Abstract
Key message
Fine mapping of the maize QTL qSRC3, responsible for red silk, uncovered the candidate gene ZmMYB20, which encodes an R2R3-MYB transcription factor, has light-sensitive expression, and putatively regulates genes expression associated with anthocyanin biosynthesis.
Abstract
Colorless silk is a key characteristic contributing to the visual quality of fresh corn intended for market distribution. Nonetheless, the identification of Mendelian trait loci and associated genes that control silk color has been scarce. In this study, a F2 population arising from the hybridization of the single-segment substitution line qSRC3MT1 with red silk, carrying an introgressed allele from teosinte (Zea mays ssp. mexicana), and the recurrent maize inbred line Mo17, characterized by light green silk, was utilized for fine mapping. We found that the red silk trait is controlled by a semi-dominant genetic locus known as qSRC3, and its expression is susceptible to light-mediated inhibition. Moreover, qSRC3 explained 68.78% of the phenotypic variance and was delimited to a 133.2 kb region, which includes three genes. Subsequent expression analyses revealed that ZmMYB20 (Zm00001d039700), which encodes an R2R3-MYB transcription factor, was the key candidate gene within qSRC3. Yeast one-hybrid and dual-luciferase reporter assays provided evidence that ZmMYB20 suppresses the expression of two crucial anthocyanin biosynthesis genes, namely ZmF3H and ZmUFGT, by directly binding to their respective promoter regions. Our findings underscore the significance of light-inhibited ZmMYB20 in orchestrating the spatial and temporal regulation of anthocyanin biosynthesis. These results advance the production of colorless silk in fresh corn, responding to the misconception that fresh corn with withered colored silk is not fresh and providing valuable genetic resources for the improvement of sweet and waxy maize.
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References
Abdullah-Zawawi M, Ahmad-Nizammuddin N, Govender N, Harun S, Mohd-Assaad N, Mohamed-Hussein Z (2021) Comparative genome-wide analysis of WRKY, MADS-box and MYB transcription factor families in Arabidopsis and rice. Sci Rep 11(1):19678. https://doi.org/10.1038/s41598-021-99206-y
Adato A, Mandel T, Mintz-Oron S, Venger I, Levy D, Yativ M, Domínguez E, Wang Z, De V, Jetter R (2009) Fruit-surface flavonoid accumulation in tomato is controlled by a SlMYB12-regulated transcriptional network. PLoS Genet 5(12):e1000777. https://doi.org/10.1371/journal.pgen.1000777
Aharoni A, De Vos CH, Wein M, Sun Z, Greco R, Kroon A, Mol JN, O’Connell AP (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28(3):319–332. https://doi.org/10.1046/j.1365-313X.2001.01154.x
Albert N, Lewis D, Zhang H, Schwinn K, Davies K (2011) Members of an R2R3-MYB transcription factor family in Petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning. Plant J 65(5):771–784. https://doi.org/10.1111/j.1365-313X.2010.04465.x
An X, Tian Y, Chen K, Liu X, Liu D, Xie X, Cheng C, Cong P, Hao Y (2015) MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples. Plant Cell Physiol 56(4):650–662. https://doi.org/10.1093/pcp/pcu205
Anwar M, Wang G, Wu J, Waheed S, Allan AC, Zeng L (2018) Ectopic overexpression of a novel R2R3-MYB, NtMYB2 from Chinese narcissus represses anthocyanin biosynthesis in tobacco. Molecules 23(4):781. https://doi.org/10.3390/molecules23040781
Aoki H, Kuze N, Kato Y, Gen SE, Inc F (2001) Anthocyanin isolated from purple corn (Zea mays L). Foods Food Ingred J Jpn 199
Azuma A, Kobayashi S, Mitani N, Shiraishi M, Yamada M, Ueno T, Kono A, Yakushiji H, Koshita Y (2008) Genomic and genetic analysis of Myb-related genes that regulate anthocyanin biosynthesis in grape berry skin. Theor Appl Genet 117(6):1009–1019. https://doi.org/10.1007/s00122-008-0840-1
