A simple, rapid and sensitive spectrophotometric method is described for the determination of hydrogen peroxide using toluidine blue as a reagent. The proposed method is based on the liberation of iodine in acidic medium equivalent to the amount of hydrogen peroxide present. The liberated iodine bleaches the blue color of toluidine blue and is measured at 628 nm. The decrease in absorbance is directly proportional to hydrogen peroxide concentration and obeys Beer’s law in the range 0.2 × 10–6 –14 × 10−6 mol L−1. The molar absorptivity, sandel’s sensitivity, detection limit and quantitation limit of the method were found to be 1.82 × 104 L mol−1 cm−1, 2.01 × 10−9 mol L−1, 1.41 × 10−6 mol L−1 and 4.28 × 10−6 mol L−1 respectively. The results of analysis of the proposed method are compared favorably with those from a reference method.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Almuaibed AM, Townshend A (1994) Flow spectrophotometric method for determination of hydrogen peroxide using a cation exchanger for preconcentration. Anal Chim Acta 295:159–163. doi:10.1016/0003-2670(94)80346-3
Baga AN, Ayad N, Johnson GR, Alastair NNB Rajiha AS (1988) A simple spectrophotometric determination of hydrogen peroxide at low concentrations in aqueous solution. Anal Chim Acta 204:349–353. doi:10.1016/S0003-2670(00)86374-6
Bethesda MD (1995) Hazardous substances data bank: hydrogen peroxide. National Library of Medicine, NLM
Bowers LD (1986) Application of immobilized biocataysts in chemical analysis. Anal Chem 58:513–530. doi:10.1021/ac00295a001
Clapp PA, Evans DF (1991) Spectrophotometric determination of hydrogen peroxide with leuco patient blue violet. Anal Chim Acta 243:217–220. doi:10.1016/S0003-2670(00)82563-5
Cosogrove M, Moody GJ, Thomas JDR (1988) Chemicaly immobilized enzyme electrodes for hydrogen peroxide determination. Analyst 113:1811– 1815. doi:10.1039/an9881301811
Ibusuki T (1983) Influence of trace metal ions on the determination of hydrogen peroxide in rain water by using a chemiluminescent technique. Atoms Environ 17:393–396. doi:10.1016/0004-6981(83)90056-2
Jeffery GH, Bassett J, Mendham J, Denney RC (1995) Vogel’s textbook of quantitative chemical analysis, 5th edn. ELBS, Longman, London
Jie N, Yang J, Huang X, Zhang R, Song Z (1995) Fluorimetric determination of hydrogen peroxide in water using acetaminophen. Talanta 42:1575–1579. doi:10.1016/0039-9140(95)01603-1
Li Y (1996) Biological properties of peroxide-containing tooth whiteners. Food Chem Toxicol 34:887–904. doi:10.1016/S0278-6915(96)00044-0
Meyer J, Karst U (1999) Workplace monitoring of gas phase hydrogen peroxide by means of fluorescence spectroscopy. Anal Chim Acta 401:191–196. doi:10.1016/S0003-2670(99)00488-2
Newman (ed) (1993) Measurement challenges in atmospheric chemistry. American Chemical Society, Washington, DC
Nogueira RFP, Oliveira MC, Paterlini WC (2005) Simple and fast spectrophotometric determination of H2O2 in photo-fenton reactions using metavanadate. Talanta 66:86–91. doi:10.1016/j.talanta.2004.10.001
Pappas ACh, Stalikas CD, Fiamegos YCh, Karayannis MI (2002) Determination of hydrogen peroxide by using a flow injection system with immobilized peroxidase and long pathlength capillary spectrophotometry. Anal Chim Acta 455:305–313. doi:10.1016/S0003-2670(01)01600-2
Tanner PA, Wong AYS (1998) Spectrophotometric determination of hydrogen peroxide in rainwater. Anal Chim Acta 370:279–287. doi:10.1016/S0003-2670(98)00273-6
Willey JD, Kieber RJ, Lancaster RD (1996) Coastal rainwater hydrogen peroxide: concentration and deposition. J Atmos Chem 25:149–165. doi:10.1007/BF00053789
Wolfe WC (1962) Spectrophotometric determination of hydroperoxide in diethyl ether. Anal Chem 34:1328–1330
Yuan H, Cai R, Pan Z (2003) Catalytic kinetic determination of hydrogen peroxide using Iron tetrasulfonatophthalocyanine (with g-CD) as a mimetic enzyme of peroxidase. Anal Lett 36:277–286. doi:10.1081/AL-120017686
Zhang K, Mao L, Cai R (2000) Stopped-flow spectrophotometric determination of hydrogen peroxide with hemoglobin as catalyst. Talanta 51:179–186. doi:10.1016/S0039-9140(99)00277-5
Authors thank the Microtron Centre of Mangalore University, Mangalore, for the technical help.
About this article
Cite this article
Sunil, K., Narayana, B. Spectrophotometric Determination of Hydrogen Peroxide in Water and Cream Samples. Bull Environ Contam Toxicol 81, 422–426 (2008). https://doi.org/10.1007/s00128-008-9477-7
- Hydrogen peroxide
- Toluidine blue