An analytical method was developed to determine the phenoxyacid herbicides 2,4-D, MCPA and mecoprop in kidney tissue from animals where poisoning is suspected. Samples were Soxhlet extracted using diethyl ether and the extracts cleaned-up using anion exchange solid phase extraction cartridges. Analysis was performed using liquid chromatography with negative-ion electrospray tandem mass spectrometry (LC-MS/MS). The method was evaluated by analysing control kidney samples fortified at 1 and 5 mg/kg. Mean recoveries ranged from 82 to 93% with relative standard deviations from 3.2 to 19%. The limit of detection was estimated to be 0.02 mg/kg.
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Funding for this work was provided by the UK Department for Environment Food and Rural Affairs (Defra).
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Charlton, A.J.A., Stuckey, V. & Sykes, M.D. Determination of the Phenoxyacid Herbicides MCPA, Mecoprop and 2,4-D in Kidney Tissue Using Liquid Chromatography with Electrospray Tandem Mass Spectrometry. Bull Environ Contam Toxicol 82, 711–715 (2009). https://doi.org/10.1007/s00128-009-9636-5
- Acid herbicides
- Pesticide poisoning
- Liquid chromatography
- Mass spectrometry