Bai S, Tao R, Tang Y, Yin L, Ma Y, Ni J, Yan X, Yang Q, Wu Z, Zeng Y, Teng Y (2019) BBX16, a B-box protein, positively regulates light-induced anthocyanin accumulation by activating MYB10 in red pear. Plant Biotechnol J 17(10):1985–1997. https://doi.org/10.1111/pbi.13114
Bennett C, Funsueb S, Kittiwachana S, Sookwong P, Mahatheeranont S (2023) Mineral elements and their relation to anthocyanin content in pigmented rice plants using definitive screening design and self-organizing maps. J Sci Food Agric 103(9):4535–4544. https://doi.org/10.1002/jsfa.12534
Biswas PL, Nath UK, Ghosal S, Goswami G, Uddin MS, Ali OM, Latef AAHA, Laing AM, Gao YM, Hossain A (2021) Introgression of bacterial blight resistance genes in the rice cultivar ciherang: response against Xanthomonas oryzae pv. oryzae in the F6 generation. Plants 10(10):2048. https://doi.org/10.3390/plants10102048
Chaikam V, Nair S, Babu R, Martinez L, Tejomurtula J, Boddupalli P (2015) Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression. Theor Appl Genet 128:159–171. https://doi.org/10.1007/s00122-014-2419-3
Chen L, Hu B, Qin Y, Hu G, Zhao J (2019) Advance of the negative regulation of anthocyanin biosynthesis by MYB transcription factors. Plant Physiol Biochem 136:178–187. https://doi.org/10.1016/j.plaphy.2018.08.016
Cheynier V, Comte G, Davies K, Lattanzio V, Martens S (2013) Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiol Biochem 72:1–20. https://doi.org/10.1016/j.plaphy.2013.05.009
Chotechuang N, Di Gianvincenzo P, Chen CG, Nardi AN, Padró D, Boonla C, Ortore MG, D’Abramo M, Moya SE (2023) A study of cyanidin/alginate complexation: influence of pH in assembly and chiral properties. Carbohydr Polym 315:120957. https://doi.org/10.1016/j.carbpol.2023.120957
Cobb JN, Biswas PS, Platten JD (2018) Back to the future: revisiting MAS as a tool for modern plant breeding. Theor Appl Genet 132(3):647–667. https://doi.org/10.1007/s00122-018-3266-4
Colquhoun TA, Kim JY, Wedde AE, Levin LA, Schmitt KC, Schuurink RC, Clark DG (2011) PhMYB4 fine-tunes the floral volatile signature of Petunia×hybrida through PhC4H. J Exp Bot 62(3):1133–1143. https://doi.org/10.1093/jxb/erq342
Cone KC, Burr FA, Burr B (1986) Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci USA 83(24):9631–9635. https://doi.org/10.1073/pnas.83.24.9631
Dai Z, Zhang Y, Wang X, Yang Q, Wang Y, Li B (2021) Fine mapping and candidate gene analysis of silk color gene qSC10 in maize(Zea mays L.). J Plant Genet Resour 22(5):1383–1393. https://doi.org/10.13430/j.cnki.jpgr.20210217001
Fu Z, Wang L, Shang H, Dong X, Jiang H, Zhang J, Wang H, Li Y, Yuan X, Meng S (2019) An R3-MYB gene of phalaenopsis, MYBx1, represses anthocyanin accumulation. Plant Growth Regul 88:129–138. https://doi.org/10.1007/s10725-019-00493-3
Gavazzi G, Mereghetti M, Consonni G, Tonelli C (1990) Sn, a light-dependent and tissue-specific gene of maize: the genetic basis of its instability. Genetics 125(1):193–199. https://doi.org/10.1016/0735-0651(90)90042-E
Geng P, Zhang S, Liu J, Zhao C, Wu J, Cao Y, Fu C, Han X, He H, Zhao Q (2020) MYB20, MYB42, MYB43, and MYB85 regulate phenylalanine and lignin biosynthesis during secondary cell wall formation. Plant Physiol 182(3):1272–1283. https://doi.org/10.1104/pp.19.01070
Gordeeva E, Shoeva O, Mursalimov S, Adonina I, Khlestkina E (2022) Fine points of marker-assisted pyramiding of anthocyanin biosynthesis regulatory genes for the creation of black-grained bread wheat (Triticum aestivum L.) lines. Agronomy 12(12):2934. https://doi.org/10.3390/agronomy12122934
Guo X, Wang Y, Zhai Z, Huang T, Zhao D, Peng X, Feng C, Xiao Y, Li T (2018) Transcriptomic analysis of light-dependent anthocyanin accumulation in bicolored cherry fruits. Plant Physiol Biochem 130:663–677. https://doi.org/10.1016/j.plaphy.2018.08.016
Hu Y, Cheng H, Zhang Y, Zhang J, Niu S, Wang X, Li W, Zhang J, Yao Y (2021) The MdMYB16/MdMYB1-miR7125-MdCCR module regulates the homeostasis between anthocyanin and lignin biosynthesis during light induction in apple. New Phytol 231(3):1105–1122. https://doi.org/10.1111/nph.17431
