Skip to main content

Bioremediation of Reclaimed Wastewater Used as Landscape Water by Using the Denitrifying Bacterium Bacillus cereus


Organic matter and nitrogen removal from reclaimed wastewater used as landscape water was carried out by in situ bioremediation. A denitrifying bacterium Bacillus cereus DNF409 was introduced for this purpose, and the amount of B. cereus used was optimized. The total nitrogen (TN) content and chemical oxygen demand (COD) of the landscape water decreased from 9.86 to 3.1 mg/L (removal rate, 68.6%) and from 127 to 36 mg/L (removal rate, 71.7%). The transparency of water increased from 0.2 to 0.55 m.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3


  1. AWWA (1999) Standard methods for the examination of water and wastewater. AWWA, Washington

    Google Scholar 

  2. Bixio D, Thoeye C, De Koning J, Joksimovic D, Savic D, Wintgens T, Melin T (2006) Wastewater reuse in Europe. Desalination 187:89–101. doi:10.1016/j.desal.2005.04.070

    Article  CAS  Google Scholar 

  3. Burford MA, Thompson PJ, McIntosh RP, Bauman RH, Pearson DC (2003) Nutrient and microbial dynamics in high-intensity, zero-exchange shrimp ponds in Belize. Aquaculture 219:393–411. doi:10.1016/S0044-8486(02)00575-6

    Article  Google Scholar 

  4. Coveney MF, Stites DL, Lowe EF, Battoe LE, Conrow R (2002) Nutrient removal from eutrophic lake water by wetland filtration. Ecol Eng 19:141–159. doi:10.1016/S0925-8574(02)00037-X

    Article  Google Scholar 

  5. Devaraja TN, Yusoff FM, Shariff M (2002) Changes in bacterial population and shrimp production in ponds treated with commercial microbial products. Aquaculture 206:245–256. doi:10.1016/S0044-8486(01)00721-9

    Article  Google Scholar 

  6. Hallin S, Rothman M, Pell M (1996) Adaptation of denitrifying bacteria to acetate and methanol in activated sludge. Wat Res 30:1445–1450. doi:10.1016/0043-1354(96)00033-4

    Article  CAS  Google Scholar 

  7. Queiroz JF, Boyd CE (1998) Effects of a bacterial inoculum in channel catfish ponds. J World Aquac Soc 29:67–73. doi:10.1111/j.1749-7345.1998.tb00300.x

    Article  Google Scholar 

  8. Vezzulli L, Pruzzo C, Fabiano M (2004) Response of the bacterial community to in situ bioremediation of organic-rich sediments. Mar Pollut Bull 49:740–751. doi:10.1016/j.marpolbul.2004.05.010

    Article  CAS  Google Scholar 

  9. Wang L, Li J, Kang WL (2007) Bioremediation of eutrophicated water by Acinetobacter calcoaceticus. Bull Environ Contam Toxicol 78:527–530. doi:10.1007/s00128-007-9169-8

    Article  CAS  Google Scholar 

  10. Yang H, Abbaspour KC (2007) Analysis of wastewater reuse potential in Beijing. Desalination 212:238–250. doi:10.1016/j.desal.2006.10.012

    Article  CAS  Google Scholar 

  11. Zhao S, Hu N, Huang J, Liang Y, Zhao B (2008) High-yield spore production from Bacillus licheniformis by solid state fermentation. Biotechnol Lett 30:295–297. doi:10.1007/s10529-007-9540-1

    Article  Google Scholar 

Download references


This research was supported by the National Hi-Tech Development Program “863” project (No. 2006AA020201).

Author information



Corresponding author

Correspondence to Yunxiang Liang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zhao, S., Hu, N., Chen, Z. et al. Bioremediation of Reclaimed Wastewater Used as Landscape Water by Using the Denitrifying Bacterium Bacillus cereus . Bull Environ Contam Toxicol 83, 337–340 (2009).

Download citation


  • Bacillus cereus
  • Bioremediation
  • Landscape water
  • Reclaimed wastewater