This study evaluated the suitability of sealed containers for toxicity testing to prevent loss of volatile chlorinated hydrocarbons (VCHs) with a range of Australian marine organisms including: micro-algae; sea urchin and oyster larvae in 44 mL sealed vials and fish larvae; amphipods; and juvenile polychaetes in 1 L sealed jars. Vials prevented volatilisation of VCHs during testing. Jars were less effective, with average losses of 46%. Growth and development of algae, sea urchins and oysters in vials was acceptable, indicating suitability of the methodology. Jars were suitable for amphipods and polychaetes; however, further evaluation of the fish test is required.
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ANZECC and ARMCANZ (2000) Australian and New Zealand Guidelines for Fresh and Marine Water Quality. Australian and New Zealand Environment and Conservation Council and the Agriculture and Resource Management Council of Australia and New Zealand, National Water Quality Management Strategy, Canberra
Brack W, Rottler H, Frank H (1998) Volatile fractions of landfill leachates and their effect on Chlamydomonas reinhardtii: In vivo chlorophyll a fluorescence. Environ Toxicol Chem 17(10):1982–1991
Cohen A, Nugegoda D (2000) Toxicity of three oil spill remediation techniques to the Australian Bass Macquaria novemaculeata. Ecotoxicol Environ Saf 47:178–185
Galassi S, Vighi M (1981) Testing toxicity of volatile substances with algae. Chemosphere 10:1123–1126
Herman DC, Inniss WE, Mayfield CI (1990) Impact of volatile aromatic hydrocarbons, alone and in combination, on growth of freshwater algae Selenastrum capricornutum. Aquat Toxicol 18:87–100
Hogan AC, Stauber JL, Pablo F, Adams MS, Lim RP (2005) The development of marine toxicity identification evaluation (TIE) procedures using the unicellular alga Nitzschia closterium. Arch Environ Contam Toxicol 48:433–443
Hunt JE, Birch G, Warne MSJ (2007) Deriving trigger values for, and assessing hazard posed by, volatile chlorinated hydrocarbons in a Sydney estuary. Aust J Ecotoxicol 13:33–42
Lin J-H, Kao W-C, Tsai K-P, Chen C-Y (2005) A novel algal toxicity testing technique for assessing the toxicity of both metallic and organic toxicants. Water Res 39:1869–1877
Mayer P, Nyholm N, Verbruggen EMJ, Hermens JLM, Tolls J (2000) Algal growth inhibition test in filled, closed bottles for volatile and sorptive materials. Environ Toxicol Chem 19(10):2551–2556
Rose RM, Warne MSJ, Lim RP (1997) Inter-species conversion equations for predicting toxicity of non-polar narcotic chemicals to Ceriodaphnia dubia. Aust J Ecotoxicol 3:75–83
Tsai K-P, Chen C-Y (2007) An algal toxicity database of organic toxicants derived by a closed system technique. Environ Toxicol Chem 26(9):1931–1939
USEPA (1996) Test methods for evaluating solid waste; Revision 2 Vol B. US Environmental Protection Agency, National Technical Information Service, Washington, DC
USEPA (2002) Short term methods for estimating the chronic Toxicity of effluents and receiving waters to marine and estuarine organisms. US Environmental Protection Agency. Report nr EPA-821-R-02-014
Widdows J (1993) Marine and estuarine invertebrate toxicity tests. In: Calow P (ed) Handbook of Ecotoxicology, vol 1. Blackwell Scientific Publications, Oxford, pp 145–166, chapter 9
Financial support for this study was provided by Orica Australia Pty Ltd and analytical services were provided by ALS Environmental Pty Ltd.
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Hunt, J., Birch, G., Warne, M.S.J. et al. Evaluation of a Methodology for Toxicity Testing of Volatile Chlorinated Hydrocarbons on Marine Organisms. Bull Environ Contam Toxicol 82, 743–748 (2009). https://doi.org/10.1007/s00128-009-9678-8
- Volatile chlorinated hydrocarbons
- Toxicity testing
- Sealed containers