Summary
We investigated the possible association between alleles of a simple tandem repeat DNA polymorphism in the human glycogen synthase gene and non-obese non-insulin-dependent diabetes (NIDDM) in Japanese subjects. Nine alleles (- 4G, - 3G, - 2G,- 1G, OG, 1G, 2G, 3G, and 4G) were identified in the study group of 164 patients with NIDDM and 115 non-diabetic subjects. The overall frequency distribution of the glycogen synthase gene alleles was significantly different between the two groups (p = 0.0316). The 2G al-lele was found more frequently in patients with NIDDM than in non-diabetic subjects (17.7 % vs 8.7 %, p =0.0016). These results suggest that the 2G allele could be a genetic marker of NIDDM in Japanese subjects.
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Abbreviations
- NIDDM:
-
non-insulin-dependent diabetes melli-tus
- RFLP:
-
restriction fragment length polymorphism
- PCR:
-
po-lymerase chain reaction
References
Permutt MA (1991) Use of DNA polymorphisms for genetic analysis of non-insulin dependent diabetes mellitus. In: Harrison L, Tait B (eds) Genetics of diabetes part II. Baillere Tindall/Saunders, London, pp 495–526
Groop LC, Kankuri M, Schalin-Jantti C et al. (1993) Association between polymorphism of the glycogen synthase gene and non-insulin-dependent diabetes mellitus. N Engl J Med 328: 10–14
Kadowaki T, Kadowaki H, Yazaki Y (1993) Polymorphism of the glycogen synthase gene and non-insulin-dependent diabetes mellitus. N Engl J Med 328: 1568–1569
Vionnet N, Bell GI (1993) Identification of a simple tandem repeat DNA polymorphism in the human glycogen synthase gene and linkage to five markers on chromosome 19q. Diabetes 42: 930–932
Thorburm AW, Gumbiner B, Bulacan F, Brechtel G, Henry RR (1991) Multiple defects in muscle glycogen synthase ac- tivity contribute to reduced glycogen synthesis in non-insulin dependent diabetes mellitus. J Clin Invest 87:489–495
Vaag A, Henriksen JE, Beck-Nielsen H(1992) Decreased insulin activation of glycogen synthase in skeletal muscles in young nonobese Caucasian first-degree relatives of patients with non-insulin-dependent diabetes mellitus. J Clin Invest 89:782–788
Schalin-Jantti C, Harkonen M, Groop LC (1992) Impaired activation of glycogen synthase in people at increased risk for developing NIDDM. Diabetes 41:598–604
Vestergaard H, Bjorbaek C, Andersen PH, Bak JF, Pedersen AO (1991) Impaired expression of glycogen synthase mRNA in skeletal muscle of NIDDM patients. Diabetes 40: 1740–1745
Vestergaard H, Lund S, Larsen FS, Bjerrum OJ, Pedersen O (1993) Glycogen synthase and phosphofructokinase protein and mRNA levels in skeletal muscle from insulin-resistant patients with non-insulin-dependent diabetes mellitus. J Clin Invest 91:2342–2350
Zouali H, Velho G, Froguel P (1993) Polymorphism of the glycogen synthase gene and non-insulin-dependent diabetes mellitus. N Engl J Med 328:1568
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Kuroyama, H., Sanke, T., Ohagi, S. et al. Simple tandem repeat DNA polymorphism in the human glycogen synthase gene is associated with NIDDM in Japanese subjects. Diabetologia 37, 536–539 (1994). https://doi.org/10.1007/s001250050144
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DOI: https://doi.org/10.1007/s001250050144