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Toxicity of Anllóns River Sediment Extracts Using Microtox and the Zucconi Phytotoxicity Test


Two methods of environmental toxicological tests were compared and applied to bed sediments of the Anllóns River: the standardized toxicological test based on inhibition of luminescence employing Vibrio fischeri, and a phytotoxicity test, using garden cress (Lepidium sativum L.), water cress (Nasturtium officinalis L.) and annual rye-grass (Lolium multiflorum L.) seeds. In addition, TCLP and HCl extracts were evaluated. The inhibition of luminescence test showed more sensitivity to toxicants than phytotoxicity assays, and no significant correlations were found between them. On the other hand, TCLP metal concentrations (Fe, Al, Zn, Pb, As) were lower than HCl concentrations, but seemed to represent more accurately the phytoavailability of metals to plants.

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The present study was financed by the Science and Education Ministry of Spain, which granted Rosa Devesa with a FPI grant (MEC, REN 2003-08673/BES-2004-5894). The authors wish to thank two anonymous reviewers for their helpful comments, which substantially improved this manuscript.

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Correspondence to R. Devesa-Rey.

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Devesa-Rey, R., Moldes, A.B., Díaz-Fierros, F. et al. Toxicity of Anllóns River Sediment Extracts Using Microtox and the Zucconi Phytotoxicity Test. Bull Environ Contam Toxicol 80, 225–230 (2008).

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  • Sediments
  • Vibrio fischeri
  • Phytotoxicity
  • TCLP elutriate
  • Heavy metals