudokirchneriella subcapitata, by HiCEP. Bull Environ Contam Toxicol 83, 301–306 (2009). https://doi.org/10.1007/s00128-009-9780-y

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Keywords

  • Pseudokirchneriella subcapitata
  • Transcriptome
  • HiCEP
  • Ionizing radiation