Skip to main content

Quantification of Nitroimidazoles Residues in Swine Liver by Liquid Chromatography–Mass Spectrometry with Atmospheric Pressure Chemical Ionization

Abstract

A liquid chromatography–mass spectrometry method was developed for the simultaneous determination of ronidazole (RNZ), metronidazole (MNZ) and dimetridazole (DMZ) residues in swine liver. Following liquid–liquid extraction, the HLB solid-phase extraction was used for further purification. The targets were detected by atmospheric pressure chemical ionization (APCI) following the reverse phase liquid chromatography separation. Consequently, the detection limits for the method were 0.5 μg/kg for MNZ, 1.0 μg/kg for RNZ and 0.5 μg/kg for DMZ, respectively. The accuracies were determined using swine liver samples fortified at levels of 0.5, 1, 2, and 4 μg/kg and the mean recoveries of the analytes were between 66% and 81%.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Animal Medicinal Drug Use Clarification Act (1994) U.S.

  2. Anne-Catherine H, Leen M, Els D, Terrence F, Christopher E, Philippe D (2005) Development of an ELISA screening test for nitroimidazoles in egg and chicken muscle. Anal Chim Acta 534:157–162. doi:10.1016/j.aca.2004.06.037

    Article  Google Scholar 

  3. Commission Decision (2002) 2002/657/EC, EC

  4. Council Regulation no (1990) 2377/90, EC

  5. Dominique H, Bernard D, Michel L (2000) Determination of four nitroimidazole residues in poultry meat by liquid chromatography–mass spectrometry. J Chromatogr A 882:89–98. doi:10.1016/S0021-9673(00)00336-8

    Article  Google Scholar 

  6. IARC (1987) Monographs on the evaluation of carcinogenic risks to humans. Overall evaluations of carcinogenicity. Supplement 7

  7. Regulation No 193 (2002) Ministry of agriculture, P.R. China

  8. Polzer J, Gowik P (2001) Validation of a method for the detection and confirmation of nitroimidazoles and corresponding hydroxy metabolites in turkey and swine muscle by means of gas chromatography–negative ion chemical ionization mass spectrometry. J Chromatogr B Biomed Sci Appl 761:47–60. doi:10.1016/S0378-4347(01)00299-7

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Funding supports from Natural Science Foundation of China (20621703) and the Foundation of Ministry of Science & Technology of China (2002BA906A75) are gratefully acknowledged.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Hai Wang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wang, H., Wang, Z., Liu, S. et al. Quantification of Nitroimidazoles Residues in Swine Liver by Liquid Chromatography–Mass Spectrometry with Atmospheric Pressure Chemical Ionization. Bull Environ Contam Toxicol 82, 411–414 (2009). https://doi.org/10.1007/s00128-008-9591-6

Download citation

Keywords

  • Nitroimidazoles
  • LC–APCI–MS
  • Swine liver