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Fine mapping and analysis of candidate genes for qBT2 and qBT7.2 locus controlling bolting time in radish (Raphanus sativus L.)

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Two major QTLs for bolting time in radish were mapped to chromosome 02 and 07 in a 0.37 Mb and 0. 52 Mb interval, RsFLC1 and RsFLC2 is the critical genes.

Abstract

Radish (Raphanus sativus L.) is an important vegetable crop of Cruciferae. The premature bolting and flowering reduces the yield and quality of the fleshy root of radish. However, the molecular mechanism underlying bolting and flowering in radish remains unknown. In YZH (early bolting) × XHT (late bolting) F2 population, a high-density genetic linkage map was constructed with genetic distance of 2497.74 cM and an average interval of 2.31 cM. A total of nine QTLs for bolting time and two QTLs for flowering time were detected. Three QTLs associated with bolting time in radish were identified by QTL-seq using radish GDE (early bolting) × GDL (late bolting) F2 population. Fine mapping narrowed down qBT2 and qBT7.2 to an 0.37 Mb and 0.52 Mb region on chromosome 02 and 07, respectively. RNA-seq and qRT-PCR analysis showed that RsFLC1 and RsFLC2 were the candidate gene for qBT7.2 and qBT2 locus, respectively. Subcellular localization exhibited that RsFLC1 and RsFLC2 were mainly expressed in the nucleus. A 1856-bp insertion in the first intron of RsFLC1 was responsible for bolting time. Overexpression of RsFLC2 in Arabidopsis was significantly delayed flowering. These findings will provide new insights into the exploring the molecular mechanism of late bolting and promote the marker-assisted selection for breeding late-bolting varieties in radish.

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Data availability

All data generated or analyzed during the course of this study are included in the article. The transcriptome sequencing reads have been deposited into the NCBI sequence read archive (SRA) under accession no. PRJNA1020617. All data and research materials or this study are available upon reasonable request from the corresponding author.

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Acknowledgements

We would like to thank Ruhong Xu from the School of Agriculture of Guizhou University support for providing the phenotypic and genotypic data analysis.

Funding

This study was funded by the National Natural Science Foundation of China (31960598), Guizhou Provincial Department of Agriculture and Rural Affairs Project (Qiankehe Platform Talent-CXTD [2022]003), Key core technology research project for mountainous agriculture in Guizhou Province (GZNYGJHX-2023013), Platform construction project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province (Qian Jiao Ji [2022] No. 040).

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  1. Yueyue Jin and Xiaobo Luo have contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture, Guizhou University, Guiyang, 550003, Guizhou, China

    Yueyue Jin, Yadong Li, Xiao Peng, Linjun Wu, Guangqian Yang, Xiuhong Xu, Yun Pei, Wei Li & Wanping Zhang

  2. Guizhou Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, 550003, Guizhou, China

    Xiaobo Luo

  3. Institute of Vegetable Industry Technology Research, Guizhou University, Guiyang, 550003, Guizhou, China

    Yueyue Jin, Yadong Li, Xiao Peng, Linjun Wu, Guangqian Yang, Xiuhong Xu, Yun Pei & Wanping Zhang

  4. Guizhou Higher Education Facility Vegetable Engineering Reseach Centre, Guizhou University, Guiyang, 550003, Guizhou, China

    Wei Li

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  1. Yueyue Jin
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Contributions

YYJ and XBL have prepared a draft manuscript; YYJ conducted experiments; YDL, XP, GQY and LJW participated in the field experiment. XBL and WPZ designed experiments; XHX, YP, WL assisted in the experiment.

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Correspondence to Wanping Zhang.

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Jin, Y., Luo, X., Li, Y. et al. Fine mapping and analysis of candidate genes for qBT2 and qBT7.2 locus controlling bolting time in radish (Raphanus sativus L.). Theor Appl Genet 137, 4 (2024). https://doi.org/10.1007/s00122-023-04503-x

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