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Identification and molecular mapping of a major gene conferring resistance to Phytophthora sansomeana in soybean ‘Colfax’

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Abstract

Key message

The first single dominant resistance gene contributing major resistance to the oomycete pathogen Phytophthora sansomeana was identified and mapped from soybean ‘Colfax’.

Abstract

Phytophthora root rot (PRR) is one of the most important diseases in soybean (Glycine max). PRR is well known to be caused by Phytophthora sojae, but recent studies showed that P. sansomeana also causes extensive root rot of soybean. Depending upon the isolate, it might produce aggressive symptoms, especially in seeds and seedlings. Unlike P. sojae which can be effectively managed by Rps genes, no known major resistance genes have yet been reported for P. sansomeana. Our previous study screened 470 soybean germplasm lines for resistance to P. sansomeana and found that soybean ‘Colfax’ (PI 573008) carries major resistance to the pathogen. In this study, we crossed ‘Colfax’ with a susceptible parent, ‘Senaki’, and developed three mapping populations with a total of 234 F2:3 families. Inheritance pattern analysis indicated a 1:2:1 ratio for resistant: segregating: susceptible lines among all the three populations, indicating a single dominant gene conferring the resistance in ‘Colfax’ (designated as Rpsan1). Linkage analysis using extreme phenotypes anchored Rpsan1 to a 30 Mb region on chromosome 3. By selecting nine polymorphic SNP markers within the region, Rpsan1 was genetically delimited into a 21.3 cM region between Gm03_4487138_A_C and Gm03_5451606_A_C, which corresponds to a 1.06 Mb genomic region containing nine NBS-LRR genes based on Gmax2.0 assembly. The mapping results were then validated using two breeding populations derived from ‘E12076T-03’ × ‘Colfax’ and ‘E16099’ × ‘Colfax’. Marker-assisted resistance spectrum analyses with 9 additional isolates of P. sansomeana indicated that Rpsan1 may be effective towards a broader range of P. sansomeana isolates and has strong merit in protecting soybean to this pathogen in the future.

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Data availability

The phenotype and genotype data generated in this study is available in ‘Supplementary Data’. Additional information is available upon request.

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Acknowledgements

We thank the funding support from Michigan Soybean Committee, USDA National Institute of Food and Agriculture (Hatch project 1011788), AgBioResearch at Michigan State University (Project No. MICL02013), and United Soybean Board (24-209-S-A-1-A and 2331-202-0201). We also thank the Overseas Training Program for Young-Backbone Teachers of Hebei Agricultural University to WL. We are also thankful to the State Scholarship Fund from China Scholarship Council (201908230370) for Zhanguo Zhang.

Funding

United Soybean Board (24-209-S-A-1-A and 2331-202-0201). AgBioResearch at Michigan State University (Project No. MICL02824).

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Authors and Affiliations

  1. Department of Plant, Soil and Microbial Sciences, Michigan State University, 1066 Bogue St., East Lansing, MI, 48824-1325, USA

    Feng Lin, Muhammad Salman, Zhanguo Zhang, Austin G. McCoy, Raju Thada Magar, Drew Mitchell, Cuihua Gu, Martin I. Chilvers & Dechun Wang

  2. Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Punjab, 38000, Pakistan

    Muhammad Salman

  3. Northeast Agricultural University, National Soybean Engineering Research Center, Harbin, 150030, Heilongjiang Province, China

    Zhanguo Zhang

  4. North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University, Lekai South Street 2596, Baoding, 071001, Hebei Province, China

    Wenlong Li

  5. Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611, USA

    Meixia Zhao

Authors
  1. Feng Lin
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  2. Muhammad Salman
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  3. Zhanguo Zhang
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  4. Austin G. McCoy
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  5. Wenlong Li
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  8. Meixia Zhao
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  10. Martin I. Chilvers
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Contributions

DW and FL designed the research. FL, MS, ZZ, AGM, WL, RTM, DM, MX, and CG carried out the experiments. FL and MS analyzed the data and developed the draft manuscript. AGM, MIC, and DW edited the manuscript. All authors revised the manuscript and contributed to the final manuscript.

Corresponding authors

Correspondence to Feng Lin or Dechun Wang.

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Communicated by Volker Hahn.

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Lin, F., Salman, M., Zhang, Z. et al. Identification and molecular mapping of a major gene conferring resistance to Phytophthora sansomeana in soybean ‘Colfax’. Theor Appl Genet 137, 55 (2024). https://doi.org/10.1007/s00122-024-04556-6

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