Skip to main content

Isolation and Simultaneous LC Analysis of Thiram and Its Less Toxic Transformation Product in DS Formulation


A simple and sensitive high pressure liquid chromatographic method has been developed for the simultaneous determination of Thiram and its transformation product using isocratic mixture of methanol–water (65:35) at flow rates of 0.75 mL min−1, PDA detector using UV absorbance (λmax) at 217 nm for Thiram and 265.5 nm for the transformation product. The transformation product was isolated from the commercial DS formulation and has been tentatively assigned the structure by 1H NMR and ESI-MS spectral data. The separation is dependent on the nature of the mobile phase, its flow rate and the nature of the HPLC column. The detection limit (signal/noise; S/N = 3) for both Thiram and its transformation product was 0.2 ppm. The method has been successfully applied to analysis of soil and soybean samples spiked with Thiram, its transformation product and a commercial sample containing these products.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4


  1. Agarwal M, Walia S, Dhingra S, Khambay BPS (2001) Insect growth inhibition, antifeedant and antifungal activity of compounds isolated/derived from Zingiber officinale Roscoe (ginger) rhizomes. Pest Manag Sci 57:289–300

    Article  CAS  Google Scholar 

  2. Anonymous (1991) International Agency for Research on Cancer (IARC)—Summaries & Evaluations. 53:403

  3. Aulakh JS, Sharma VK, Mahajan RK, Malik AK (2004) HPLC-UV determination of tetramethylthiuram disulphide (Thiram) and thiourea in wheat grains and in a commercial sample. EJEAF Che 3(5):751–757

    Google Scholar 

  4. del Silva MP, Procopio JR, Hernandez L (1999) Electrochemical detection in the determination of several dithiocarbamates by reverse-phase liquid chromatography. J Liq Chromatogr Relat Technol 22:463–475

    Article  Google Scholar 

  5. Friedrichs K, Winkeler HD, Gerhards P (1995) Determination of dithiocarbamate residues in food stuff by headstuff gas chromatographiy and flame photometric detection. Z Lebensm Unters Forsch 201:69–73

    Article  CAS  Google Scholar 

  6. Harrington P, Horner J, Hird S, Griffiths T, Reynolds SL (1998) Modification of the method for measurement of dithiocarbamate residues as carbon disulfide in fruit and vegetables. Central Science Laboratory, York UK, p 1

    Google Scholar 

  7. Hill ARC (1992) Headspace methods for dithiocarbamates. In: Cairns T, Sherma J (eds) Modern methods for pesticide analysis. Emerging strategies for pesticide analysis. CRC Press, London, p 213

    Google Scholar 

  8. Jianren Y, Yongquan Z, Shuzshen J, Zhengguo W, Fuzhen Z (1989) Gas chromatographic headspace determination of dithiocarbamate fungicide residues on vegetables. Sci Agric Sin 22:76–80

    Google Scholar 

  9. Kunkur VK, Hunje R, Biradar Patil NK, Vyakarnhal BS (2007) Effect of seed coating with polymer, fungicide and insecticide on seed quality in cotton during storage. Karnataka J Agric Sci 20(1):137–139

    Google Scholar 

  10. van Lishaut H, Schwack W (2000) Selective trace determination of dithiocarbamate fungicides in fruits and vegetables by reversed-phase ion-pair liquid chromatography with ultraviolet and electrochemical detection. J AOAC Int 83:720–727

    Google Scholar 

Download references


Authors are thankful to Indian Council of Agricultural Research (ICAR) for financial assistance under Emeritus Scientist Scheme, and to the Head, Division of Agricultural Chemicals, IARI, New Delhi for providing necessary facilities.

Author information



Corresponding author

Correspondence to Suresh Walia.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Walia, S., Sharma, R.K. & Parmar, B.S. Isolation and Simultaneous LC Analysis of Thiram and Its Less Toxic Transformation Product in DS Formulation. Bull Environ Contam Toxicol 83, 363–368 (2009).

Download citation


  • HPLC
  • Thiram
  • DS formulation
  • Transformation product