Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes
Increased lipid supply causes beta cell death, which may contribute to reduced beta cell mass in type 2 diabetes. We investigated whether endoplasmic reticulum (ER) stress is necessary for lipid-induced apoptosis in beta cells and also whether ER stress is present in islets of an animal model of diabetes and of humans with type 2 diabetes.
Expression of genes involved in ER stress was evaluated in insulin-secreting MIN6 cells exposed to elevated lipids, in islets isolated from db/db mice and in pancreas sections of humans with type 2 diabetes. Overproduction of the ER chaperone heat shock 70 kDa protein 5 (HSPA5, previously known as immunoglobulin heavy chain binding protein [BIP]) was performed to assess whether attenuation of ER stress affected lipid-induced apoptosis.
We demonstrated that the pro-apoptotic fatty acid palmitate triggers a comprehensive ER stress response in MIN6 cells, which was virtually absent using non-apoptotic fatty acid oleate. Time-dependent increases in mRNA levels for activating transcription factor 4 (Atf4), DNA-damage inducible transcript 3 (Ddit3, previously known as C/EBP homologous protein [Chop]) and DnaJ homologue (HSP40) C3 (Dnajc3, previously known as p58) correlated with increased apoptosis in palmitate- but not in oleate-treated MIN6 cells. Attenuation of ER stress by overproduction of HSPA5 in MIN6 cells significantly protected against lipid-induced apoptosis. In islets of db/db mice, a variety of marker genes of ER stress were also upregulated. Increased processing (activation) of X-box binding protein 1 (Xbp1) mRNA was also observed, confirming the existence of ER stress. Finally, we observed increased islet protein production of HSPA5, DDIT3, DNAJC3 and BCL2-associated X protein in human pancreas sections of type 2 diabetes subjects.
Our results provide evidence that ER stress occurs in type 2 diabetes and is required for aspects of the underlying beta cell failure.
KeywordsApoptosis Endoplasmic reticulum stress Fatty acids Islets Pancreatic beta cells Type 2 diabetes
activating transcription factor
BCL2-associated X protein
B-cell CLL/lymphoma 2
cAMP responsive element binding protein-like 1
DNA-damage inducible transcript 3
DnaJ (Hsp40) homologue C3
eukaryotic translation initiation factor 2A
eukaryotic translation initiation factor kinase 2-alpha kinase 3
green fluorescent protein
heat shock 70 kDa protein 5
inositol requiring enzyme 1
mitogen-activated protein kinase 8
protein disulfide isomerase A4
unfolded protein response
X-box binding protein 1
Type 2 diabetes results from the failure of pancreatic beta cells to adequately compensate for obesity and insulin resistance. Both functional defects and reduced beta cell mass contribute to beta cell failure in type 2 diabetes, with apoptosis constituting the main form of beta cell death [1, 2, 3, 4]. Increased lipids and hyperglycaemia are likely causes of beta cell apoptosis [3, 4, 5, 6], but the mechanisms responsible remain unknown.
Pancreatic beta cells possess a highly developed endoplasmic reticulum (ER), required for the folding, export and processing of newly synthesised insulin [7, 8]. Various conditions that disrupt ER function, termed ER stress, lead to the accumulation of misfolded proteins in the ER [7, 8, 9]. This triggers an adaptive programme comprising four distinct responses: (1) translational attenuation, which reduces synthesis of new protein and prevents further accumulation of unfolded proteins; (2) upregulation of the genes encoding ER chaperone proteins to increase protein folding activity and to prevent protein aggregation; (3) proteosomal degradation of misfolded proteins following their regulated extrusion from the ER; and (4) apoptosis in the event of persistent stress. The signalling pathways that underlie this programme and relay information from the ER to the nucleus are known as the unfolded protein response (UPR). Heat shock 70 kDa protein 5 (HSPA5, previously known as immunoglobulin heavy chain binding protein [BIP]) is central to this process as it serves both as an ER chaperone and a sensor of protein misfolding . In non-stressed cells, HSPA5 associates on the ER luminal surface with three UPR transducer proteins, inositol requiring enzyme 1 (IRE1), activating transcription factor (ATF) 6 and eukaryotic translation initiation factor kinase 2-alpha kinase 3 (EIF2AK3, formerly known as PKR-like ER kinase [PERK]), thus maintaining them in inactive conformations. Under stressed conditions, HSPA5 dissociates from the transducer proteins, inducing their activation and subsequent upregulation of UPR target genes, as well as translational attenuation due to phosphorylation of the eukaryotic translation initiation factor 2A (EIF2A) by the protein kinase EIF2AK3. EIF2A, however, is also a substrate for other protein kinases activated during the so-called integrated stress response. When ER function is severely impaired, apoptosis is induced by enhanced transcription of DNA-damage inducible transcript 3 (DDIT3, previously known as C/EBP homologous protein [CHOP]) [11, 12] and by activation of mitogen-activated protein kinase 8 (MAPK8, formerly known as JNK1) and caspase-12 [7, 8, 9].
