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Effects of EDTA on Lead Uptake by Typha orientalis Presl: A New Lead-Accumulating Species in Southern China


A series of field investigations have been conducted at Yongzhou Pb/Zn/Cu mine tailings, Hunan Province, southern China. The specific aim was to search for new lead accumulators with fast growth rate and large biomass. The results of tissue analyses identified Typha orientalis Presl has a strong accumulation of lead. The average lead concentrations in the leaves and roots are 619 and 1,233 mg/kg, respectively. The growth and Pb content of the plant were also studied by hydroponic culture with different concentrations of Pb(NO3)2. Growth of the plant was not affected by Pb up to 300 mg/L. The Pb concentrations in the leaves and roots increased with increasing of Pb level in the modified Hoagland’s nutrient solution. The maximum concentrations of Pb in the leaves and roots were 16,190 and 64,405 mg/kg, respectively. The study also demonstrated that EDTA not only increased the amount of Pb taken up by plants but also speeded up the metal translocation from roots to leaves.

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  1. Aneta P, Barbara T, Danuta B (2003) Enhancing phytoremediative ability of Pisum sativum by EDTA application. Phytochemistry 64:1239–1251

    Article  CAS  Google Scholar 

  2. Begonia GB, Davis MFT, Gray BCN (1998) Growth responses of Indian mustard and its phytoextraction of lead from a contaminated soil. Bull Environ Contam Toxicol 61:38–43

    Article  CAS  Google Scholar 

  3. Cunningham SD, Ow DW (1996) Promises and prospects of phytoermediation. Plant Physiol 110:715–719

    CAS  Google Scholar 

  4. Gabrielle T, Patrick JT (1996) Uptake and localization of lead in corn (Zea mays L.) seedlings, a study by histochemical and electron microscopy. Sci Total Environ 188:71–85

    Article  Google Scholar 

  5. He B, Yang XE, Ni WZ, Wei YZ (2002) Sedum alfredii: a new lead-accumulating ecotype. J Integr Plant Biol 44(11):1365–1370

    CAS  Google Scholar 

  6. Huang JW, Chen JJ (1997) Phytoremediation of lead-contaminated soils: role of synthetic chelates on lead phytoextraction. Environ Sci Technol 31:800–805

    Article  CAS  Google Scholar 

  7. Huang JW, Cunningham SD (1996) Lead phytoextraction: species variation in lead uptake and translocation. New Phytol 134:75–84

    Article  CAS  Google Scholar 

  8. Kumar NP, Dushenkov V, Motto H (1995) Raskin I. Phytoextraction: the use of plants to remove heavy metals from soils. Environ Sci Technol 29:1232–1238

    Article  CAS  Google Scholar 

  9. Lasat MM (2002) Phytoextraction of toxic metals: a review of biological mechanisms. J Environ Qual 31:109–120

    CAS  Article  Google Scholar 

  10. Little P, Martin MH (1972) A survey of zinc, lead and cadmium in soil and natural vegetation around a smelting complex. Environ Pollu 3:241–254

    Article  CAS  Google Scholar 

  11. Malone C, Koeppe DE, Miller RJ (1974) Localization of lead accumulated in corn plants. Plant Physiol 53:388–394

    CAS  Article  Google Scholar 

  12. Michael WHE, Mathias E, Andreas S (2007) Chelate assisted phytoextration of heavy metals from soil. Effect, mechanism, toxicity, and fate of chelating agents. Chemosphere 68:989–1003

    Article  CAS  Google Scholar 

  13. Nelson DW, Sommers LE (1982) Methods of soil analysis, part 2. In: Page AL et al (eds) Agronomy nonograph 9. American Society of Agronomy, Madison, WI, pp 935–951

  14. Piechalak A, Kasierska A (2000) Response of legumes to lead ion stress – III conference on trace metals’ effects on organisms and environment, Sopot, 6–8 June, pp 150 (abstracts)

  15. Piechalak A, Tomaszewska B (2002) Accumulation and detoxification of lead ion in legumes. Phytochemistry 60:153–167

    Article  CAS  Google Scholar 

  16. Seaward MRD, Richardson DHS (1990) Atmospheric sources of metal pollution and effects on vegetation. In: Shaw AJ (ed) Heavy metal tolerance in plants: evolutionary aspects. CRC Press, Florida, pp 75–92

    Google Scholar 

  17. Shaw AJ (1990) Heavy metal tolerance in plants: evolutionary aspects. CRC Press, Florida

    Google Scholar 

  18. Shivendra VS, Natalie LS (2002) Characterization of a lead hyperaccumulator shrub, Sesbania drummondii. Environ Sci Technol 36:4676–4680

    Article  Google Scholar 

  19. Wilkins DA (1957) A technique for the measurement of lead tolerance in plants. Nature 180:37–38

    Article  CAS  Google Scholar 

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The present investigation was supported by the Natural Science Foundation of Hunan (No. 04JJ3013) and the National Basic Research Programs of China (2006CB403403).

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Correspondence to Yong Li Li.

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Li, Y.L., Liu, Y.G., Liu, J.L. et al. Effects of EDTA on Lead Uptake by Typha orientalis Presl: A New Lead-Accumulating Species in Southern China. Bull Environ Contam Toxicol 81, 36–41 (2008).

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  • EDTA
  • Lead
  • Accumulator
  • Typha orientalis Presl