Islet vascular fibrosis may play an important role in the progression of type 2 diabetes, but there are no mouse models allowing detailed mechanistic studies to understand how a dysfunctional islet microvasculature contributes to diabetes pathogenesis. Here we report that the transgenic AktTg mouse, unlike other mouse strains, shows an increased deposition of extracellular matrix (ECM) proteins in perivascular regions, allowing us to study the cellular mechanisms that lead to islet vascular fibrosis.
Using immunohistochemistry, we labelled the islet microvasculature and ECM in pancreas sections of AktTg mice and human donors and performed lineage tracing to follow the fate of islet pericytes. We compared islet microvascular responses in living pancreas slices from wild-type and AktTg mice.
We found that vascular pericytes proliferate extensively, convert into profibrotic myofibroblasts and substantially contribute to vascular fibrosis in the AktTg mouse model. The increased deposition of collagen I, fibronectin and periostin within the islet is associated with diminished islet perfusion as well as impaired capillary responses to noradrenaline (norepinephrine) and to high glucose in living pancreas slices.
Our study thus illustrates how the AktTg mouse serves to elucidate a cellular mechanism in the development of islet vascular fibrosis, namely a change in pericyte phenotype that leads to vascular dysfunction. Because beta cells in the AktTg mouse are more numerous and larger, and secrete more insulin, in future studies we will test the role beta cell secretory products play in determining the phenotype of pericytes and other cells residing in the islet microenvironment under physiological and pathophysiological conditions.
Pancreatic islets are highly vascularised endocrine mini-organs that regulate glucose homeostasis . Their dense network of blood vessels and extracellular matrix (ECM) are essential to maintain endocrine cell survival and function . Islets in people with type 2 diabetes are characterised by vascular fibrosis consisting of an excessive deposition of ECM proteins around islet blood vessels . Although it is not central to current models of diabetes pathogenesis , earlier studies suggested that islet vascular fibrosis plays an important role in the pathogenesis of type 2 diabetes . Vascular fibrosis could lead to defective hormone secretion either by causing cytoarchitectural defects and reducing beta cell mass [6,7,8,9,10] or by compromising microvascular function, causing vascular leakage  and interfering with exchanges between endocrine cells and the blood [12, 13]. Little is known, however, about the specific cellular and molecular mechanisms that promote islet vascular fibrosis in type 2 diabetes. Unfortunately, there is a lack of adequate model organisms allowing detailed mechanistic studies to understand how a dysfunctional islet microvasculature contributes to diabetes pathogenesis.
The mouse is widely used to study islet biology and diabetes pathogenesis because it can be manipulated to establish causal relationships. However, important aspects of the microvasculature and the local extracellular microenvironment of the human islet are not modelled in the mouse. The human islet has fewer and shorter blood vessels, with smaller vessel diameters and reduced vessel branching than mouse islets [14, 15]. There are major differences in the composition of the basement membrane , including a dense accumulation of collagen around the microvasculature in the human islet [; Fig. 1). A caveat to these comparisons is that they are made mostly to the C57BL/6 mouse, which may not be the best choice to study islet vascular biology and the local microenvironment. We searched for a mouse model for studying pathophysiological changes in the islet microvasculature and found the AktTg mouse: a transgenic mouse model of beta cell expansion, whose beta cell mass is significantly higher than in wild-type animals owing to enhanced beta cell hyperplasia and hypertrophy . Importantly, islets from these mice showed an increased deposition of ECM proteins in perivascular regions, allowing us to study cellular mechanisms leading to vascular fibrosis in the islet.
Given their pluripotent and postnatal undifferentiated nature, vascular pericytes can adopt various phenotypes. For instance, pericytes can convert into myofibroblasts in fibrotic diseases in different tissues [19,20,21,22,23]. Pericytes at the islet border (endocrine–exocrine interface) have been shown to adopt a myofibroblast-like appearance in rat models of type 2 diabetes and hypertension [13, 24]. We therefore hypothesised that, in the AktTg mouse, pericytes contribute to vascular fibrosis by becoming profibrotic myofibroblasts. We used cell lineage tracing to follow the fate of islet pericytes and assessed the functional consequences of the anatomical alterations of the islet microvasculature using living mouse pancreas slices.