Using Eif2ak3-deficient mice , and in mice with a mutation in the EIF2A phosphorylation site (Ser51Ala) [14, 15], it has been demonstrated that beta cells are particularly sensitive to ER stress-induced dysfunction and death. Furthermore, studies in the Akita mouse have shown that ER stress, secondary to misfolding of mutated insulin, leads to beta cell death and glucose intolerance . Recent studies have also shown that pre-treatment of INS-1 beta cells with fatty acid leads to increased expression of several genes involved in ER stress [16, 17]. By comprehensive profiling of gene expression, we now demonstrate that saturated fatty acid induces an extensive ER stress response in MIN6 cells and that this is required for the accompanying apoptosis. Furthermore, we provide the first evidence of significant ER stress gene activation in pancreatic islets of diabetic db/db mice and humans with type 2 diabetes.
Materials and methods
Cell culture and treatment
MIN6 cells were passaged in 150 cm2 flasks with 25 ml DMEM (Invitrogen, Carlsbad, CA, USA) containing 25 mmol/l glucose, 10 mmol/l HEPES, 10% FCS, 50 U/ml penicillin and 50 μg/ml streptomycin. Cells were seeded at either 1 × 106 in 3 ml of DMEM per well in a six-well plate or at 2.6 × 105 in 0.5 ml DMEM per well in a 24-well plate. After 24 h, the medium was replaced with DMEM as above but with 6 mmol/l glucose containing either BSA or BSA coupled to oleate or palmitate (1:20 coupling; DMEM, final concentration 0.4 mmol/l fatty acid; 0.92% BSA) as previously described . Apoptosis was measured with an ELISA kit (Cell Death Detection ELISA; Roche Diagnostics, Castle Hill, NSW, Australia) .
Total RNA was extracted from MIN6 cells or islets  and real-time PCR was performed using a LightCycler (Roche Diagnostics) . Standards for each transcript were prepared in a conventional PCR and purified using a PCR product purification kit (High Pure; Roche Diagnostics). The value obtained for each specific product was normalised to a control gene (cyclophilin A) and expressed as a percentage of the value in control extracts. Transcript profiling data that had been previously reported  were re-analysed here using MAS 5.0 software (Affymetrix, Santa Clara, CA, USA).
Generation of HSPA5-overproducing MIN6 cells
Murine Hspa5 cDNA was amplified by PCR, cloned into the Gateway donor vector pDONR 221 and then subcloned into the Gateway expression vector pcDNA-DEST40 (Invitrogen). Phospho-Hspa5-DEST40 or control pmaxGFP were electroporated into MIN6 cells by nucleofection (AMAXA Biosystems, Cologne, Germany) according to the manufacturers’ instructions with >70% transfection efficiency. Cells were seeded at 5 × 105 in 1 ml of DMEM per well in a 12-well plate. After 24 h, the medium was replaced with DMEM with 6 mmol/l glucose and either BSA or BSA coupled to palmitate for 48 h.
C57BL/KsJ db/db mice and their age-matched lean db/+ littermates (control) were bred inhouse using animals originally from Jackson Laboratories (Bar Harbor, ME, USA). Procedures were approved by the Garvan Institute/St Vincent’s Hospital Animal Experimentation Ethics Committee, following guidelines issued by the National Health and Medical Research Council of Australia. Non-diabetic db/+ and diabetic db/db mice aged 10 to 12 weeks were anaesthetised and their islets isolated by pancreatic digestion with liberase RI (Roche Diagnostics). Islets were further separated with a Ficoll–Paque PLUS gradient (GE Healthcare Bio-Sciences, Uppsala, Sweden) and handpicked under a stereomicroscope. RNA or protein extraction was performed immediately following islet collection.
