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Environmental Hazard Assessment of Cheese Manufacturing Effluent Treated for Hydrogen Production


Toxicity of effluents after treatment in an anaerobic fermentation system for hydrogen production is evaluated with three biotests: The zebrafish Danio rerio embryo test, the Thamnotoxkit F and the Daphtoxkit FTM magna. Samples were classified from “very” to “extremely toxic”. Average toxicity values for zebrafish were 1.55% (24 h) and 0.75% (48 h), for Thamnocephalus 0.69% (24 h) and for Daphnia 2.51% (24 h) and 1.82% (48 h). Statistical analysis between physicochemical parameters and LC50 values revealed that PO −34 , SO −24 , NH3N and NO3 have the major contribution to toxicity. Based on results, this treatment is considered an environmentally ineffective way of managing the specific wastes.

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This paper is part of the 03ED375 research project, implemented within the framework of the “Reinforcement Programme of Human Research Manpower” (PENED) and co-financed by National and Community Funds (20% from the Greek Ministry of Development-General Secretariat of Research and Technology and 80% from E.U.-European Social Fund).

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Correspondence to Joan Iliopoulou-Georgudaki.

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Karadima, C., Theodoropoulos, C. & Iliopoulou-Georgudaki, J. Environmental Hazard Assessment of Cheese Manufacturing Effluent Treated for Hydrogen Production. Bull Environ Contam Toxicol 83, 428–434 (2009).

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  • Toxicity
  • Cheese whey
  • Thamnotoxkit F
  • Daphtoxkit FTM magna
  • Zebrafish
  • Anaerobic digestion