Detailed descriptions of the experimental procedures can be found in the electronic supplementary material (ESM) Methods.
All the experiments were performed with 3–4 month old female and male mice, on a C57BL/6 background. AktTg mice were generated as previously described and bred in house . All other mouse lines were from the Jackson Laboratory (USA). For lineage tracing experiments, we crossed male mice that expressed the fluorescent reporter tdTomato (Rosa26-tdTomato, stock no. 012567) with female mice that express Cre recombinase under the Cspg4 promoter (NG2-Cre, stock no. 008533; note that Cspg4 encodes neuron–glial antigen 2 [NG2], which is also known as chondroitin sulphate proteoglycan 4). Cre-positive tdTomato-positive female mice were crossed with AktTg male mice to yield NG2-tdTomato-AktTg mice. Mice harbouring a spontaneous mutation in the leptin receptor (db/db mice; stock no. 000697) were also used. To label proliferating cells, BrdU (1 mg/ml) was added to the drinking water for 21 days. All experiments were conducted according to protocols approved by the University of Miami Institutional Animal Care and Use Committee.
Human pancreas tissue sections were obtained from the Histology Core of the Diabetes Research Institute of the University of Miami. Information about human organ donors is included in ESM Table 1. Experiments were approved by the responsible ethics committee of the University of Miami.
Picrosirius red staining
Picrosirius red stain in combination with polarisation microscopy was used to visualise collagen in tissue sections . Pancreatic sections (10 μm) from wild-type, db/db and AktTg mice and human donors were deparaffinised in xylene and rehydrated in ethanol series before incubation with picrosirius red solution (0.5 g of Direct red 80 in 500 ml of saturated aqueous solution of picric acid) for 60 min. The sections were washed twice with acidified water (5 ml acetic acid (glacial) in 1 litre water), dehydrated and mounted. Slides were observed under a microscope (BX41, Olympus, USA) equipped with polarised light and images analysed with ImageJ software (http://imagej.nih.gov/ij/). The density of picrosirius red staining was calculated by dividing the area of red-stained structures within the islet parenchyma by the islet area. Quantification was performed in a blind fashion.
Preparation of living pancreatic slices
Pancreatic slices (150 μm thickness) were prepared from wild-type and AktTg mice (3–4 months old) as previously described . Slices were incubated in HEPES-buffered solution containing 3 mmol/l glucose. For blood vessel labelling, Lycopersicon esculentum lectin conjugated to DyLight 594 (DL-1177, Vector Labs, USA) was injected (75 μg) in the tail vein 10 min before sacrificing.
Confocal imaging of living pancreatic slices
Living pancreatic slices of wild-type and AktTg mice were imaged on a Leica SP5 upright confocal microscope (Leica Microsystems, Germany) using a ×40 water immersion objective . Slices were continuously perfused with HEPES-buffered solution containing 3 mmol/l glucose. DyLight 594-labelled tomato lectin was excited at 594 nm and emission detected at 610–650 nm. Backscatter light was collected upon excitation with the 633 nm laser. We recorded changes in blood vessel diameter induced by noradrenaline (norepinephrine) (20 μmol/l), basal glucose solution (3 mmol/l), or by high glucose (16 mmol/l). To estimate the percentage of the islet microvasculature that was functional, we used ImageJ to quantify, in maximal projections of the islet, the area labelled with the lectin and divided it by the total islet blood vessel area (immunostained with an antibody against CD31). Quantification of islet blood vessel diameter was done as previously described . Briefly, in confocal images of the islet, we drew a straight line transversal to the blood vessel borders and used the ‘reslice’ z-function in ImageJ to generate a single image showing the changes in vessel diameter over time . We drew another line on the resliced image and, using the ‘plot profile’ function, we determined the position of the pixels with the highest fluorescence intensity and considered these the vessel borders. Vessel diameter was calculated by subtracting these two position values. Only one islet was imaged in each slice.
