Microcystin-LR (MC-LR) is a heptapeptide hepatotoxin produced by cyanobacteria. Immunoaffinity chromatography (IAC) column was prepared with CNBr-activated Sepharose 4B and monoclonal antibody of MC-LR. Water sample was cleaned up by IAC column and the content of MC-LR in water was determined by liquid chromatography-mass spectrometry (LC-MS). The results suggested that the IAC column exhibited highly selective specificity for MC-LR and selective removed interference from complex water sample. Water sample was concentrated for 2,000-fold through once purification. Cyanobacterial blooms had broken out in 2007 in Lake Tai, the third largest freshwater lake in China. Water samples from two parts of Lake Tai had been analyzed. The highest concentration of MC-LR in water from Lake Tai was 0.522 μg/L.
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We would like to express our gratitude to Tao GuanJun and Qin Fang (Jiangnan University) for their help in LC-MS, and thank Dr. F.S., Chu and Dr. Richard Hirsh for their help in preparation for manuscript. This study is supported by the Ministry of Health, China (No. WKJ2006-2-10).
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Xiao, FG., Zhao, XL., Tang, J. et al. Determination of Microcystin-LR in Water from Lake Tai, China. Bull Environ Contam Toxicol 82, 230–233 (2009). https://doi.org/10.1007/s00128-008-9623-2
- Cyanobacterial blooms
- Immunoaffinity chromatography
- Lake Tai
- Liquid chromatography-mass spectrometry