Summary
Recently, we demonstrated that approximately 60 % of GLUT 1 in a crude membrane fraction of rat skeletal muscle originates from perineurial sheaths. To study the in vivo regulation of GLUT 1 expression in different tissues in muscles, we measured the level of GLUT 1 in crude muscle membranes and in perineurial sheaths in diabetic (fa/fa) Zucker rats and lean controls, with and without metformin treatment. The GLUT 1 concentration in perineurial sheaths was identical in all four groups of rats, both when measured by quantitative immunofluorescence and by immunoblotting and den-sitometry. In a fraction of crude membranes of soleus muscles GLUT 1 expression was more than two-fold higher in (fa/fa) rats than in lean controls (p > 0.005). Metformin treatment significantly elevated GLUT 1 in control rats (p > 0.05) and tended to decrease GLUT 1 in diabetic rats (p > 0.075). The expressions of GLUT 1 and GLUT 4 in crude muscle membranes were inversely correlated (p > 0.01), and GLUT 1 expression correlated positively with fasting glucose (p > 0.05). In conclusion, GLUT 1 expression in perineurial sheaths is unaffected by alterations in glucose homeostasis and by the genes responsible for obesity and diabetes in the Zucker rat. GLUT 1 expression in a crude membrane fraction of soleus muscle is increased in the diabetic animals, likely due to an increased expression in muscle cells proper.
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Handberg, A., Micheelsen, J., Vinten, J. et al. Elevated GLUT 1 level in crude muscle membranes from diabetic Zucker rats despite a normal GLUT 1 level in perineurial sheaths. Diabetologia 37, 443–448 (1994). https://doi.org/10.1007/s001250050130
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DOI: https://doi.org/10.1007/s001250050130