In this study we used 3–4 month old wild-type and AktTg mice of both sexes (n = 3). Mice were perfused with 4% PFA and the pancreases collected in tubes containing 4% PFA. Small pieces of human pancreatic tissue were fixed overnight with 4% PFA. Tissues were cryoprotected in a sucrose gradient and frozen in optimal cutting temperature compound (OCT) before cryosectioning. Immunohistochemistry was performed as previously described  and antibodies used can be found in the ESM Methods. We used ImageJ to estimate in confocal planes the numbers of pericytes and endothelial cells by manually counting the number of nuclei surrounded by cytoplasmic NG2 or CD31 immunostaining, divided by the islet area and multiplied by a mean area of 10,000 μm2. To determine vessel and ECM protein densities, in maximal projection images, we divided total immunostained area by the islet area (visualised with insulin staining). To determine colocalisation between tdTomato (red channel) and pericytes, myofibroblast markers and ECM proteins (green channel), we used the ImageJ plugin ‘intensity correlation analysis’ and calculated Mander’s colocalisation coefficients (M1 and M2). Quantifications were performed in a blind fashion.
Quantitative real-time PCR
Pancreatic islets were isolated from NG2-tdTomato wild-type mice (~7 months old) and viable tdTomato-positive and negative cells were FACS sorted from cell suspensions. Quantitative real-time PCR was performed as described in the ESM Methods to determine the expression of the following genes: Rn18s, Cspg4 (which encodes NG2), Acta2, Pdgfrb.
We used GraphPad Prism 5.0 and performed Student’s t test, one-sample t test or one-way ANOVA followed by a Tukey’s multiple comparison test. We considered statistical significance when p < 0.05. All data were assessed to ensure normal distribution and equal variance between groups. Throughout the manuscript we present data as mean ± SEM, unless otherwise stated in the figure legend.
A mouse model of islet vascular fibrosis
The microvasculature of the human islet differs from that of the mouse islet [14, 15]. In particular, the human islet is characterised by an accumulation of collagen fibres around its blood vessels [16, 17]. When we used the picrosirius red dye to specifically label collagen in pancreas sections, we found that the human islet microvasculature was outlined by the red staining (Fig. 1). Strikingly, already at a young age (17 years), ~10% of the human islet area contained picrosirius red-labelled connective tissue, a percentage that did not significantly increase with age (Fig. 1a,e). The islet microvasculature of wild-type C57BL/6 mouse, by contrast, was not stained at all (Fig. 1b,f). There were picrosirius red-labelled structures outside the mouse islet (e.g. connective tissue around acini, ducts and major blood vessels; Fig. 1b). Older C57BL/6 mice and db/db mice also did not show signs of islet vascular fibrosis (Fig. 1c). When we inspected the pancreas of the AktTg mouse, however, we found that there was intense picrosirius red labelling within the islet parenchyma, that appeared mostly as bright yellow or orange fibres upon polarised light (Fig. 1d,f and ESM Fig. 1).
The AktTg mouse is a transgenic mouse model of beta cell expansion . This mouse has increased Akt activity in beta cells due to the expression of a constitutively active Akt1 (PKB α) mutant under the control of the insulin gene promoter. Beta cell mass is significantly greater than in wild-type animals owing to enhanced beta cell proliferation and hypertrophy, yielding much higher fasting and fed plasma insulin levels and improved glucose tolerance, while maintaining normoglycaemia (, ESM Fig. 2). In this context of beta cell expansion, we not only detected abundant vascular fibrosis with picrosirius red in AktTg islets (Fig. 1d,f), but also found increased type I collagen and fibronectin immunostaining within the islet (Fig. 1g-j). Laminin density was not different between islets from wild-type C57BL/6 mice and those from AktTg mice. Perivascular regions in AktTg islets were further labelled for periostin, a myofibroblast marker and a secreted ECM protein that is involved in cellular adhesion and organisation of collagen  (Fig. 1h,j). Therefore, we took advantage of these changes and used the AktTg mouse to study pathophysiological mechanisms associated with islet vascular fibrosis.
