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Isolation and Simultaneous LC Analysis of Thiram and Its Less Toxic Transformation Product in DS Formulation

Abstract

A simple and sensitive high pressure liquid chromatographic method has been developed for the simultaneous determination of Thiram and its transformation product using isocratic mixture of methanol–water (65:35) at flow rates of 0.75 mL min−1, PDA detector using UV absorbance (λmax) at 217 nm for Thiram and 265.5 nm for the transformation product. The transformation product was isolated from the commercial DS formulation and has been tentatively assigned the structure by 1H NMR and ESI-MS spectral data. The separation is dependent on the nature of the mobile phase, its flow rate and the nature of the HPLC column. The detection limit (signal/noise; S/N = 3) for both Thiram and its transformation product was 0.2 ppm. The method has been successfully applied to analysis of soil and soybean samples spiked with Thiram, its transformation product and a commercial sample containing these products.

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Acknowledgments

Authors are thankful to Indian Council of Agricultural Research (ICAR) for financial assistance under Emeritus Scientist Scheme, and to the Head, Division of Agricultural Chemicals, IARI, New Delhi for providing necessary facilities.

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Correspondence to Suresh Walia.

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Walia, S., Sharma, R.K. & Parmar, B.S. Isolation and Simultaneous LC Analysis of Thiram and Its Less Toxic Transformation Product in DS Formulation. Bull Environ Contam Toxicol 83, 363–368 (2009). https://doi.org/10.1007/s00128-009-9754-0

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Keywords

  • HPLC
  • Thiram
  • DS formulation
  • Transformation product