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Quantitative Study of Leydig Cell Populations in Mice Exposed to Low Doses of Cadmium


Leydig cell morphological changes were evaluated using morphometric and stereological methods in male mice exposed to low doses of cadmium. A possible reversibility of the changes after cadmium withdrawal was also considered. Nuclear morphological parameters and stereological densities of the Leydig cell population were lower in the cadmium-exposed groups than in the control. The withdrawal of cadmium did not lead to any significant recovery of the morphological parameters. Nevertheless, numerical density increased significantly in the withdrawn groups, suggesting that the hyperplasia of interstitial cells could try to relieve morphological damage after cadmium withdrawal.

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  1. Bench G, Corzett MH, Martinelli R, Balhorn R (1999) Cadmium concentrations in the testes, sperm, and spermatids of mice subjected to long-term cadmium chloride exposure. Cytometry 35:30–36. doi:10.1002/(SICI)1097-0320(19990101)35:1<30::AID-CYTO5>3.0.CO;2-M

    Article  CAS  Google Scholar 

  2. Benoff S, Jacob A, Hurley IR (2000) Male infertility and environmental exposure to lead and cadmium. Hum Reprod Update 6:107–121. doi:10.1093/humupd/6.2.107

    Article  CAS  Google Scholar 

  3. Biswas NM, Sen GR, Chattopadhyay A, Choudhury GR, Sarkar M (2001) Effect of atenolol on cadmium-induced testicular toxicity in male rats. Reprod Toxicol 15:699–704. doi:10.1016/S0890-6238(01)00184-8

    Article  CAS  Google Scholar 

  4. Blanco A, Moyano R, Vivo J, Flores-Acuña R, Molina A, Blanco C, Agüera E, Monterde JG (2007) Quantitative changes in the testicular structure in mice exposed to low doses of cadmium. Environ Toxicol Pharmacol 23:96–101. doi:10.1016/j.etap.2006.07.008

    Article  CAS  Google Scholar 

  5. Gouveia MA (1988) The testes in cadmium intoxication: morphological and vascular aspects. Andrologia 20:225–231

    CAS  Google Scholar 

  6. Gundersen HJ, Jensen EB (1985) Stereological estimation of the volume-weighted mean volume of arbitrary particles observed on random sections. J Microsc 138:127–142

    CAS  Google Scholar 

  7. Koizumi T, Li ZG, Tatsumoto H (1992) DNA damaging activity of cadmium in Leydig cells, a target cell population for cadmium carcinogenesis in the rat testis. Toxicol Lett 63:211–220. doi:10.1016/0378-4274(92)90013-A

    Article  CAS  Google Scholar 

  8. Selypes A, Serenyi P, Boldog I, Bokros F, Takacs S (1992) Acute and “long-term” genotoxic effects of CdCl2 on testes of mice. J Toxicol Environ Health 36:401–409

    CAS  Article  Google Scholar 

  9. Swiergosz-Kowalewska R (2001) Cadmium distribution and toxicity in tissues of small rodents. Microsc Res Tech 55:208–222. doi:10.1002/jemt.1171

    Article  CAS  Google Scholar 

  10. Teiichiro A, Hirmichi I, Keisuke M, Kunihiro H, Makoto H (2002) Cadmium-induced testicular damage in a rat model of subchronic intoxication. Reprod Med Biol 1:59–63. doi:10.1046/j.1445-5781.2002.00010.x

    Article  Google Scholar 

  11. Waalkes MP (2003) Cadmium carcinogenesis. Mutat Res 533:107–120. doi:10.1016/j.mrfmmm.2003.07.011

    CAS  Google Scholar 

  12. Yang JM, Arnush M, Chen QY, Wu XD, Pang B, Jiang XZ (2003) Cadmium-induced damage to primary cultures of rat Leydig cells. Reprod Toxicol 17:553–560. doi:10.1016/S0890-6238(03)00100-X

    Article  CAS  Google Scholar 

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Correspondence to J. G. Monterde.

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Blanco, A., Moyano, M.R., Molina, A.M. et al. Quantitative Study of Leydig Cell Populations in Mice Exposed to Low Doses of Cadmium. Bull Environ Contam Toxicol 82, 756–760 (2009).

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  • Cadmium
  • Testes
  • Stereology
  • Leydig