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Atrazine Exposure Decreases Cell Proliferation in Chinese Hamster Ovary (CHO-K1) Cell Line

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Abstract

The aim of this study was to determine the toxic effect of atrazine at the ovarian cellular level. Chinese Hamster Ovary (CHO-K1) cell line was used to evaluate the degree of in vitro atrazine cytotoxicity and the morphological changes were followed during the cell death. Application of four bioassays confirmed that atrazine decreases ovarian cell proliferation and IC50 were determined with each assay after 72 h of exposure. The level of apoptosis in atrazine treated cells was low.

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References

  • Cooper RL, Stoker TE, Tyrey L, Goldman JM, McElroy WK (2000) Atrazine disrupts the hypothalamic control of pituitary-ovarian function. Toxicol Sci 53:297–307

    Article  CAS  Google Scholar 

  • Crisp TM, Clegg ED, Cooper RL, Wood WP, Anderson DG, Baetcke KP (1998) Environmental endocrine disruption: an effects assessment and analysis. Environ Health Perspect 106:11–56

    Article  CAS  Google Scholar 

  • Freshney RI (2005) Culture of animal cells: a manual of basic techniques, 5th edn. John Wiley & Sons Inc., Hoboken, NJ

    Google Scholar 

  • Fujimoto N, Honda H (2003) Effects of environmental estrogenic compounds on growth of a transplanted estrogen responsive pituitary tumor cell line in rats. Food Chem Toxicol 41:1711–1717

    Article  CAS  Google Scholar 

  • Gianessi LP, Marcelli MB (2000) Pesticide use in U.S. crop production: 1997, National Summary report, USDA, Washington DC

    Google Scholar 

  • Kniewald J, Mildner P, Kniewald Z (1979) Effects of s-trazine herbicides on hormone-receptor complex formation, 5alpha-reductase and 3alpha-hydroxysteroid dehydrogenase activity at the anterior pituitary level. J Steroid Biochem 11:833–838

    Article  CAS  Google Scholar 

  • Kniewald J, Kmetič I, Gaurina Srček V, Kniewald Z (2005) Alternative models for toxicity testing of xenobiotics. Arh Hig Rada Toksikol 56:195–204

    CAS  Google Scholar 

  • Manske MK, Beltz LA, Dhanwada KR (2004) Low-level atrazine exposure decreases cell proliferation in human fibroblasts. Arch Environ Contam Toxicol 46:438–444

    Article  CAS  Google Scholar 

  • National Research Council (1999) Committee on hormonally active agents in the environment. Hormonally active agents in the environment. National Academy Press, Washington

    Google Scholar 

  • OECD (1993) Guidelines for testing of chemicals, guideline 422: combined repeated dose toxicity study with the reproduction/developmental toxicity screening test. OECD, Paris

    Google Scholar 

  • O’Hare S, Atterwill CK (eds) (1995) In vitro toxicity testing protocols, methods in molecular biology, vol 43. Humana Press, Totowa, NJ

    Google Scholar 

  • Peruzović M, Kniewald J, Čapkun V, Milković K (1995) Effect of atrazine ingested prior to mating on rat females and their offsprings. Acta Physiol Hung 83:79–89

    Google Scholar 

  • Rayburn AL, Moody DD, Freeman JL (2005) Cytotoxicity of technical grade versus formulations of atrazine and acetochlor using mammalian cells. Bull Environ Contam Toxicol 75:691–698

    Article  CAS  Google Scholar 

  • Šimić B, Kniewald J, Kniewald Z (1994) Effects of atrazine on reproductive performance in the rat. J Appl Toxicol 14:401–404

    Article  Google Scholar 

  • Stoker TE, Robinette CL, Cooper RL (1999) Maternal exposure to atrazine during lactation suppresses suckling-induced prolactin release and results in prostatitis in the adult offspring. Toxicol Sci 52:68–79

    Article  CAS  Google Scholar 

  • Stoker TE, Laws SC, Guidici DL, Cooper RL (2000) The effect of atrazine on puberty in male Wistar rats: an evaluation in the protocol for the assessment of pubertal development and thyroid function. Toxicol Sci 58:50–59

    Article  CAS  Google Scholar 

  • Težak Ž, Šimić B, Kniewald J (1992) Effect of pesticides on oestradiol-receptor complex formation in rat uterus cytosol. Food Chem Toxic 30:879–855

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by Ministry of Science, Education and Sports of the Republic of Croatia, grants no. 0582184-2232 and no. 0582184-2414.

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Authors and Affiliations

  1. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St 6, 10000, Zagreb, Croatia

    I. Kmetič, V. Gaurina Srček, I. Slivac, B. Šimić, Z. Kniewald & J. Kniewald

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  1. I. Kmetič
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  2. V. Gaurina Srček
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  3. I. Slivac
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  4. B. Šimić
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  5. Z. Kniewald
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  6. J. Kniewald
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Correspondence to J. Kniewald.

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Kmetič, I., Gaurina Srček, V., Slivac, I. et al. Atrazine Exposure Decreases Cell Proliferation in Chinese Hamster Ovary (CHO-K1) Cell Line. Bull Environ Contam Toxicol 81, 205–209 (2008). https://doi.org/10.1007/s00128-008-9425-6

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  • DOI: https://doi.org/10.1007/s00128-008-9425-6

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