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Theoretical and Applied Genetics

, Volume 104, Issue 2–3, pp 281–285 | Cite as

A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.)

  • N. Urasaki
  • M. Tokumoto
  • K. Tarora
  • Y. Ban
  • T. Kayano
  • H. Tanaka
  • H. Oku
  • I. Chinen
  • R. Terauchi

Abstract 

The random amplified polymorphic DNA (RAPD) technique was used to determine the sex of a dioecious species, Carica papaya L., with three sex types, male, female and hermaphrodite. A 450 bp marker fragment, named PSDM(Papaya Sex Determination Marker), exists in all male and hermaphrodite plants but not in the female plants so far analyzed. The DNA sequence of PSDM exhibited no significant similarity to previously reported sequences. A sequence-characterized amplified region (SCAR) marker, SCARps, was developed from PSDM to determine the sex of papaya. Southern hybridization, using PSDM as a probe, showed that PSDM exists in the male and hermaphrodite genomes, but not in the female genome. This result strongly suggests that PSDM is located on the chromosome region that is specific to the male and the hermaphrodite. SCARps is a suitable marker for the precise and rapid diagnosis of sex in papaya.

Keywords RAPD Sex determination Carica papaya Dioecious plant SCAR marker 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • N. Urasaki
    • 1
  • M. Tokumoto
    • 2
  • K. Tarora
    • 2
  • Y. Ban
    • 3
  • T. Kayano
    • 3
  • H. Tanaka
    • 3
  • H. Oku
    • 4
  • I. Chinen
    • 4
  • R. Terauchi
    • 1
  1. 1.Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, JapanJP
  2. 2.Okinawa Prefectural Agricultural Experiment Station, 4-222 Sakiyama, Naha, Okinawa 903-0814, Japan e-mail: urasaki@nousi.pref.okinawa.jp Tel.: +81-98-884-9900, Fax: +81-98-885-8000JP
  3. 3.National Institute of Agrobiological Resources, Kannondai, Tsukuba 305-8602, JapanJP
  4. 4.Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1, senbaru, Nishihara, Okinawa 903-0213, JapanJP

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