This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 μg/mL) for 0–6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-s-transferase (GST) activities significantly increased after 3–4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 μg/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.
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The present investigation was supported by the National Basic Research Programs of China (2002CB412300, 2003CB716801) and the National Hi-Tech Project (2005AA601010) and the Project of Chinese Academy of Sciences (KSCX2-1-10).
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Huang, W.M., Xing, W., Li, D.H. et al. The Role of Glutathione Metabolism in Tolerance of Tobacco BY-2 Suspension Cells to Microcystin-RR. Bull Environ Contam Toxicol 80, 215–219 (2008). https://doi.org/10.1007/s00128-007-9348-7
- Tobacco BY-2 suspension cells
- Glutathione metabolism
- Oxidative stress