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Diabetologia

, Volume 45, Issue 11, pp 1515–1522 | Cite as

Abnormal metabolic fate of nitric oxide in Type I diabetes mellitus

  •  A. Milsom
  •  C. Jones
  •  J. Goodfellow
  •  M. Frenneaux
  •  J. Peters
  •  P. James
Article

Abstract

Aims/hypothesis. Reduced bioavailability of endothelium-derived nitric oxide is implicated in diabetic macrovascular and microvascular disease. In patients with diabetes, we hypothesised that protein glycosylation can alter nitric oxide binding affinity of haemoglobin and plasma proteins, hence reducing nitric oxide availability and causing an alteration in nitric oxide metabolism.

Methods. Binding of nitric oxide to haemoglobin was studied across a range of glycosylation levels in vitro (HbA1c 5.9 to 9.8%). In clinical studies nitrate, nitrite, nitrosyl haemoglobin and plasma nitrosothiols were measured in venous blood from 23 patients with uncomplicated Type I (insulin-dependent) diabetes mellitus and 17 non-diabetic control subjects. Samples were analysed at baseline and after nitric oxide was added ex vivo.

Results. Nitric oxide-haemoglobin binding was increased at a HbA1c greater than 8.5% compared with 5.9% (p<0.01). Basal nitrosyl haemoglobin was higher in diabetic patients compared with the control subjects (0.59±0.12 µmol/l vs 0.24±0.12 µmol/l, p<0.05). Plasma nitrosothiols, and nitrite and nitrate (NOx) concentrations were similar in diabetic patients compared with the control subjects (7.64±0.79 µmol/l vs 5.93±0.75 µmol/l, 13.98±2.44 µmol/l vs 12.44±2.15 µmol/l, respectively). In blood from diabetic patients, added nitric oxide was metabolised preferentially to nitrosyl haemoglobin and plasma nitrosothiols, with a twofold increase in nitrosyl haemoglobin observed across all concentrations of nitric oxide (p<0.05). These preferential increases correlated positively with HbA1c.

Conclusion/interpretation. Nitrosyl haemoglobin is increased in patients with Type I diabetes. Preferential metabolism to nitrosyl haemoglobin and nitrosothiols occurs after increases in nitric oxide. Our results show an accentuated association between nitric oxide and glycosylated proteins, especially deoxygenated haem. An altered metabolic fate of nitric oxide could influence microvascular regulation and tissue perfusion.

Haemoglobin glycosylation nitric oxide diabetes mellitus bioavailability metabolism nitrosyl haemoglobin 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  A. Milsom
    • 2
  •  C. Jones
    • 2
  •  J. Goodfellow
    • 2
  •  M. Frenneaux
    • 2
  •  J. Peters
    • 2
  •  P. James
    • 1
  1. 1.Wales Heart Research Institute, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
  2. 2.Wales Heart Research Institute, University of Wales College of Medicine, Heath Park, Cardiff, UK

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