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Evaluation of a Methodology for Toxicity Testing of Volatile Chlorinated Hydrocarbons on Marine Organisms


This study evaluated the suitability of sealed containers for toxicity testing to prevent loss of volatile chlorinated hydrocarbons (VCHs) with a range of Australian marine organisms including: micro-algae; sea urchin and oyster larvae in 44 mL sealed vials and fish larvae; amphipods; and juvenile polychaetes in 1 L sealed jars. Vials prevented volatilisation of VCHs during testing. Jars were less effective, with average losses of 46%. Growth and development of algae, sea urchins and oysters in vials was acceptable, indicating suitability of the methodology. Jars were suitable for amphipods and polychaetes; however, further evaluation of the fish test is required.

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Financial support for this study was provided by Orica Australia Pty Ltd and analytical services were provided by ALS Environmental Pty Ltd.

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Correspondence to James Hunt.

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Hunt, J., Birch, G., Warne, M.S.J. et al. Evaluation of a Methodology for Toxicity Testing of Volatile Chlorinated Hydrocarbons on Marine Organisms. Bull Environ Contam Toxicol 82, 743–748 (2009).

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  • Volatile chlorinated hydrocarbons
  • Toxicity testing
  • Sealed containers