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Theoretical and Applied Genetics

, Volume 96, Issue 6–7, pp 989–996 | Cite as

Identification and mapping of RAPD and RFLP markers linked to a fertility restorer gene for a new source of cytoplasmic male sterility in Beta vulgaris ssp. maritima

  • V. Laporte
  • D. Merdinoglu
  • P. Saumitou-Laprade
  • G. Butterlin
  • P. Vernet
  • J. Cuguen

Abstract

 The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility (CMS). This new source of CMS appears to be different from the mitotype E-associated CMS most frequently found in natural populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility (R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The comparison with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the ‘Owen’ CMS which is currently used in sugar beet breeding is reportedly linked to the monogermy locus, raising the question of a possible identity between the new CMS system and the ‘Owen’ CMS.

Key words Gynodioecy Cytoplasmic male sterility Restorer gene Bulk segregant analysis Beta vulgaris ssp. maritima 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • V. Laporte
    • 1
  • D. Merdinoglu
    • 2
  • P. Saumitou-Laprade
    • 1
  • G. Butterlin
    • 2
  • P. Vernet
    • 1
  • J. Cuguen
    • 1
  1. 1.Génétique et Evolution des Populations Végétales, URA CNRS 1185, Université de Lille I, Bâtiment SN2, F-59655 Villeneuve d’Ascq Cedex, France Fax: +33 320 4369 79 E-mail: joel.cuguenauniv-lille1.frFR
  2. 2.INRA, Station de Recherche sur les Grandes Cultures, 28 rue de Herrlisheim, 68021 Colmar Cedex, FranceFR

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