Ibitoye DO, Akin-Idowu PE (2013) Marker-assisted-selection (MAS): a fast track to increase genetic gain in horticultural crop breeding. Afr J Biotech 9(52):8889–8895. https://doi.org/10.1186/1748-7188-6-22
Jung C, Griffiths H, De J, Cheng S, Bodis M, Kim T, De J (2009) The potato developer (D) locus encodes an R2R3 MYB transcription factor that regulates expression of multiple anthocyanin structural genes in tuber skin. Theor Appl Genet 120(1):45–57. https://doi.org/10.1007/s00122-009-1158-3
Kim D, Jeon S, Yanders S, Park S, Kim H, Kim S (2022) MYB3 plays an important role in lignin and anthocyanin biosynthesis under salt stress condition in Arabidopsis. Plant Cell Rep 41(7):1549–1560. https://doi.org/10.1007/s00299-022-02878-7
Kobayashi S, Goto-Yamamoto N, Hirochika H (2005) Association of VvmybA1 gene expression with anthocyanin production in grape (Vitis vinifera) skin-color mutants. J Jpn Soc Hortic Sci 74(3):196–203. https://doi.org/10.2503/jjshs.74.196
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874. https://doi.org/10.1093/molbev/msw054
Lander E, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1(2):174–181. https://doi.org/10.1016/j.ygeno.2008.12.003
Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25(14):1754–1760. https://doi.org/10.1093/bioinformatics/btp324
Li C, Ng C, Fan L (2015) MYB transcription factors, active players in abiotic stress signaling. Environ Exp Bot 114:80–91. https://doi.org/10.1016/j.envexpbot.2014.06.014
Li J, Ren L, Gao Z, Jiang M, Liu Y, Zhou L, He Y, Chen H (2017) Combined transcriptomic and proteomic analysis constructs a new model for light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L.). Plant Cell Environ 40(12):3069–3087. https://doi.org/10.1111/pce.13074
Li L, Li S, Ge H, Shi S, Li D, Liu Y, Chen H (2021) A light-responsive transcription factor SmMYB35 enhances anthocyanin biosynthesis in eggplant (Solanum melongena L.). Planta 255(1):12. https://doi.org/10.1007/s00425-021-03698-x
Liu J, Qu J, Yang C, Tang D, Li J, Lan H, Rong T (2015) Development of genome-wide insertion and deletion markers for maize, based on next-generation sequencing data. BMC Genomics 16(1):601. https://doi.org/10.1186/s12864-015-1797-5
Liu C, Qi X, Song L, Chen L, Dong Y, Pan F, Zheng W, Li Y, Zhao B, Guo W, Li M, Wang Z (2023) Large-fragment deletion encompasses the R2R3 MYB transcription factor, PavMYB10.1, causes yellow fruits in sweet cherry (Prunus avium L.). Sci Hortic 309:111648. https://doi.org/10.1016/j.scienta.2022.111648
Livak K, Schmittgen T (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262
Lloyd A, Brockman A, Aguirre L, Campbell A, Bean A, Cantero A, Gonzalez A (2017) Advances in the MYB-bHLH-WD repeat (MBW) pigment regulatory model: addition of a WRKY factor and co-option of an anthocyanin MYB for betalain regulation. Plant Cell Physiol 58(9):1431–1441. https://doi.org/10.1093/pcp/pcx075
Ma Z, Li W, Mao J, Li W, Chen B (2019) Synthesis of light-inducible and light-independent anthocyanins regulated by specific genes in grape “Marselan” (V. Vinifera L.). PeerJ 7(1):e6521. https://doi.org/10.7717/peerj.6521
Ma A, Wang D, Lu H, Wang H, Qin Y, Hu G, Zhao J (2021a) LcCOP1 and LcHY5 control the suppression and induction of anthocyanin accumulation in bagging and debagging litchi fruit pericarp. Sci Hortic 287:110281. https://doi.org/10.1016/j.scienta.2021.110281
Ma Y, Ma X, Gao X, Wu W, Zhou B (2021b) Light induced regulation pathway of anthocyanin biosynthesis in plants. Int J Mol Sci 22(20):11116. https://doi.org/10.3390/ijms222011116
Marc H, Roman U (2012) UV-B photoreceptor-mediated signalling in plants. Trends Plant Sci 17(4):230–237. https://doi.org/10.1016/j.tplants.2012.01.007
Matsui K, Umemura Y, Ohme-Takagi M (2008) AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. Plant J 55(6):954–967. https://doi.org/10.1111/j.1365-313X.2008.03565.x
McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M, DePristo MA (2010) The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res 20(9):1297–1303. https://doi.org/10.1101/gr.107524.110
Meng X, Yang D, Li X, Zhao S, Sui N, Meng Q (2015) Physiological changes in fruit ripening caused by overexpression of tomato SlAN2, an R2R3-MYB factor. Plant Physiol Biochem 89:24–30. https://doi.org/10.1016/j.plaphy.2015.02.005
Meyer C, Paparozzi ET, Josiah SJ, Blankenship EM (2004) Assessing color change in woody floral stems. HortScience 39(4):836A – 836. https://doi.org/10.21273/HORTSCI.39.4.836A
Ohno S, Ueno M, Doi M (2020) Differences in the CaMYBA genome between anthocyanin-pigmented cultivars and non-pigmented cultivars in pepper (Capsicum annuum). Hortic J 89(1):30–36. https://doi.org/10.2503/hortj.UTD-097
Pérez-Díaz J, Pérez-Díaz J, Madrid-Espinoza J, González-Villanueva E, Moreno Y, Ruiz-Lara S (2016) New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in the flowers of transgenic tobacco. Plant Mol Biol 90(1–2):63–76. https://doi.org/10.1007/s11103-015-0394-y
Pooma W, Gersos C, Grotewold E (2002) Transposon insertions in the promoter of the Zea mays a1 gene differentially affect transcription by the Myb factors P and C1. Genetics 161(2):793–801. https://doi.org/10.1093/genetics/161.2.793
Premathilake AT, Ni J, Bai S, Tao R, Teng Y (2020) R2R3-MYB transcription factor PpMYB17 positively regulates flavonoid biosynthesis in pear fruit. Planta 252(4):59. https://doi.org/10.1007/s00425-020-03473-4
Qin H, Yan M, Wang Z, Guo Y, Cai Y (2012) QTL mapping for anthocyanin and melanin contents in maize kernel. Acta Agron Sin 38(2):275–284. https://doi.org/10.3724/SP.J.1006.2012.00275
Ram M, Prasad K, Kaur C, Singh S, Kuma A (2011) Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels. Plant Cell Tissue Organ Cult 104(2):171–179. https://doi.org/10.1007/s11240-010-9814-5
Rao G, Reddy J, Variar M, Mahender A (2016) Molecular breeding to improve plant resistance to abiotic stresses. Adv Plant Breed Strateg 2:283–326. https://doi.org/10.1007/978-3-319-22518-0_8
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard R (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81(24):8014–8018. https://doi.org/10.1073/pnas.81.24.8014
Sharma M, Chai C, Morohashi K, Grotewold E, Snook M (2012) Expression of flavonoid 3’-hydroxylase is controlled by P1, the regulator of 3-deoxyflavonoid biosynthesis in maize. BMC Plant Biol 12(1):196. https://doi.org/10.1186/1471-2229-12-196
Shimizu Y, Maeda K, Shimomura K (2010) Methyl jasmonate induces anthocyanin accumulation in Gynura bicolor cultured roots. Plant Physiol Biochem 46(5):460–465. https://doi.org/10.1007/s11627-010-9294-7
Song S, Qi T, Fan M, Zhang X, Gao H, Huang H, Wu D, Guo H, Xie D (2013) The bHLH subgroup IIId factors negatively regulate jasmonate-mediated plant defense and development. PLoS Genet 9(7):e1003653. https://doi.org/10.1371/journal.pgen.1003653
Stevens T, Degner R, Morgan K, Debodisco C, House A (2003) Market development strategies for florida fresh sweet corn: findings from a consumer survey, 2001. Food Resour Econ. https://doi.org/10.32473/edis-fe377-2003
Sun L, Lin X, Xie H, Fu Z, Tang J (2007) The tagging molecular markers for red silk gene in maize inbred Line K12. J Henan Agric Univ 41(5):480–482. https://doi.org/10.3969/j.issn.1000-2340.2007.05.002
Sun C, Deng L, Du M, Zhao J, Chen Q, Huang T, Jiang H, Li C, Li C (2020) A transcriptional network promotes anthocyanin biosynthesis in tomato flesh. Mol Plant 13(1):17. https://doi.org/10.1016/j.molp.2019.10.010
Sun X, Zhang Q, Zhou H (2021) Anthocyanins: from biosynthesis regulation to crop improvement. Bot Lett 168(4):546–557. https://doi.org/10.1080/23818107.2021.1909498
Takos AM, Jaffé FW, Jacob SR, Bogs J, Robinson SP, Walker AR (2006) Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol 143(3):1216–1232. https://doi.org/10.1104/pp.106.088104
