Abstract
Seven strains that can degrade nitrobenzene at low temperature were isolated from the sediments of a nitrobenzene-polluted river. One of the strains, NB-1, can mineralize 20 mg/L nitrobenzene completely from 2.5 to 35°C with an optimum temperature of 25°C. NB-1 was identified as Pseudomonas putida according to its morphology, biochemical properties, and 16S rDNA sequence analysis. The strain could tolerate and degrade 120 mg/L nitrobenzene within 175 h at 5°C. It can be used for the bioremediation of nitrobenzene-contaminated environment.
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References
Haigler BE, Spain JC (1991) Biotransformation of nitrobenzene by bacteria containing toluene degradative pathways. Appl Environ Microbiol 57(11):3156–3162
Hughes JB, Beckles DM, Chandra SD, Ward CH (1997) Utilization of bioremediation processes for the treatment of PAH-contaminated sediments. J Ind Microbiol Biotechnol 18: 152–160
Lessner DJ, Parales RE, Narayan S, Gibson DT (2003) Expression of nitroarene dioxygenase genes in Comamonas sp. strain JS765 and Acidovorax sp. strain JS42 is induced by multiple aromatic compounds. J Bacteriol 185:3895–3904
Mackey QD, Shin WY, Ma KC (1995) Illustrated handbook of physical properties and environmental fate for organic chemicals. Lewis publishers, Boca Raton
Margesin R, Schinner F (1997) Bioremediation of diesel oil-contaminated Alpine soils at low temperatures. Appl Microbiol Biotechnol 47: 462–468
Morita RY (1975) Psychrophilic bacteria. Bacteriol Rev 39: 144–167
Nishino SF, Spain JC (1995) Oxidative pathway for the biodegradation of nitrobenzene by Comamonas sp. strain JS765. Appl Environ Microbiol 61:2308–2313
Philipp B, Pierre-Alain F, Nicole N, Franz S, Rosa M (2005) Biodegradation of phenol and phenol-related compounds by psychrophilic and cold-tolerant alpine yeasts. Chemosphere 59:909–918
Storck WJ, Layman PL, Reisch MS, Thayer AM (1996) Facts and figures for the chemical industry. Chem Eng News 24:38–79
www.chinaview.cn (2005) Major river pollution confirmed in northeast China, 2005–11—23,16:14:42, http://www.news.xinhuanet.com/english/2005-11/23/content_3823846.htm
Zhao JS, Owen PW (2000) Cometabolic biotransformation of nitrobenzene by 3-nitrophenol degrading Pseudomonas putida. Can J Microbiol 46(7):643–648
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Li, Y., Hu, H. & Wu, Q. Isolation and Characterization of Psychrotrophic Nitrobenzene-Degrading Strains from River Sediments. Bull Environ Contam Toxicol 79, 340–344 (2007). https://doi.org/10.1007/s00128-007-9239-y
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DOI: https://doi.org/10.1007/s00128-007-9239-y