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PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.)

Theoretical and Applied Genetics volume 101, pages 364–371 (2000)Cite this article

Abstract 

The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.

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Authors and Affiliations

  1. Centre for Plant Conservation Genetics, Southern Cross University (SCU), Lismore, NSW, 2480, Australia+61-2 66222080, , , , , , AU

    S. Garland & R. Henry

  2. Yanco Agricultural Institute, NSW Agriculture, Yanco, NSW, 2703, Australia, , , , , , AU

    L. Lewin

  3. Grains Processing, BRI Australia Ltd, North Ryde, NSW, 2113, Australia, , , , , , AU

    A. Blakeney

Authors
  1. S. Garland
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  2. L. Lewin
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  3. A. Blakeney
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  4. R. Reinke
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  5. R. Henry
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Additional information

Received: 19 October 1999 / Accepted: 16 December 1999

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Garland, S., Lewin, L., Blakeney, A. et al. PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.). Theor Appl Genet 101, 364–371 (2000). https://doi.org/10.1007/s001220051492

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  • DOI: https://doi.org/10.1007/s001220051492

  • Keywords Fragrance
  • Rice
  • PCR marker
  • Oryza sativa