Abstract
In this study, 14C-benzo[a]pyrene (BaP) was chosen as a model compound to investigate if photosensitization by riboflavin enhances the subsequent microbial mineralization of polycyclic aromatic hydrocarbons (PAHs) in natural aquatic environments. After photolysis, BaP showed an increased toxicity to human epithelial cell and natural microbial assemblage. However, BaP mineralization rate in a river water sample containing riboflavin is roughly twice of that without riboflavin after the 2-day incubation. Thus, the results imply that microbial assemblage can mineralize BaP photoproducts to carbon dioxide and a combination of riboflavin photosensitization and microbial degradation could lead to complete detoxification of PAHs.
Similar content being viewed by others
References
Arfsten DP, Schaeffer DJ, Mulveny DC (1996) The effects of near ultraviolet radiation on the toxic effects of polycyclic aromatic hydrocarbons in animals and plants: a review. Ecotoxicol Environ Saf 33:1–24
Baird W, Hooven L, Brinda M (2005) Carcinogenic polycyclic aromatic hydrocarbon-DNA adducts and mechanism of action. Environ Mol Mutagen 45:106–114
Hu X, Zhang Y, Zhao X, Hwang HM (2007) Cytotoxicity and genotoxicity of benzo[a]pyrene and its enzymatic degradation intermediates to HaCaT and A3 cells. Environ Mol Mutagen 48:106–113
Lampi M, Gurska J, McDonald K, Xie F, Huang XD, Dixon DG, Greenberg BM (2005) Photoinduced toxicity of polycyclic aromatic hydrocarbons to Daphnia magna: ultraviolet-mediate effects and the toxicity of polycyclic aromatic hydrocarbon photoproducts. Environ Toxicol Chem 25:1079–1087
Laupeze B, Amiot L, Sparfel L, Ferrec EL, Fauchet R, Fardel O (2002) Polycyclic aromatic hydrocarbons affect functional differentiation and maturation of human monocyte-derived dendritic cells. J Immunol 168:2652–2658
Lehto K, Puhakka J, Lemmetyinen H (2003) Photodegradation products of polycyclic aromatic hydrocarbons in water and their amenability to biodegradation. Polycycl Aromat Comp 23:401–416
Manodori L, Gambaro A, Piazza R, Ferrari S, Stortini AM, Moret I, Capodaglio G (2006) PCBs and PAHs in sea-surface microlayer and sub-surface water samples of the Venice Lagoon (Italy). Mar Pollut Bull 52:184–192
Momzikoff A, Santus R, Giraud M (1983) A study of the photosensitizing properties of seawater. Mar Chem 12:1–14
Qiao M, Wang C, Huang S, Wang D, Wang Z (2006) Composition, sources, and potential toxicological significance of PAHs in the surface sediments of the Meiliang Bay, Taihu Lake. China Environ Int 32:28–33
Shemer H, Linden KG (2007) Photolysis, oxidation and subsequent toxicity of a mixture of polycyclic aromatic hydrocarbons in natural waters. J Photochem Photobiol A Chem 187:186–195
Zeng K, Hwang HM, Zhang Y, Cook S (2004) Assessing cytotoxicity of photosensitized transformation products of 2,4,6-trinitrotoluene (TNT) and atrazine with freshwater microbial assemblages. Environ Toxicol 19:490–496
Zhao X, Hu X, Hwang HM (2006) Effects of riboflavin on the phototransformation of benzo[a]pyrene. Chemosphere 63:1116–1123
Acknowledgments
This research was supported by the following grants: (1) NIH-SCORE S06GM08047 (to JSU) and (2) U.S. Department of the Army # DAAD 19-01-1-0733 and # W911NF-04-1-0327 to JSU. This project was performed, in part using compounds provided by the National Cancer Institute’s Chemical Carcinogen Reference Standards Repository operated under contract by Midwest Research Institute, No. N02-CB-07008. Authors also appreciate Sean Cook of Department of Biology at JSU for his help in the experiment.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhao, X., Zhang, Y., Hu, X. et al. Enhanced Bio-Mineralization by Riboflavin Photosensitization and its Significance to Detoxification of Benzo[a]pyrene. Bull Environ Contam Toxicol 79, 319–322 (2007). https://doi.org/10.1007/s00128-007-9259-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-007-9259-7