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Isolation and Characterization of Psychrotrophic Nitrobenzene-Degrading Strains from River Sediments


Seven strains that can degrade nitrobenzene at low temperature were isolated from the sediments of a nitrobenzene-polluted river. One of the strains, NB-1, can mineralize 20 mg/L nitrobenzene completely from 2.5 to 35°C with an optimum temperature of 25°C. NB-1 was identified as Pseudomonas putida according to its morphology, biochemical properties, and 16S rDNA sequence analysis. The strain could tolerate and degrade 120 mg/L nitrobenzene within 175 h at 5°C. It can be used for the bioremediation of nitrobenzene-contaminated environment.

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Correspondence to Hongying Hu.

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Li, Y., Hu, H. & Wu, Q. Isolation and Characterization of Psychrotrophic Nitrobenzene-Degrading Strains from River Sediments. Bull Environ Contam Toxicol 79, 340–344 (2007).

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  • Nitrobenzene
  • Biodegradation
  • Psychrotrophic strain
  • Pseudomonas putida