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Theoretical and Applied Genetics

, Volume 100, Issue 2, pp 271–279 | Cite as

A spelt-specific γ-gliadin gene: discovery and detection

  • M. von Büren
  • J. Lüthy
  • P. Hübner
Original Article

Abstract 

Polymerase chain reaction (PCR) primers GAG5 and GAG6 were designed based on published γ-gliadin gene sequences and applied to 35 cultivars of closely related spelt (Triticum spelta L.) and hexaploid wheat (T. aestivum L.). Eight tetraploid durum wheat (T. durum Desf.) cultivars were included in the analysis. The obtained PCR products originated from two γ-gliadin genes which were mapped to homeologous chromosomes 1B and 1D and termed GAG56B and GAG56D, respectively. Two alleles of GAG56D differing in a 9-bp deletion/duplication and single nucleotide polymorphism were found. The 18 spelts tested and wheat cultivar ’Chinese Spring’ were discovered to carry a previously unknown γ-gliadin gene, while 16 wheat cultivars possessed its longer, already published allele. Two PCR-based detection systems for the diagnostic alleles were developed and applied. The occurrence of two alleles of GAG56B among the investigated durum wheats correlated with their expression of gluten quality markers γ-gliadins 42 or 45.

Key words Spelt Wheat γ-Gliadins PCR Molecular markers 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. von Büren
    • 1
  • J. Lüthy
    • 1
  • P. Hübner
    • 1
  1. 1.Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland e-mail: michael.vonbueren@ibc.unibe.ch, Fax: +41 (0) 31 631 4887CH

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