Cell and islet extracts were separated on NuPage SDS-PAGE gels (Invitrogen) and transferred to polyvinylidine difluoride membranes. Equal loading of protein between lanes was confirmed by Coomassie staining and subsequent β-actin immunoblots. Membranes were incubated in primary antibodies diluted in 5% BSA in Tris-buffered saline with 0.05% Tween for either 1 to 2 h at room temperature or overnight at 4°C. The following antibodies were used (1:1,000 dilution unless otherwise indicated): DDIT3 (sc-575), total EIF2A (sc-11386), and X-box binding protein 1 (XBP1; sc-7160; Santa Cruz Biotechnology, Santa Cruz, CA, USA); phospho-EIF2AK3 (Thr980, 3191), phospho-EIF2A (Ser51, 9721), cleaved caspase-3 (Asp175, 9664, 1:500; Cell Signaling Technology, Danvers, MA, USA); HSPA5 (SPA-826; Stressgen, Victoria, BC, Canada); ATF6 (1:330; Alexis Biochemicals, San Diego, CA, USA); β-actin (1:5,000; Sigma, St Louis, MO, USA); and myosin (Biomedical Technologies, Stoughton, MA, USA). DNAJC3 (1:4,000) was generously provided by A. Goodman, University of Washington, WA, USA. After incubation with horseradish peroxidase-conjugated goat anti-mouse or donkey anti-rabbit antibody (1:5,000; Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for 1 h at room temperature, immunodetection was performed by chemiluminescence (PerkinElmer, Wellesley, MA, USA).
Archival formalin-fixed, paraffin-embedded tissue was collected from Westmead Hospital, St Vincent’s Hospital Campus, Concord Hospital and Royal Prince Alfred Hospital in Sydney, Australia. The tissue sources were 11 type 2 diabetes patients and 12 non-diabetic patients, as classified from medical records, and whose pancreases were resected between 1990 and 2002. Ethical clearance was obtained from the participating Institutional Ethics Committees. Pancreas tissue microarrays consisting of 2-mm diameter tissue core biopsies containing islets were constructed. Serial sections (4 μm) were dewaxed in xylene and rehydrated in a series of graded alcohols. To unmask antigens slides were boiled in Tris–EDTA (pH 8) for 25 min. Slides were stained for HSPA5, DDIT3 and DNAJC3 using the antibodies described above, and antibodies for BCL2-associated X protein (BAX; 554104, dilution 1:1,000; BD PharMingen, San Diego, CA, USA), B cell CLL/lymphoma 2 (BCL2; M0887, dilution 1:50; DakoCytomation, Glostrup, Denmark) and insulin (I2018; dilution 1:200; Sigma). The primary antibody was visualised using EnVision+System-HRP DAB (Dako). Staining was independently assessed by two observers (D.R. Laybutt and M.C. Åkerfeldt); islet immunostaining was graded as of low, moderate or high intensity.
All results are presented as means±SEM. Statistical analyses were performed using unpaired Student’s t test or one-way ANOVA.
Time-course changes in apoptosis in MIN6 cells exposed to elevated lipids
Changes, in MIN6 cells exposed to elevated lipids, of mRNA levels of genes involved in ER stress
Changes to mRNA levels of genes involved in ER stress in MIN6 cells treated for 48 h with palmitate or oleate
100 ± 6
159 ± 16*
89 ± 5
100 ± 6
136 ± 11*
79 ± 1
100 ± 4
179 ± 7***
90 ± 3
100 ± 3
191 ± 18***
105 ± 1
100 ± 2
200 ± 33*
85 ± 6*
100 ± 7
163 ± 12**
90 ± 7
H47 (Sels, Vimp)
100 ± 3
188 ± 14***
76 ± 4**
100 ± 5
128 ± 5**
80 ± 4*
Time-course changes in ER stress markers in MIN6 cells exposed to elevated lipids
In further confirmation of the mRNA data, we observed that DDIT3 protein production was increased in a time-dependent manner by palmitate, but not by oleate (Fig. 3a,f). A similar fatty acid specificity was demonstrated for EIF2A (Ser51) phosphorylation in lipid-treated MIN6 cells, as assessed using phospho-specific antibodies (Fig. 3a,e). Thus the upregulation of UPR genes in response to palmitate precedes the accompanying beta cell apoptosis (Fig. 1).
HSPA5 overproduction partially protects MIN6 cells from lipid-induced apoptosis
Changes in mRNA levels for gene stress markers of ER in islets of db/db mice
To confirm these results in an animal model of diabetes, we next examined the expression of genes encoding UPR in islets of db/db mice. These animals develop a disease resembling the common form of human type 2 diabetes whereby insulin secretory defects and beta cell depletion prevent compensation for time-dependent increases in obesity and insulin resistance [20, 31]. We found that db/db mice displayed increased body weight (obesity), hyperglycaemia and higher circulating levels of lipid (NEFA and triacylglycerol) than lean db/+ control mice (not shown).
