Skip to main content

Genotoxicity of Acqueous Elutions of Industrial Soils


The genotoxicity of industrial soils was evaluated. A single cell electrophoresis assay or comet assay, using eleocyte cells of Eisenia foetida, was performed to assess the genotoxicity of aqueous elutions. These were obtained from industrial soils containing metals. All soil samples meet the environmental quality guidelines for metal concentrations. However, elutions have produced genotoxic effects at dilutions as low as 6%. Total metal concentrations for each aqueous elution could express synergistic effects of these compounds.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2


  1. Adamowicz A, Wojtaszek J (2001) Morphology and phagocytotic activity of coelomocytes in Dendrobaena veneta (Lumbricidae). Zool Pol 46:91–104

    Google Scholar 

  2. APHA-AWWA-WPCF (1998) Method 2540. In: Franson M (Ed.). Standard methods for the examination of water and wastewater. 20th edn. American Public Association. American Water Works. Association. Water Environment Federation, Washington

    Google Scholar 

  3. Bierkens J, Klein G, Corbisier P, Van den Heuvel R, Verschaeve L, Weltens R, Schoeters G (1998) Comparative sensitivity of 20 bioassays for soil quality. Chemosphere 37:2935–2947

    Article  CAS  Google Scholar 

  4. Carter MR (1993) Soil sampling and methods of analysis. Lewis Publishers, Boca Raton

    Google Scholar 

  5. Di Marzio WD, Saenz ME, Lemiere S, Vasseur P (2005) Improved single cell gel electrophoresis assay for the earthworm Eisenia foetida. Environ Mol Mutagen 46:246–252

    Article  CAS  Google Scholar 

  6. Eyambe GS, Goven AJ, Fitzpatrick LC, Venables BJ, Cooper EL (1991) A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies. Lab Anim 25:61–67

    Article  CAS  Google Scholar 

  7. Hartwig A (1998) Carcinogenicity of metal compounds: possible role of DNA repair inhibition. Toxicol Lett 102–103:235–239

    Article  Google Scholar 

  8. Hassoun EA, Stohs SJ (1996) Cadmium-induced production of superoxide anion and nitric oxide, DNA single strand breaks and lactate dehydrogenase leakage in J774A.1 cell cultures. Toxicology 112:219–226

    Article  CAS  Google Scholar 

  9. Lavado RS, Rodríguez MB, Taboada MA (2005) Treatment with biosolids affects soil availability and plant uptake of potentially toxic elements. Agric Ecosyst Environ 109:360–364

    Article  CAS  Google Scholar 

  10. Lee AJ, Hodges NJ, Chipman JK (2004) Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage: optimization and reproducibility. Biomarkers 9:103–115

    Article  CAS  Google Scholar 

  11. Ley 24051, (1993) Reglamentación de la Ley 24051, Decreto N° 831/93. En Boletín oficial Argentina, Residuos peligrosos, Buenos Aires

  12. Reinecke SA, Reinecke AJ (2004) The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch Environ Contam Toxicol 46:208–215

    CAS  Google Scholar 

  13. Silva-Pereira LC, Cardoso PC, Leite DS, Bahia MO, Bastos WR, Smith MA, Burbano RR (2005) Genotoxicity on human lymphocyte cultures. Braz J Med Biol Res 38:901–907

    CAS  Article  Google Scholar 

  14. USDA (1999) Soil taxonomy. 2nd edn. United States Department of Agriculture. Natural Resources Conservation Service, Washington

    Google Scholar 

  15. USDA (2003) Keys to soil taxonomy. 9th edn. United States Department of Agriculture. Natural Resources Conservation Service, Washington

    Google Scholar 

  16. WEST Inc, (1996) TOXSTAT V3.5. Western EcoSystems Technology Inc., Cheyenne, WY, USA.

  17. Wozniak K, Blasiak J (2003) In vitro genotoxicity of lead acetate: induction of single and double DNA strand breaks and DNA–protein cross-links. Mutat Res 535:127–139

    CAS  Google Scholar 

  18. Wozniak K, Blasiak J (2004) Nickel impairs the repair of UV- and MNNG damaged DNA. Cell Molec Biol Lett 9:83–94

    CAS  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to W. D. Di Marzio.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Di Marzio, W.D., Sáenz, M.E., Montivero, C. et al. Genotoxicity of Acqueous Elutions of Industrial Soils. Bull Environ Contam Toxicol 79, 483–487 (2007).

Download citation


  • Genotoxicity
  • Metals
  • Comet assay