Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc.

Abstract

 Somatic Hybrids between Sinapis alba and rapid-cycling Brassica oleracea were generated for transferring of resistance to Alternaria brassicae to B. oleracea. A. brassicae causes the significant disease black spot in cruciferous crops. A total of 27 plants were regenerated from protoplast fusion using 0, 5, 10, 20 and 30 krad γ-irradiation of the resistance donor and iodoacetate treatment of B. oleracea. All plants showed intermediate morphology with partially divided leaves and some trichomes on stems and leaves. Flow cytometry and banding patterns of the enzymes leucine amino peptidase (LAP) and phosphoglucose isomerase (PGI) confirmed the hybrid status of the regenerated plants. Some of the plants obtained from cuttings from the somatic hybrids showed a resistance to A. brassicae that was similar to that found in S. alba. The flowers of the somatic hybrids had reduced anthers with little pollen production.