Advertisement

Theoretical and Applied Genetics

, Volume 104, Issue 2–3, pp 177–183 | Cite as

A genome-specific repetitive DNA sequence from Oryza eichingeri : characterization, localization, and introgression to O. sativa

  • H. H. Yan
  • G. Q. Liu
  • Z. K. Cheng
  • X. B. Li
  • G. Z. Liu
  • S. K. Min
  • L.H. Zhu

Abstract 

In the course of transferring the brown planthopper resistance from a diploid, CC-genome wild rice species, Oryza eichingeri (IRGC acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. The O. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. Here we cloned a tandemly repetitive DNA sequence from O. eichingeri IRGC acc105163, and detected it in 25 introgression lines. This repetitive DNA sequence showed high specificity to the rice CC genome, but was absent from all the four tetraploid species with BBCC or CCDD genomes. The monomer in this repetitive DNA sequence is 325–366-bp long, with a copy number of about 5,000 per 1 C of the O. eichingeri genome, showing 88% homology to a repetitive DNA sequence isolated from Oryza officinalis (2n=2x=24, CC). Fluorescent in situ hybridization revealed 11 signals distributed over eight O. eichingeri chromosomes, mostly in terminal or subterminal regions.

Keywords Repetitive DNA sequence Oryza eichingeri Fluorescent in situ hybridization Introgression lines 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • H. H. Yan
    • 1
  • G. Q. Liu
    • 1
  • Z. K. Cheng
    • 1
  • X. B. Li
    • 1
  • G. Z. Liu
    • 1
  • S. K. Min
    • 2
  • L.H. Zhu
    • 1
  1. 1.Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, P.R. China e-mail: lhzhu@igtp.ac.cnCN
  2. 2.China National Rice Research Institute, Hangzhou 310006, P.R. ChinaCN

Personalised recommendations