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Soil Pseudomonas Community Structure and Its Antagonism Towards Rhizoctonia solani Under the Stress of Acetochlor

Abstract

In a microcosm experiment, the amplified ribosomal DNA restriction analysis was adopted to investigate the Pseudomonas community structure in soils applied with different concentrations (0, 50, 150, and 250 mg/kg) of acetochlor, and an in vitro assay was made to examine the antagonistic activity of isolated Pseudomonas strains acting on soil-borne pathogen Rhizoctonia solani. The results showed that acetochlor application changed the community structure of Pseudomonas in aquic brown soil. The diversity of Pseudomonas and the amount of isolated Pseudomonas strains with antagonistic activity decreased with an increasing acetochlor concentration, and the toxic effect of acetochlor reached to a steady level at 150–250 mg/kg.

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Acknowledgments

This research was financially supported by the National Science Foundation for Yong Scientists of China (NO. 407010881). Pseudomonas fluorescens Q2-87 was kindly provided by Dr. Linda Thomashow, USDA, ARS Root Disease and Biological Control Research Unit.

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Correspondence to Qixing Zhou.

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Wu, M., Zhang, X., Zhang, H. et al. Soil Pseudomonas Community Structure and Its Antagonism Towards Rhizoctonia solani Under the Stress of Acetochlor. Bull Environ Contam Toxicol 83, 313–317 (2009). https://doi.org/10.1007/s00128-009-9731-7

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Keywords

  • Acetochlor
  • Pseudomonas
  • Soil microbial community
  • Antagonistic activity