Skip to main content
SpringerLink
Log in

Heavy Metal Resistant of E. coli Isolated from Wastewater Sites in Assiut City, Egypt

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Twelve isolates of E. coli were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weights 27 and 65 kb for hexa- and trivalent chromium. It was identified as E. coli ASU 7. Its minimal inhibitory concentration (MIC) for Cu2+, Co2+, Ni2+, Zn2+, Cr6+, Cr3+, Cd2+ and Pb2+ were 1.57, 2.55, 1.7, 9.17, 0.48, 7.69, 4.4 and 3.1 mM, respectively. Growth kinetics was determined under Cr6+ and Cr3+ stress. SDS-PAGE of protein profile shows that 10 ppm (0.19 mM) of Cr6+ induces new protein with molecular weight 23 kDa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Babich H, Stotzky G (1977) Sensitivity of various bacteria, including actinomycetes, and fungi to cadmium and the influence of pH on sensitivity. Appl Environ Microbiol 33:681–695

    CAS  Google Scholar 

  • Baur AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disc diffusion method. Am J Clin Pathol 45:493–496

    Google Scholar 

  • Bergey’s Manual of Systematic Bacteriology (2005) Volume two: the proteobacteria part C. Springer Verlag, New York

    Google Scholar 

  • Bezverbnaya IP, Buzoleva LS, Khristoforova NS (2005) Metal-resistant heterotrophic bacteria in coastal waters of primorye. Russ J Mar Biol 31:73–77. doi:10.1007/s11179-005-0047-0

    Article  Google Scholar 

  • Bhinde JV, Dhakephalkar PK, Paknikar KM (1996) Microbiological process for the removal of Cr(VI) from chromate bearing cooling tower effluent. Biotechnol Lett 18:667–672. doi:10.1007/BF00130763

    Article  Google Scholar 

  • Birnboimm HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523. doi:10.1093/nar/7.6.1513

    Article  Google Scholar 

  • Bishop PL (2002) Pollution prevention: fundamentals and practice. Beijing Tsinghua University Press

  • Bruins MR, Kapil S, Oehme FW (2000) Microbial resistance to metals in the environment. Ecotoxicol Environ Saf 45:198–207. doi:10.1006/eesa.1999.1860

    Article  CAS  Google Scholar 

  • Cervantes C, Campos-Garcia J, Devars S, Gutierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R (2001) Interactions of chromium with microorganisms and plants. FEMS Microbiol Rev 25:335–47. doi:10.1111/j.1574-6976.2001.tb00581.x

    Article  CAS  Google Scholar 

  • Dhakephalker PK, Chopade BA (1994) High levels of multiple metal resistances and its correlation to antibiotics resistance in environmental isolates of Acinetobacter. Biometals 7:67–74

    Google Scholar 

  • Ehrlich HL (1997) Microbes and metals. Appl Microbiol Biotechnol 48:687–692. doi:10.1007/s002530051116

    Article  CAS  Google Scholar 

  • Elith M, Garwood S (2001) Investigation into the levels of heavy metals within the manly dam catchment. In: Freshwater ecology report. Department of Environmental Sciences University of Technology, Sydney

  • Filali BK, Taoufik J, Zeroual Y, Dzairi FZ, Talbi M, Blaghen M (2000) Wastewater bacterial isolates resistant to heavy metals and antibiotics. Curr Microbiol 41:151–156

    Article  CAS  Google Scholar 

  • Ganguli A, Tripathi AK (2002) Bioremediation of toxic chromium from electroplating effluent by chromate reducing Pseudomonas aeruginosa A2Chr in two bioreactors. Appl Microbiol Biotechnol 58:416–420. doi:10.1007/s002530100822

    Article  CAS  Google Scholar 

  • Ghosh S, Mahapatra NR, Banerjee PC (1997) Metal resistance in Acidocella strains and plasmid-mediated transfer of this characteristic to Acidiphilium multivorum and Escherichia coli. Appl Environ Microbiol 11:4523–4527

    Google Scholar 

  • Hassen A, Saidi N, Cherif M, Boudabous A (1998) Resistance of environmental bacteria to heavy metals. Bioresour Technol 64:7–15. doi:10.1016/S0960-8524(97)00161-2

    Article  CAS  Google Scholar 

  • Hughes MN, Poole RK (1989) Metals and micro-organism. Chapman and Hall, London

    Google Scholar 

  • Hussein H, Farag S, Kandil K, Moawad H (2005) Resistance and uptake of heavy metals by Pseudomonads. Process Biochem 40:955–961. doi:10.1016/j.procbio.2004.04.001

    Article  CAS  Google Scholar 

  • Laemmli VK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 277:680–685. doi:10.1038/227680a0

    Article  Google Scholar 

  • Ledrich ML, Stemmler S, Gilly PL, Foucaud L, Falla J (2005) Precipitation of silver-thiosulfate complex and immobilization of silver by Cupriavidus metallidurans CH34. BioMetals 18:643–650. doi:10.1007/s10534-005-3858-8

    Article  CAS  Google Scholar 

  • Liu GY, Xu WH, Zeng MG, Li X, Gao H (2006) Cr(VI) reduction by Bacillus sp. isolated from chromium landfill. Process Biochem 41:1981–1986. doi:10.1016/j.procbio.2006.04.020

    Article  CAS  Google Scholar 

  • MacFaddin J (1985) Media for isolation-cultivation-identification-maintenance of medical bacteria, vol 1. Williams and Wilkins, Baltimore, Maryland

