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Theoretical and Applied Genetics

, Volume 99, Issue 1–2, pp 300–304 | Cite as

Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization

  • D. Bardsley
  • A. Cuadrado
  • P. Jack
  • G. Harrison
  • A. Castilho
  • J. S. Heslop-Harrison

Abstract

 Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n=4x=28), which contains good resistance to eyespot, were analysed using fluorescent in situ hybridization. Probes used included rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns produced could be used to distinguish most chromosome arms and will aid in the identification of Ae. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation was identified, and other minor differences were found between the lines.

Key words Karyotype evolution Genome evolution In situ hybridization Plant breeding Alien genes Triticum ventricosum 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • D. Bardsley
    • 1
  • A. Cuadrado
    • 2
  • P. Jack
    • 3
  • G. Harrison
    • 1
  • A. Castilho
    • 1
  • J. S. Heslop-Harrison
    • 1
  1. 1.Department of Cell Biology, John Innes Centre, Colney, Norwich, NR4 7UH, UK Fax: +44-1603-456844 E-mail: pat.heslop-harrison@bbsrc.ac.ukGB
  2. 2.Department of Cellular Biology and Genetics, University of Alcaláde Henares, E-28871 Alcalá de Henares, Madrid, SpainES
  3. 3.Plant Breeding International, Maris Lane, Trumpington, Cambridge, CB2 2LQ, UKGB

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