A simple method using high-performance liquid chromatography and fluorescence detection is presented for the simultaneous determination of the antiparasitic agents avermectins (abamectin, ivermectin and doramectin) in bovine liver. Samples were extracted using acetonitrile and cleaned up on an alumina SPE column and derivatized with trifluoroacetic anhydride. The derivatives were detected using fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. As the results, the limit of quantification for three avermectins was about 1 μg/kg. Inter-assay showed the mean recoveries of avermectins in bovine liver ranged from 72% to 81%, with RSD less than 9.9%.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Regulation No. 235 (2002) Ministry of Agriculture, People’s Republic of China, 24 Dec, Maximum residue limit in food of animal origin
Council Regulation (EEC) No. 2377/90 of 26 June 1990 (1990) Maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off J Eur Commun L224:1
David AD (2007) Positive and negative electrospray LC-MS/MS methods for quantitation of the antiparasitic endectocide drugs, abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin and selamectin in milk. J Chromatogr B 850:134–146. doi:10.1016/j.jchromb.2006.11.014
Pierre M, Shim Jung-Sook K, Pivnichny James V (1990) Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and N-methylimidazole as the derivatization reagent. J Pharm Biomed Anal 8:507–511. doi:10.1016/0731-7085(90)80060-3
Prieto JG, Merino G, Pulido MM, Estevez E, Molina AJ, Vila L, Alvarez AI (2003) Improved LC method to determine ivermectin in plasma. J Pharm Biomed Anal 31:639–645. doi:10.1016/S0731-7085(02)00720-3
Funding supports from Natural Science Foundation of China (20621703) and the Foundation of Ministry of Science & Technology of China (2002BA906A75) are gratefully acknowledged.
About this article
Cite this article
Wang, H., Wang, Z., Liu, S. et al. Rapid Method for Multi-residue Determination of Avermectins in Bovine Liver Using High-performance Liquid Chromatography with Fluorescence Detection. Bull Environ Contam Toxicol 82, 395–398 (2009). https://doi.org/10.1007/s00128-009-9638-3