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Mapping of Rf20(t), a minor fertility restorer gene for rice wild abortive cytoplasmic male sterility in the maintainer line ‘Zhenshan97B’

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Abstract

Three-line hybrid rice has primarily been developed on wild abortive (WA)-type cytoplasmic male sterility (CMS) and has helped increase the yield of rice globally. The development of WA-type CMS lines and hybrids was expedited through the identification and mapping of the fertility restorer gene (Rf) in maintainers. This study observed fertile plants in WA-TianfengA/Zhenshan97B//TianfengB population, indicating that the maintainer line ‘Zhenshan97B’ should carry Rfs for WA-type CMS. Several advanced backcross populations were generated with the genetic background of the ‘WA-TianfengA,’ and the pollen fertility levels of the backcrossed individuals in BC3F1, BC4F1 and BC4F2 populations are governed by a new gene, Rf20(t), from ‘Zhenshan97B.’ Employing bulk segregant analysis of fertile and sterile pools from the BC4F1 population, Rf20(t) was genetically mapped to a candidate region on chromosome 10. Subsequently, Rf20(t) was located between RM24883 and RM24919 through recombination analysis of molecular markers using the BC4F2 population. Implementing a substitution mapping strategy, Rf20(t) was ultimately mapped to a 245-kb region between the molecular markers STS10-122 and STS10-126 and obtained the most likely candidate gene LOC_Os10g02650, which is predicted to encode pentatricopeptide repeat-containing (PPR) protein. These results enhance our understanding of the fertility restoration of WA-type CMS lines, facilitating the development of high-quality pairs of WA-type CMS and maintainer lines.

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All data generated or used during the study appear in the submitted article.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation [Grant Number 32072031]; the Zhongshan Biological Breeding Laboratory [BM2022008-03]; the Promoting Project for Open Competition Mechanism to Select the Best Candidates of Jiangsu Seed Industry [grant numbers JBGS[2021] 001, JBGS[2021] 040]; the Key University Science Research Project of Jiangsu Province [Grant Number 22KJA210004]; the Modern Agriculture Foundation of Yangzhou [Grant Number SNY2022000047]; and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Funding

This study was funded by The National Natural Science Foundation [grant number 32072031]; The Zhongshan Biological Breeding Laboratory Foundation [BM2022008-03]; The Promoting Project for Open Competition Mechanism to Select the Best Candidates of Jiangsu Seed Industry [grant numbers JBGS[2021] 001, JBGS[2021] 040]; The Key University Science Research Project of Jiangsu Province [grant number 22KJA210004]; The Modern Agriculture Foundation of Yangzhou [grant number SNY2022000047]; and The Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Yang Liu and Zuopeng Xu have contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou, 225009, China

    Yang Liu, Zuopeng Xu, Yuanyue Du, Benxi Huang, Meng Li, Lijia Zhang, Qiaoquan Liu, Shuzhu Tang & Honggen Zhang

  2. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Qiaoquan Liu, Shuzhu Tang & Honggen Zhang

  3. School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China

    Xiangqiang Zhao

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  1. Yang Liu
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Contributions

HZ and ZX conducted the data analysis and drafted the manuscript. YL and ZX performed the phenotypic evaluation and data analysis. YD performed qRT-PCR and genome sequence. BX, ML LZ and XZ participated in the construction of the testcross populations. QL and ST participated in design of the study. HZ designed the study and revised the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Honggen Zhang.

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Liu, Y., Xu, Z., Du, Y. et al. Mapping of Rf20(t), a minor fertility restorer gene for rice wild abortive cytoplasmic male sterility in the maintainer line ‘Zhenshan97B’. Theor Appl Genet 136, 248 (2023). https://doi.org/10.1007/s00122-023-04486-9

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