    Google Scholar 

  • Mergeay M (1995) Heavy metal resistance in microbial ecosystems. Mol Microb Ecol Man 6:7–17

    Google Scholar 

  • Nies DH (1999) Microbial heavy-metal resistance. Appl Microbiol Biotechnol 51:730–750. doi:10.1007/s002530051457

    Article  CAS  Google Scholar 

  • Nies DH (2003) Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev 27:313–339. doi:10.1016/S0168-6445(03)00048-2

    Article  CAS  Google Scholar 

  • Pal A, Choudhuri P, Dutta S, Mukherjee PK, Paul AK (2004) Isolation and characterization of nickel-resistant microflora from serpentine soils of Andaman. World J Microbiol Biotechnol 20:881–886. doi:10.1007/s11274-004-2776-1

    Article  CAS  Google Scholar 

  • Patel JS, Patel PC Kalia K (2006) Isolation and characterization of nickel uptake by nickel resistant bacterial isolate (NiRBI). Biomed Environ Sci 19:297–301

    CAS  Google Scholar 

  • Piotrowska-Seget Z, Cycon M, Kozdroj J (2005) Metal-tolerant bacteria occurring in heavily polluted soil and mine spoil. Appl Soil Ecol 28:237–246. doi:10.1016/j.apsoil.2004.08.001

    Article  Google Scholar 

  • Poole RK, Gadd GM (1989) Metals: microbe interactions. Programme on chemical safety (concise international chemical assessment document 12). IRL Press, Oxford, p 137

  • Ramteke PW (1997) Plasmid mediated co-transfer of antibiotic resistance and heavy metal resistance in coliforms, Indian J Med Microbiol 37:177–181

    Google Scholar 

  • Rossbach S, Kukuk ML, Wilson TL, Feng SF, Pearson MM, Fisher MA (2000) Cadmium-regulated gene fusions in Pseudomonas fluorescens. Environ Microbiol 2:373–382. doi:10.1046/j.1462-2920.2000.00117.x

    Article  CAS  Google Scholar 

  • Sabry SA, Ghozlan HA, Abou-zeid DM (1997) Metal resistance and antibiotic resistance patterns of bacterial population isolated from sea water. J Appl Microbiol 82:245–252

    CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Sharma R, Rensing C, Rosen BP, Mitra B (2000) The ATP hydrolytic activity of purified ZntA, a Pb(II),Cd(II), Zn(II)-translocating ATPase from Escherichia coli. J Biol Chem 275:3873–3878. doi:10.1074/jbc.275.6.3873

    Article  CAS  Google Scholar 

  • Soltan EM (2001) Isolation and characterization of antibiotic and heavy metal resistant Pseudomonas aeruginosa from different polluted waters in Sohag district, Egypt. J Microbiol Biotechnol 11:50–553

    CAS  Google Scholar 

  • Spain A, (2003) Implications of microbial heavy metal resistance in the environment. Review of Undergraduate Research 2:1–6

    Google Scholar 

  • Thacker U, Parikh R, Shouche Y, Madamwar D (2007) Reduction of chromate by cell-free extract of Brucella sp. Isolated from Cr(VI) contaminated sites. Bioresour Technolol 98:1541–1547. doi:10.1016/j.biortech.2006.06.011

    Article  CAS  Google Scholar 

  • Ünaldi MN, Korkmaz H, Arikan B, Coral G (2003) Plasmid-encoded heavy metal resistance in Pseudomonas sp. Bull Environ Contam Toxicol 71:1145–1150. doi:10.1007/s00128-003-8575-9

    Article  CAS  Google Scholar 

  • Volesky B (1990) Biosorption and biosorbents. In: Volesky B (ed) Biosorption of heavy metals. CRC press, Florida, pp 3–5

    Google Scholar 

  • Washington JA, Sutter VL (1981) Dilution test procedures. In: Lennette EH, Balows A, Hausler WJ, Truant JP (eds) Manual of clinical microbiology. American society for microbiology, Washington, District of Columbia, pp 549–555

    Google Scholar 

  • WHO (World Health Organization) (2005) Guidelines for drinking-water quality, vol 1. WHO, Geneva

    Google Scholar 

  • Wuertz S, Mergeay M (1997) The impact of heavy metals on soil microbial communities and their activities. In: van Elsas JD, Trevors JT, Wellington EMH (eds) Modern soil microbiology. Marcel Dekker, New York

    Google Scholar 

  • Yilmaz EI (2003) Metal resistance and biosorption capacity of Bacillus circulans strain EB1. Res Microbiol 154:409–415. doi:10.1016/S0923-2508(03)00116-5

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Science, Botany Department, University of Assiut, 71516, Assiut, Egypt

    R. N. N. Abskharon, S. H. A. Hassan, S. M. F. Gad El-Rab & A. A. M. Shoreit

Authors
  1. R. N. N. Abskharon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. H. A. Hassan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. M. F. Gad El-Rab
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. M. Shoreit
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. A. M. Shoreit.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abskharon, R.N.N., Hassan, S.H.A., Gad El-Rab, S.M.F. et al. Heavy Metal Resistant of E. coli Isolated from Wastewater Sites in Assiut City, Egypt. Bull Environ Contam Toxicol 81, 309–315 (2008). https://doi.org/10.1007/s00128-008-9494-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-008-9494-6

Keywords

Search

Navigation