Tuberosa R, Salvi S (2006) QTLs and genes for tolerance to abiotic stress in cereals. Cereal Genomics. https://doi.org/10.1007/1-4020-2359-6-9
Wang P, Lu Y, Zheng M, Rong T, Tang Q (2011) RAPD and internal transcribed spacer sequence analyses reveal Zea nicaraguensis as a section Luxuriantes species close to Zea luxurians. PLoS ONE 6(4):e16728. https://doi.org/10.1371/journal.pone.0016728
Wang Y, Sun J, Wang N, Xu H, Chen X (2018) MdMYBL2 helps regulate cytokinin-induced anthocyanin biosynthesis in red-fleshed apple (Malus sieversii f. niedzwetzkyana) callus. Funct Plant Biol 46(2):187–196. https://doi.org/10.1071/FP17216
Wang X, Niu Y, Zheng Y (2021) Multiple functions of MYB transcription factors in abiotic stress responses. Int J Mol Sci 22(11):6125. https://doi.org/10.3390/ijms22116125
Xu Z, Feng K, Que F, Wang F, Xiong A (2017) A MYB transcription factor, DcMYB6, is involved in regulating anthocyanin biosynthesis in purple carrot taproots. Sci Rep 7:45324. https://doi.org/10.1038/srep45324
Xu Z, Hua J, Wang F, Cheng Z, Weng J (2020) Marker-assisted selection of qMrdd8 to improve maize resistance to rough dwarf disease. Breed Sci 70(2):183–192. https://doi.org/10.1270/jsbbs.19110
Xu Z, Wang J, Ma Y, Wang F, Wang J, Zhang Y, Hu X (2023) The bZIP transcription factor SlAREB1 regulates anthocyanin biosynthesis in response to low temperature in tomato. Plant J 115(1):205–219. https://doi.org/10.1111/tpj.16224
Yamagishi M, Shimoyamada Y, Nakatsuka T, Masuda K (2010) Two R2R3-MYB genes, homologs of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of asiatic hybrid lily. Plant Cell Physiol 51(3):463–474. https://doi.org/10.1093/pcp/pcq011
Yan H, Pei X, Zhang H, Li X, Zhao X (2021) MYB-mediated regulation of anthocyanin biosynthesis. Int J Mol Sci 22(6):3103. https://doi.org/10.3390/ijms22063103
Yang Y, Wu C, Shan W, Wei W, Zhao Y, Kuang J, Chen J, Jiang Y, Lu W (2023) Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening. Hortic Res 10(1):220–231. https://doi.org/10.1093/hr/uhac243
Yin X, Zhang Y, Wang B, Zhao Y, Irfan M, Chen L, Feng Y (2021) Regulation of MYB transcription factors of anthocyanin synthesis in lily flowers. Front Plant Sci 12:761668. https://doi.org/10.3389/fpls.2021.761668
Zoratti L, Karppinen K, Luengo E, Häggman H, Jaakola L (2014) Light-controlled flavonoid biosynthesis in fruits. Front Plant Sci 5:534. https://doi.org/10.3389/fpls.2014.00534
Acknowledgements
We are grateful for the financial support for this work from the Sichuan Science and Technology Program (2022NSFSC0151), National Basic Research Program of China (the “973” project, 2014CB138203), the National Natural Science Foundation of China (31101161), and the Double-Support Plan of Sichuan Agricultural University. We would like to thank MogoEdit (https://www.mogoedit.com) for its English editing during the preparation of this manuscript.
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This research was supported by The Sichuan Science and Technology Program (2022NSFSC0151), the National Basic Research Program of China (the “973” project, 2014CB138203), the National Natural Science Foundation of China (31101161), and the Double-Support Plan of Sichuan Agricultural University.
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JL, TR, and XW conceived and designed the research. XW, YZ, and CY wrote and revised the manuscript. JY, RB, ZL, PM, SY, and XW conducted the experiments, with the assistance of JN, PL, LW, GZ, WD, DT, and HW analyzed the data. All authors read and approved the final manuscript.
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Wang, X., Zhou, Y., You, C. et al. Fine mapping and candidate gene analysis of qSRC3 controlling the silk color in maize (Zea mays L.). Theor Appl Genet 137, 90 (2024). https://doi.org/10.1007/s00122-024-04598-w
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DOI: https://doi.org/10.1007/s00122-024-04598-w