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Theoretical and Applied Genetics

, Volume 95, Issue 4, pp 660–663 | Cite as

Differentiation of phytoplasmas associated with sugarcane and gramineous weed white leaf disease and sugarcane grassy shoot disease by RFLP and sequencing

  • P. Wongkaew
  • Y. Hanboonsong
  • P. Sirithorn
  • C. Choosai
  • S. Boonkrong
  • T. Tinnangwattana
  • R. Kitchareonpanya
  • S. Damak

Abstract

 Restriction fragment length polymorphism (RFLP) and sequencing were used to elucidate the genetic relationship between phytoplasmas that cause white lead disease and grassy shoot disease in sugarcane and white leaf disease in gramineous weeds found in the cane-growing areas (Crowfoot grass, Bermuda grass and Brachiaria grass). A 1.35-kb DNA fragment encoding for the 16s rRNA was amplified by PCR using universal primers and analysed by digestion with nine restriction endonucleases. A DNA fragment containing the 3′ end of the 16s rRNA and the spacer region between the 16s rRNA and the tRNA(Ile) was amplified by PCR and sequenced. Analysis of the RFLP patterns and of the sequence showed that grassy shoot and white leaf diseases in sugarcane are caused by two different phytoplasmas. Sequence analysis of phytoplasma DNA obtained from three species of weeds showing symptoms of white leaf disease failed to detect any organism that is identical to those infecting the sugarcane. Moreover the phytoplasma species that infect the three types of gramineous weeds, although closely related, are nevertheless different

Key words Phytoplasma Sugarcane White leaf disease Grassy shoot disease Ribosomal RNA 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Wongkaew
    • 1
  • Y. Hanboonsong
    • 1
  • P. Sirithorn
    • 1
  • C. Choosai
    • 1
  • S. Boonkrong
    • 1
  • T. Tinnangwattana
    • 2
  • R. Kitchareonpanya
    • 2
  • S. Damak
    • 3
  1. 1.Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandTH
  2. 2.Northeastern Sugarcane Agricultural Centre, Office of the Cane and Sugar Board, Ministry of Industry, ThailandTH
  3. 3.Centre for Molecular Biology, Lincoln University, Canterbury, New zealandXX

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