Increased DDIT3, HSPA5 and DNAJC3 staining in islets from human type 2 diabetes subjects
Changes in mean intensity of immunostaining in islets of type 2 diabetes subjects compared with non-diabetic subjects
Non-diabetic subjects (n)
Diabetic subjects (n)
2.5 ± 0.2 (11)
1.6 ± 0.2 (10)
1.1 ± 0.2 (10)
1.8 ± 0.3 (8)
1.8 ± 0.3 (9)
2.7 ± 0.2 (9)
2.0 ± 0.3 (12)
2.8 ± 0.1 (9)
1.9 ± 0.3 (9)
2.6 ± 0.2 (8)
Because ER stress is sufficient to induce both beta cell death [12, 13, 14, 15] and peripheral insulin resistance , its relevance to type 2 diabetes has been extensively canvassed. However, it has not yet been established whether ER stress actually occurs in type 2 diabetes or whether it makes a necessary contribution to the development of the disease. Our study addresses both of these shortfalls. First, we show a broad-based ER stress response induced in palmitate-treated MIN6 cells, an in vitro model of beta cell dysfunction. Second, we show activation of genes involved in ER stress, both in islets from db/db mice and from human type 2 diabetes subjects. Third, by demonstrating that overproduction of the ER chaperone HSPA5 partially protects against the effects of palmitate, we provide the first evidence that ER stress is required for beta cell lipoapoptosis, and by extension for the development of type 2 diabetes.
A partial requirement for ER stress has been previously described for cytokine-mediated beta cell apoptosis, involving nitric oxide generation, depletion of ER Ca2+ stores and, ultimately, induction of DDIT3 [17, 36]. In contrast, the slower development of beta cell destruction in type 2 diabetes suggests a more complex aetiology. Nevertheless, it is noteworthy that ER stress appears sufficient to cause beta cell apoptosis irrespective of the manner in which that stress is triggered. Thus, in Akita mice, a folding mutation in proinsulin is responsible ; in Wolfram syndrome ER Ca2+ handling is compromised ; whereas Walcott–Rallison syndrome involves a defect in the gene encoding EIF2AK3 . These and the transgenic animal models of Eif2ak3 deletion  or ablated EIF2A phosphorylation  all result in loss of beta cell mass and pathologies akin to type 2 diabetes. Thus, ER stress is an attractive mechanism for integrating the diverse inputs of a multifactorial disease such as type 2 diabetes and channelling them into a common, defining pathology.
Type 2 diabetes is associated with obesity and elevations in circulating fatty acids. Subtle perturbations in the capacity of pancreatic beta cells to cope with this enhanced fatty acid supply are therefore hypothesised to contribute to the onset of the disease [5, 6, 22]. In vitro, chronic fatty acid treatment of beta cells is sufficient to recapitulate both the secretory defects and apoptosis observed in type 2 diabetes [6, 18, 19]. In vivo, and over the longer term, adaptive changes might tend to protect the beta cell from chronic lipid exposure, except perhaps in the presence of predisposing defects in lipid handling or of the contribution of an additional parameter such as hyperglycaemia. Nevertheless, the in vitro models have helped define the downstream consequences of those predisposing metabolic alterations. Indeed, we show here that an extensive ER stress response must be included amongst those consequences. Importantly, we have also linked ER stress selectively to lipoapoptosis. This is based on the observation that the saturated fatty acid palmitate, but not the unsaturated fatty acid oleate, induced genes involved in ER stress and triggered apoptosis. Most importantly, lipotoxicity was significantly reduced by overproduction of HSPA5, the protein chaperone and key regulator of the UPR. This provides definitive evidence that ER stress is actually required for mediating beta cell apoptosis in response to fatty acid, thus suggesting a causal link between ER stress and aspects of beta cell dysfunction that are relevant to type 2 diabetes.