Altered islet microvasculature in islets from AktTg mice
In view of the increased islet vascular fibrosis in the AktTg mouse model, we then examined the islet microvasculature and its cellular components: endothelial cells and pericytes. Pericytes were immunostained with two bona fide pericyte markers, NG2 and platelet-derived growth factor receptor-β (PDGFRβ) . Endothelial cells were either labelled with a fluorescent lectin from Lycopersicon esculentum (Fig. 2a) or immunostained with an antibody against the endothelial cell marker CD31 (also known as platelet endothelial cell adhesion molecule [PECAM]; Fig. 2b). We determined major alterations of the islet capillary network and pericyte coverage in AktTg mice (Fig. 2). While in wild-type mice the islet capillary network was more dense than in the surrounding exocrine tissue (; Fig. 2a,c), islets from AktTg mice exhibited fewer blood vessels (Fig. 2a,c). The typical tube morphology of capillaries made of a layer of endothelial cells covered with pericytes was disrupted in AktTg islets (Fig. 2b). Blood vessels in AktTg islets had significantly more pericytes and endothelial cells (Fig. 2b–e). Wild-type islets displayed a 1:3 ratio of pericytes:endothelial cells, consistent with our previously reported ratio in mouse pancreatic islets (; Fig. 2f). In contrast, AktTg islets showed a higher pericyte:endothelial cell ratio (~ 1:2; Fig. 2f). The morphology of blood vessels in the acinar tissue surrounding islets of AktTg mice was conserved (Fig. 2a,b). These results indicate that in the AktTg mouse the increased fibrosis is associated with changes in the structure and composition of the islet microvasculature.
Pericytes are activated and proliferate in islets from AktTg mice
To assess the contribution of proliferation to the increase in endothelial cell and pericyte numbers, we performed BrdU experiments. Consistent with published data , higher beta cell proliferation was observed in AktTg transgenic mice (ESM Fig. 2). Interestingly, approximately 60% of NG2-positive islet pericytes incorporated BrdU in AktTg mice, suggesting that the increase in pericyte number in AktTg islets was due to increased proliferation (Fig. 3a,d). The percentage of proliferating NG2-positive pericytes in AktTg mice was significantly higher than that of all the other cell populations analysed (Fig. 3d): insulin-positive beta cells, CD31-positive endothelial cells, α smooth muscle actin (αSMA)-positive cells (smooth muscle, myofibroblasts, pancreatic stellate cells; ), PDGFRβ-positive pericytes/fibroblasts (Fig. 3b,d). There were also more proliferating PDGFRβ-positive pericytes in AktTg than in wild-type mouse islets (Figs 2g, 3c,d and ESM Fig. 3).
We then assessed the magnitude of activation of critical pathways known to promote growth and proliferation: the Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signalling pathways. We used antibodies that recognise phosphorylated (active) Akt (pAkt, Ser 473) and phospho-p44/42 MAPK (ERK1/2) (Thr 202/Tyr 204). While low levels of pAkt were detected in pancreatic sections from wild-type mice, strong pAkt immunoreactivity was observed in AktTg islets (ESM Fig. 4). In AktTg islets, pAkt was detected at higher levels in endocrine cells. pAkt fluorescence intensity was also significantly higher in pericytes from AktTg islets than in pericytes in wild-type islets. pERK fluorescence could be detected in endocrine and vascular cells in wild-type islets, but its intensity significantly decreased in endocrine cells while remaining high in pericytes in islets from AktTg mice (ESM Fig. 4). Indeed, islet pericytes and exocrine tissue exhibited the highest levels of pERK immunoreactivity in pancreatic sections from AktTg mice.
