Skip to main content
Log in

Growth and Antioxidant System of Escherichia coli in Response to Microcystin-RR

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria. The toxic effects of microcystins on animals and plants have been well studied. However, the reports about the effects of microcystins on microbial cells are very limited. In present paper, Escherichia coli was undertaken to determine the effect of microcystin-RR. These results suggested that microcystin-RR could prolong the growth of E. coli when exposed to high concentrations of microcystin-RR and cause the accumulation of ROS and induce the oxidant stress for a short time. The antioxidant system protects E. coli from oxidative damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Beers RF, Aizer IW (1952) A spectrophotometric method for measuring breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140

    CAS  Google Scholar 

  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566. doi:10.1016/0003-2697(87)90489-1

    Article  CAS  Google Scholar 

  • Carmichael WW (1994) The toxin of cyanobacteria. Sci Am 270:64–72

    Article  Google Scholar 

  • Dixon RA, Al-Nazawi M, Alderson G (2004) Permeabilising effects of sub-inhibitory concentrations of microcystin on the growth of Escherichia coli. FEMS Microbiol Lett 230:167–170. doi:10.1016/S0378-1097(03)00910-8

    Article  CAS  Google Scholar 

  • Griffith OW (1980) Determination of glutathione and glutathione desulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207–212. doi:10.1016/0003-2697(80)90139-6

    Article  CAS  Google Scholar 

  • Harada KI, Suzuki M, Dahlem AM, Beasley VR, Carmichael WW, Rinehart KL (1988) Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon 26:433–439. doi:10.1016/0041-0101(88)90182-1

    Article  CAS  Google Scholar 

  • Hu ZQ, Liu YD, Li DH, Dauta A (2005) Growth and antioxidant system of the cyanobacterium Synechococcus elongates in response to microcystin-RR. Hydrobiologia 534:23–29. doi:10.1007/s10750-004-1319-y

    Article  CAS  Google Scholar 

  • Jones GJ, Orr PT (1994) Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay. Water Res 28:871–876. doi:10.1016/0043-1354(94)90093-0

    Article  CAS  Google Scholar 

  • Jos A, Pichardo S, Prieto A, Repetto G, Vazques CM, Moreno I, Camean AM (2005) Toxic cyanobacterial cells containing microcystins induce oxidative stress in exposed tilapia fish (Oreochromis sp.) under laboratory conditions. Aquat Toxicol 72:261–271. doi:10.1016/j.aquatox.2005.01.003

    Article  CAS  Google Scholar 

  • Li XY, Liu YD, Song LR, Liu JT (2003) Responses of antioxidant systems in the hepatocytes of common carp (Cyprinus carpio L.) to the toxicity of microcystin-LR. Toxicon 42:85–89. doi:10.1016/S0041-0101(03)00104-1

    Article  CAS  Google Scholar 

  • Lushchak V, Semchyshyn H, Lushchak O, Mandryk S (2005) Diethyldithiocarbamate inhibits in vivo Cu, Zn-superoxide dismutase and perturbs free radical processes in the yeast Saccharomyces cerevisiae cells. Biochem Biophys Res Commun 338:1739–1744. doi:10.1016/j.bbrc.2005.10.147

    Article  CAS  Google Scholar 

  • Steels EL, Learmonth RP, Watson K (1994) Stress tolerance and membrane lipid unsaturation in Saccharomyces cerevisiae grown aerobically or anaerobically. Microbiology 140:569–576

    Article  CAS  Google Scholar 

  • Yin LY, Huang JQ, Huang WM, Li DH, Wang GH, Liu YD (2005) Microcystin-RR-induced accumulation of reactive oxygen species and alteration of antioxidant systems in tobacco BY-2 cells. Toxicon 46:507–512. doi:10.1016/j.toxicon.2005.06.015

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Basic Research programs of China (2002 CB 412306, 2003CB716801) and a National Hi-Tech Project of China (2005AA601010).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Y. D. Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, C.Y., Wang, W.B., Li, D.H. et al. Growth and Antioxidant System of Escherichia coli in Response to Microcystin-RR. Bull Environ Contam Toxicol 81, 427–431 (2008). https://doi.org/10.1007/s00128-008-9436-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-008-9436-3

Keywords

Navigation