The differential cytotoxicity of saturated and unsaturated fatty acids has been extensively reported using beta cells [6, 23, 24, 25, 26], although not in one study, where both oleate and palmitate triggered ER stress in INS-1E cells . This discrepancy might relate to dosage effects since we employed fatty acids pre-coupled to BSA in a 3:1 ratio, within the physiologically elevated concentration range, as opposed to a concentrated fatty acid bolus dissolved in ethanol. Although other workers have more recently demonstrated selectivity for palmitate vs oleate in triggering ER stress and beta cell apoptosis , they have reported neither activation of all three ER stress sensors, nor a widespread elevation of UPR gene products. This might reflect subtle but important differences in experimental design. MIN6 cells are more highly differentiated than the rat INS-1 cells employed in the previous paper. Moreover, Karaskov et al. used serum-starved cells, a higher molar ratio of fatty acid to BSA (>6:1) and conducted their experiments at an elevated glucose concentration, known to exacerbate lipotoxicity [39, 40]. Under their conditions other apoptotic pathways might outstrip ER stress and perhaps curtail its full development. We employed a milder but longer exposure to fatty acids as more representative of chronic in vivo stress. We believe this approach is validated by the generally good concordance between our cell-based studies and the db/db islet data.
The triggering of ER stress by palmitate is probably not due to nitric oxide generation, which is not apparent in pure beta cell populations such as MIN6 cells [19, 23]. Neither is deposition of the saturated triacylglycerol, tripalmitin, in ER  likely to be relevant, since, at the relatively low palmitate/BSA ratios used here, the increase in triacylglycerol mass observed in MIN6 cells is due primarily to the incorporation of unsaturated fatty acid side-chains, as the increase in triacylglycerol levels was abolished by desaturase inhibitors . Increased formation of ceramide is a possibility since this is implicated in beta cell lipotoxicity [5, 6], and in other cell-types ceramide has been shown to perturb ER Ca2+ homeostasis .
Interestingly, islets of diabetic db/db mice also display abnormalities in ER Ca2+ mobilisation . Because protein folding is critically dependent on ER Ca2+ content, these previously demonstrated abnormalities might be linked to ER stress which, as we show here for the first time, is also a characteristic of the db/db model. In general, the induction of individual UPR genes was more pronounced in db/db islets than in palmitate-treated MIN6 cells. This is not surprising given the vastly different time frames involved, and because beta cells in the animal model would have been exposed to elevations in blood glucose as well as circulating fatty acids. Indeed, an enhancement of XBP1 splicing was recently demonstrated in islets exposed to high glucose for 24 h in vitro . This is consistent with our evidence of a prolonged activation of the IRE1/XBP1 arm both in db/db islets and fatty-acid-treated MIN6 cells. In contrast, ATF6 cleavage was transient in the cell system and was not observed at all in the animal model (results not shown), suggesting that this arm is either shorter lived than XBP1 signalling  or that the ATF6 cleavage product is rapidly degraded . On the other hand, DDIT3 induction was more modest in the islets of db/db mice than in the cellular model, but this is still likely to be of functional relevance over a period of months.
A key aspect of our work is the indication that ER stress actually occurs in beta cells derived from human subjects with type 2 diabetes. Because this depended on immunohistochemical analysis of archival pathology sections, it was not possible to investigate splicing of XBP1 mRNA, a definitive marker of ER stress. However, we provide evidence of induction of both HSPA5 and DNAJC3, which are predominately regulated in an ATF6- and XBP1-dependent manner, and hence are secondary to ER stress [28, 29]. Finally, we suggest that DDIT3 is also upregulated in human type 2 diabetes. This not only supports the presence of ER stress but also provides a potential mechanistic explanation for the apoptosis that appears to underlie the reductions in beta cell mass that contribute to type 2 diabetes in humans . As with BAX, however, increased production of DDIT3 is not always sufficient to trigger cell death, although upregulation is indicative of an enhanced apoptotic potential [47, 48]. Therefore, further work is needed to evaluate the contribution of DDIT3 to beta cell lipotoxicity, compared with other pro-apoptotic pathways triggered by ER stress, such as MAPK8 and caspase-12. Nevertheless, our studies provide strong evidence that ER stress may be a crucial factor contributing to beta cell apoptosis in the pathogenesis of type 2 diabetes. Accordingly, increasing the chaperone capacity of the ER represents a potential therapeutic approach for preventing the onset of the disease.
We thank E. Schmied, Z. Elgundi and R. P. Benito respectively for help with islet isolation, nucleofection and preparation of pancreas microarrays. We also thank A. Goodman for DNAJC3 antibodies, and C. Schmitz-Peiffer and G. Ramm for reviewing the manuscript. This work was supported by grants from the Diabetes Australia Research Trust and the National Health and Medical Research Council (NHMRC) of Australia (to D. R. Laybutt and T. J. Biden), a Cancer Institute NSW fellowship (to A. V. Biankin) and an NHMRC RD Wright Biomedical Career Development Award (to D. R. Laybutt).
Duality of interest
The authors declare that there is no duality of interest.
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