A subset of pericytes in AktTg islets differentiates into myofibroblasts
Pericytes are critical for microvascular homeostasis but, owing to their pluripotent and undifferentiated nature, pericytes can differentiate into myofibroblasts in progressive fibrotic diseases [19,20,21,22]. Myofibroblasts are the major source of extracellular matrix proteins that accumulate in tissue fibrosis. To determine if islet pericytes contribute to vascular fibrosis in AktTg islets, we first used immunostaining to assess the localisation of pericytes in relation to those of the matrix proteins collagen type I and periostin (Fig. 4). Extracellular collagen type I deposits around the microvasculature in AktTg islets were always found in close association with NG2-labelled pericytes, whose cytoplasmic processes seemed to wrap and surround these ECM aggregates (Fig. 4b–e). Pericytes labelled for the myofibroblast marker periostin were found in close association with this ECM protein (Fig. 4d). These data suggest that islet pericytes could contribute to the pool of profibrotic myofibroblasts in AktTg mice. Interestingly, pericytes in islets in pancreas sections from young non-diabetic individuals also express periostin and associate closely with collagen type I (ESM Fig. 5).
To determine the potential contribution of the pericyte population to the pool of profibrotic myofibroblasts, we performed lineage tracing studies by crossing a fluorescent reporter tdTomato mouse to mice expressing Cre recombinase under the Cspg4 promoter (NG2-tdTomato mice). In these mice, vascular smooth muscle cells and pericytes express the reporter in peripheral tissues such as pancreatic islets (Fig. 5e and ESM Fig. 6; ). To validate the specificity of our Cre model, we sorted tdTomato-positive cells from NG2-tdTomato mice. As an internal control, we also collected tdTomato-negative cells. tdTomato-positive cells were indeed pericytes as shown by the increased levels of pericyte genes Cspg4 (which encodes NG2), Pdgfrb and Acta2 (which encodes αSMA) in these cells compared with tdTomato-negative cells (ESM Fig. 6).
To study changes in the pericyte phenotype in the AktTg mouse, NG2-tdTomato mice were transferred to the AktTg background to yield NG2-tdTomato-AktTg mice. NG2-tdTomato-AktTg mice showed higher plasma insulin levels, hypercellularity of vascular cells (ESM Fig. 6) and excessive perivascular ECM deposition typical of the AktTg background. Approximately 90% of tdTomato-positive cells were NG2-positive or PDGFRβ-positive mural cells in wild-type and AktTg islets, further confirming the specificity of the NG2-Cre model (Fig. 5e and ESM Fig. 6). A subset of lineage-traced tdTomato-positive cells was also labelled for αSMA, fibronectin and collagen type I in both wild-type and AktTg mice (Fig. 5c,e). Within the lineage-traced population of pericytes in islets from AktTg mice we found significant increases in the incidence of fibronectin labelled cells as well as de novo expression of fibroblast markers vimentin and periostin (Fig. 5a–e). These results indicate that pericytes give rise to profibrotic myofibroblasts in AktTg islets.
Myofibroblasts are a heterogenous cell population with different cellular origins. Of the periostin- and αSMA-positive cell populations in the islets of AktTg mice, 69 ± 3% and 40 ± 3% were tdTomato-positive, respectively. These data suggest that a subset of islet myofibroblasts (~30–60%) may originate from cell populations other than pericytes, such as pancreatic stellate cells . Nevertheless, islet pericytes contribute a substantial fraction of islet myofibroblasts in AktTg mice.
