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Theoretical and Applied Genetics

, Volume 104, Issue 2–3, pp 273–280 | Cite as

Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using a two way pseudo-testcross strategy based on AFLP and SAMPL markers

  • A. Porceddu
  • E. Albertini
  • G. Barcaccia
  • E. Falistocco
  • M. Falcinelli
Original Article

Abstract 

The high versatility of the mode of reproduction and the retention of a pollen recognition system are the factors responsible for the extreme complexity of the genome in Poa pratensis L. Two genetic maps, one of an apomictic and one of a sexual genotype, were constructed using a two-way pseudo-testcross strategy and multiplex PCR-based molecular markers (AFLP and SAMPL). Due to the high ploidy level and the uncertainty of chromosome pairing-behavior at meiosis, only parent-specific single-dose markers (SDMs) that segregated 1:1 in an F1 mapping population (161 out of 299 SAMPLs, and 70 out of 275 AFLPs) were used for linkage analysis. A total of 41 paternal (33 SAMPLs and 8 AFLPs) and 47 maternal (33 SAMPLs and 14 AFLPs) SDMs, tested to be linked in coupling phase, were mapped to 7+7 linkage groups covering 367 and 338.4 cM, respectively. The comparison between the two marker systems revealed that SAMPL markers were statistically more efficient than AFLP ones in detecting parent-specific SDMs (75% vs 32.4%). There were no significant differences in the percentages of distorted marker alleles detected by the two marker systems (27.8% of SAMPLs vs 21.3% of AFLPs). The pairwise comparison of co-segregational groups for linkage detection between marker loci suggested that at least some of the P. pratensis chromosomes pair preferentially at meiosis-I.

Keywords AFLP Mapping Polyploids P. pratensis SAMPL 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • A. Porceddu
    • 3
  • E. Albertini
    • 1
  • G. Barcaccia
    • 2
  • E. Falistocco
    • 1
  • M. Falcinelli
    • 1
  1. 1.Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali. University of Perugia, Borgo XX Giugno 74, 06121, Perugia, ItalyIT
  2. 2.Dipartimento di Agronomia Ambientale e Produzioni Vegetali, University of Padova, Agripolis, Via Romea 16, 35020 Legnaro, Padova, ItalyIT
  3. 3.Current address: CNR-IMOF, Viale dell’ Universita` 133, 80055 Portici Napoli, ItalyIT

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