Summary
The role of syntaxin 1, a protein involved in the docking of synaptic vesicles at presynaptic active zones, has been investigated in pancreatic islet cells. Using two different monoclonal antibodies we have shown that syntaxin 1 is present in the pancreatic islet cell microsomal fraction. Furthermore, functional experiments demonstrate that anti-syntaxin antibodies inhibit Ca2+-dependent insulin secretion in permeabilized islet cells. These data indicate that syntaxin 1 is present in the pancreatic beta cell and it is likely to play a functional role in the exocytosis of secretory granules.
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Abbreviations
- [Ca2+]i :
-
Intracellular free calcium concentration
- PMSF:
-
phenyl methyl sulphonyl fluoride
- PVDF:
-
polyvinylidene difluoride
- SNAP:
-
synaptosomal associated protein
- SNARE:
-
SNAP receptor
- VAMP:
-
vesicle-associated membrane protein
- mAb:
-
monoclonal antibody]
- clone HPC-1:
-
anti-syntaxin monoclonal antibody
References
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Martín, F., Moya, F., Gutierrez, L.M. et al. Role of syntaxin in mouse pancreatic beta cells. Diabetologia 38, 860–863 (1995). https://doi.org/10.1007/s001250050364
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DOI: https://doi.org/10.1007/s001250050364