Impaired vascular perfusion and microvessel responses in AktTg islets ex vivo
To examine the functional consequences of the changes in islet pericytes and ECM in the AktTg model, we adapted the pancreatic slice technique to examine in situ responses of the fibrotic microvessels in their native environment [26, 32]. In living pancreatic tissue slices, the different tissue components are preserved allowing the study of interactions between endocrine cells, vascular cells, fibroblasts and other cell compartments. To assess the functionality of the islet microvasculature, we labelled blood vessels with an intravenous injection of a fluorescent lectin from Lycopersicon esculentum (Fig. 6a). There was a ~60% reduction in perfusion of AktTg islet, calculated as the percentage of the islet blood vessel area that was labelled with the lectin (functional vasculature; Fig. 6b). We then assessed whether the abnormalities in perfusion were associated with impaired responses to known capillary vasodilators and vasoconstrictors. In line with our published results , a subset of capillaries in wild-type mouse islets constricted upon noradrenaline stimulation (20 μmol/l; approximately 30% decrease in diameter) and dilated upon beta cell stimulation with high glucose (16 mmol/l; approximately 15% increase in diameter; Fig. 6c–e). Noradrenaline also elicited a powerful constriction of the feeding arteriole in wild-type islets (Fig. 6d, dark grey trace). In contrast, capillaries in AktTg islets were neither responsive to the vasoconstrictor noradrenaline nor to high glucose (Fig. 6c–e). Lack of responses of AktTg islet capillaries was not due to poor tissue viability as endocrine cells in AktTg islets still responded to KCl depolarisation (ESM Fig. 7). These data indicate that phenotypical alterations of pericytes and increased ECM accumulation around AktTg islet capillaries impair microvascular function. Islet microvascular dysfunction could be the cause of impaired insulin secretion per beta cell unit of AktTg mice in response to glucose in vivo (ESM Fig. 2d, ).
Our results show that the AktTg mouse had substantial alterations of the islet microvasculature and ECM. In particular, blood vessel density in AktTg islets was lower than in wild-type mouse islets and the number of pericytes increased 2.5-fold, yielding a higher pericyte:endothelial cell ratio. In addition, the density of different ECM proteins in perivascular regions was significantly greater in AktTg islets, and a subset of islet pericytes converted into myofibroblasts and participated in increased ECM synthesis in this transgenic mouse model.
Our lineage tracing data show that pericytes in the islet parenchyma are capable of differentiating into myofibroblasts and are actively involved in ECM synthesis, similar to their role in fibrotic diseases in different tissues [19,20,21,22,23]. Our results are in line with a previous study in a rat model of type 2 diabetes, the human islet amyloid polypeptide rat, where a pericyte synthesising fibrillar collagen was observed at the interface between the endocrine and exocrine pancreas . Interestingly, only a subset (approximately 40%) of islet pericytes adopted a myofibroblast-like phenotype, consistent with the notion that pericytes are a very heterogeneous cell population . Future studies will focus on identifying the cellular and molecular mechanisms that promote the expansion and differentiation of individual subsets of pericytes. Differential interaction with resident macrophages or with other recruited immune cells could determine whether or not they adopt a profibrotic phenotype [35, 36].
Given the higher beta cell mass and secretory activity, it is likely that islet pericytes in AktTg mice are abnormally exposed to beta cell secretory products that could affect their phenotype. Insulin, for instance, is a potent mitogenic peptide that controls cellular growth, proliferation, differentiation and migration. Insulin has been shown to stimulate the proliferation and growth of endothelial cells and pericytes in the retina [37, 38]. In addition, insulin also stimulates the proliferation of pancreatic stellate cells and the production of ECM proteins through a robust and sustained activation of Akt/mammalian target of rapamycin (mTOR)  or angiotensin II type 2 signalling pathways . In our study we observed activation of Akt and MAPK/ERK signalling pathways in pericytes in AktTg islets (ESM Fig. 4). Both pathways are downstream of the insulin receptor, suggesting that pericytes could indeed be responding to higher levels of insulin. Future studies will determine whether insulin receptor activation is involved in the phenotypical change of the subset of islet pericytes that converts into myofibroblasts.
