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Effect of Cypermethrin on Some Developmental Stages of Drosophila melanogaster

Abstract

This study investigated the effects of cypermethrin, a synthetic pyrethroid, on some developmental features of Drosophila melanogaster. Before the experiment the lethal concentration of this insecticide was determined. Cypermethrin solution was applied to Drosophila melanogaster by means of nutrition, by adding it to the culture medium. In the control group no such substance was applied. Our aim was to find out whether any developmental toxic effects occur, and, if they do, during which stage, by observing the rates of egg-laying of adult females, the development of eggs, and the development of larvae. The results showed that there was no significant difference in the rates of egg-laying. However, the decrease in the rate of egg development revealed that eggs and early embryonic stages were sensitive to toxic effects. The same toxic effect was not observed in third instar larvae. The toxic effect was observed to be strongest in the early stages of development.

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References

  1. Batiste-Alentorn M, Xamena N, Vélazquez A, Creus A, Marcos R (1987) Studies on the toxicity of cypermethrin and fenvalerate in different strains of Drosophila melanogaster Meig. (Insecta, Diptera). Environ Res 43:117–125. doi:10.1016/S0013-9351(87)80063-4

    Article  CAS  Google Scholar 

  2. El-Toukhy MA, Girgis RS (1993) In vivo and in vitro studies on the effect of larvin and cypermethrin on adenosine triphosphatase activity of male rats. J Environ Sci Health B 28(5):599–619

    Article  CAS  Google Scholar 

  3. Greulich K, Pflugmacher S (2003) Differences in susceptibility of various life stages of amphibian to pesticide exposure. Aquat Toxicol 65:329–336. doi:10.1016/S0166-445X(03)00153-X

    Article  CAS  Google Scholar 

  4. Gelegen L, Yeşilada E (2000) Drosophila melanogaster’ in bazı gelişimsel özellikleri üzerine kadmiyum nitratın etkisi. Turk J of Biol 24:585–591

    CAS  Google Scholar 

  5. Institóris L, Siroki O, Undeger U, Dési I, Nagymajtenyi L (1999) Immunotoxicological effects of repeated combined exposure by cypermethrin and the heavy metals lead and cadmium in rats. Int J Immunopharmacol 21:735–743. doi:10.1016/S0192-0561(99)00049-1

    Article  Google Scholar 

  6. Kaya B, Creus A, Yanikoğlu A, Cabré O, Marcos R (2000) Use of the Drosophila wing spot test in the genotoxicity testing of different herbicides. Environ Mol Mutagen 36:40–46. doi:10.1002/1098-2280(2000)36:1<40::AID-EM6>3.0.CO;2-K

    Article  CAS  Google Scholar 

  7. Kryger U, Deschodt C, Davis AL, Scholtz CH (2006) Effects of cattle treatment with a cypermethrin/cymiazol spray on survival and reproduction of the dung beetle species Euoniticellus intermedius (Coleoptera: Scarabaeidae). Bull Entomol Res 96(6):597–603. doi:10.1017/BER2006463

    Article  CAS  Google Scholar 

  8. Marchal-Segault D (1993) Effects de preparations commerciales de benomyl, mancozebe, sulfate de cuivre et diclofluanide sur la fecondite de Drosophila melanogaster Meigen. J Environ Sci Health B 28(4):397–411

    Article  CAS  Google Scholar 

  9. Miadoková E, Vlčková V, Duhová V, Trebatická M, Garajová L, Grolmus J, Podstavková S, Vlečk D (1992) Effects of supercypermethrin, a synthetic developmental pyrethroid, on four biological test systems. Mutat Res 280:161–168. doi:10.1016/0165-1218(92)90044-Z

    Article  Google Scholar 

  10. Mukhopadhyay I, Nazir A, Saxena DK, Chowdhuri DK (2002) Toxicity of cypermethrin: hsp70 as a biomarker of response in transgenic Drosophila. Biomarkers 7(6):501–510. doi:10.1080/1354750021000034852

    Article  CAS  Google Scholar 

  11. Mukhopadhyay I, Chowdhuri DK, Bajpayee M, Dhawan A (2004) Evaluation of in vivo genotoxicity of cypermethrin in Drosophila melanogaster using the alkaline Comet assay. Mutagenesis 19(2):85–90. doi:10.1093/mutage/geh007

    Article  CAS  Google Scholar 

  12. Mukhopadhyay I, Siddique HR, Bajpai VK, Saxena DK, Chowdhuri DK (2006) Synthetic pyrethroid cypermethrin induced cellular damage in reproductive tissues of Drosophila melanogaster: hsp70 as a marker of cellular damage. Arch Environ Contam Toxicol 51:673–680. doi:10.1007/s00244-005-0169-6

    Article  CAS  Google Scholar 

  13. Nadda G, Saxena PN, Srivastava G (2005) Effects of sublethal doses of beta-cyfluthrin on mutant Drosophila melanogaster (Diptera: Drosophilidae). Appl Entomol Zool 40(2):265–271. doi:10.1303/aez.2005.265

    Article  CAS  Google Scholar 

  14. Nazir A, Mukhopadhyay I, Saxena DK, Chowdhuri DK (2001) Chlorpyrifos-induced hsp70 expression and effect on reproductive performance in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Arch Environ Contam Toxicol 41:443–449. doi:10.1007/s002440010270

    Article  CAS  Google Scholar 

  15. Özkan F (1995) Oral yolla alınan organofosfatlı insektisit Malathion’un Pimpla turionellae L. dişi bireylerinin yaşam süresi, yumurta verimi ve açılımına etkisi. Msc. Thesis, Cukurova University Institute of Basic and Applied Sciences 62–78 Adana

  16. Partridge L, Green A, Fowler K (1987) Effects of egg-production and exposure to males on female survival in Drosophila melanogaster. J Insect Physiol 33(10):745–749. doi:10.1016/0022-1910(87)90060-6

    Article  Google Scholar 

  17. Rani S, Tabassum R, Naqvi SNH, Ahmad I, Sultan B, Khan MF (1997) Comparative toxicity of different formulations of cypermethrin, deltamethrin and permethrin against Drosophila melanogaster (KU strain). J Environ Biol 18(4):343–349

    CAS  Google Scholar 

  18. Uysal H, Kaya Y (2004) Toxicity of Eupharbia canariensis latex to some developmental stages of Drosophila melanogaster (Diptera:Drosophilidae). Bull Environ Contam Toxicol 72(1):45–53. doi:10.1007/s00128-003-0239-2

    Article  CAS  Google Scholar 

  19. Vélazquez A, Xamena N, Creus A, Marcos R (1990) Mutagenic evaluation of the organophosphorus insecticides methyl parathion and triazophos in Drosophila melanogaster. J Toxicol Environ Health 31:313–325

    Article  Google Scholar 

  20. Ware GW (1983) Pesticides theory and application. Freeman, San Francisco

    Google Scholar 

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Correspondence to Ayla Karataş.

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Karataş, A., Bahçeci, Z. Effect of Cypermethrin on Some Developmental Stages of Drosophila melanogaster . Bull Environ Contam Toxicol 82, 738–742 (2009). https://doi.org/10.1007/s00128-008-9604-5

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Keywords

  • Drosophila
  • Cypermethrin
  • Developmental features