Excessive beta cell secretory activity could affect pericytes and the ECM by additional indirect mechanisms. Several beta cell secretory products are highly proinflammatory and could potentially activate myofibroblasts [41, 42]. For instance, ATP that is co-released with insulin is a strong activator of islet and acinar macrophages . In addition, vascular endothelial growth factor A (VEGFA) released by beta cells leads to endothelial cell proliferation, thickening of the basement membrane of islet blood vessels, progressive macrophage infiltration, and proinflammatory cytokine production . The potentially inflammatory environment in AktTg islets could also activate other subpopulations of myofibroblasts not derived from pericytes, such as pancreatic stellate cells . These myofibroblast-like cells are usually found in the exocrine pancreas but are also present in pancreatic islets and their numbers increase in type 2 diabetic rats .
Endocrine cells in pancreatic islets depend on their blood vessels for proper function, and defects in the islet microvasculature can lead to diabetic phenotypes  However, because longitudinal and interventional studies are not possible in human beings, it is not known what happens at this vascular niche during the progression of type 2 diabetes. For instance, excessive deposition of ECM proteins around islet blood vessels has been described as the most frequent lesion in the islets of people with type 2 diabetes [3, 5] but neither the causes nor the functional consequences of islet vascular fibrosis have been fully elucidated. It has been suggested that the excessive deposition of connective tissue in perivascular regions in the islets could compromise capillary responses and the exchanges between beta cells and the circulation resulting in defective hormone secretion [12, 13]. Here we show that changes in pericyte coverage and perivascular fibrosis in AktTg islets lower the percentage of functional microvasculature and impair capillary responses to noradrenaline and glucose. These alterations have functional consequences. Indeed, when corrected for the significant increase in beta cell mass in AktTg mice, in vivo insulin secretion in response to glucose is impaired in transgenic animals (ESM Fig. 2d). Similar findings were previously reported by Tuttle and colleagues when they measured insulin secretion from in situ perfused pancreases of AktTg mice . These data support a potential negative impact of islet vascular fibrosis and chronic microvascular dysfunction on beta cell function. However, how acute, dynamic changes of islet microvascular function and blood flow impact hormone secretion still remains to be determined.
It is now clear that the function of pericytes goes beyond their role as mural cells of the microcirculation. Given their location at the interface between the blood and the islet parenchyma, pericytes are in a unique position to coordinate changes in the microenvironment with islet blood flow and perfusion and, ultimately, modulate islet hormone secretion. Pericytes can directly control islet perfusion and blood vessel function by actively modulating islet capillary diameter , or indirectly, by acquiring a myofibroblast-like phenotype and contributing to ECM synthesis as we show here. Excessive perivascular accumulation of ECM can result in deranged architecture and islet cell death, as well as impair microvascular function, leading, ultimately, to islet dysfunction. Using the AktTg mouse model, we now can conduct studies aimed at elucidating the role of insulin or other beta cell secretory products in determining the number, phenotype and function of islet pericytes. Elucidating the crosstalk between pericytes and beta cells is necessary to fully understand the pathogenesis of islet adaptation in diabetes.
Data presented in this manuscript are available upon request from the authors.
α Smooth muscle actin
- AktTg :
Transgenic mice expressing constitutively active Akt1 in beta cells
Extracellular signal-regulated kinase
Mitogen-activated protein kinase
Neuron–glial antigen 2
Platelet-derived growth factor receptor-β
Platelet endothelial cell adhesion molecule
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The authors thank R. Barro Soria and A. Caicedo for carefully reviewing the manuscript, K. Johnson for histological work, J. Camperio and M. Canales for data quantification, and O. Umland for helping with FACS sorting pericytes (University of Miami).
This work was funded by NIH grants K01DK111757 (JA), R01DK073716 (EBM) and DK084236 (EBM), as well as by the NIDDK-supported Human Islet Research Network (HIRN, RRID:SCR_014393; https://hirnetwork.org; UC4 DK104162, New Investigator Pilot Award to JA).
The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
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Mateus Gonçalves, L., Pereira, E., Werneck de Castro, J.P. et al. Islet pericytes convert into profibrotic myofibroblasts in a mouse model of islet vascular fibrosis. Diabetologia 63, 1564–1575 (2020). https://doi.org/10.1007/s00125-020-05168-7
- Extracellular matrix
- Pancreatic islet