Abstract
Key message
Yield and quality tests of wheat lines derived from RWG35 show they carry little, or no linkage drag and are the preferred source of Sr47 for stem rust resistance.
Abstract
Three durum wheat (Triticum turgidum L. subsp. durum) lines, RWG35, RWG36, and RWG37 carrying slightly different Aegilops speltoides introgressions, but each carrying the Sr47 stem rust resistance gene, were backcrossed to three durum and three hard red spring (HRS) wheat (Triticum aestivum L.) cultivars to produce 18 backcross populations. Each population was backcrossed to the recurrent parent six times and prepared for yield trials to test for linkage drag. Lines carrying the introgression (S-lines) were compared to euploid sibling lines (W-lines) and their parent. Yield trials were conducted from 2018 to 2021 at three locations. Three agronomic and several quality traits were studied. In durum, lines derived from RWG35 had little or no linkage drag. Lines derived from RWG36 and RWG37 still retained linkage drag, most notably involving yield and thousand kernel weight, but also test weight, falling number, kernel hardness index, semolina extract, semolina protein content, semolina brightness, and peak height. In HRS wheat, the results were more complex, though the general result of RWG35 lines having little or no linkage drag and RWG36 and RWG37 lines retaining linkage drag still applied. But there was heterogeneity in the Glenn35S lines, and Linkert lines had problems combining with the Ae. speltoides introgressions. We concluded that introgressions derived from RWG35 either had eliminated linkage drag or any negative effects were minor in nature. We recommend that breeders who wish to incorporate Sr47 into their cultivars should work exclusively with germplasm derived from RWG35.
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Acknowledgements
We would like to acknowledge Dr. James Anderson for providing seed of Linkert. We also acknowledge Sally Mann, Thor Selland, Andre Miranda, Jason Axtman, and the late Stan Stancyk, for their technical assistance in the field plots; and Christopher Cossette, Kaitlin Erdmann, Amy Stolt, and Mary Valenzuela for their technical assistance in the quality analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, ARS. USDA is an equal opportunity provider and employer.
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This work was supported in part by USDA-ARS CRIS Project No. 3060-21000-038-00D, 3060-43440-015-00D, and 2030-21430-014-00D and Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) project (Grant Number OPPGD1389) funded through a grant from the Bill & Melinda Gates Foundation and UK Department for International Development and administered by Cornell University, Ithaca, New York.
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SSX and DLK initiated and designed the project. DLK, SX, and JF developed introgression and breeding lines. DLK performed stem rust tests and marker analysis. DLK, ES, JU, EE, and AG conducted field trials. LD performed quality trait analysis. DLK and SSX prepared the manuscript, with revisions from all authors.
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Klindworth, D.L., Salsman, E., Underdahl, J. et al. Linkage drag analysis in three Aegilops speltoides introgressions carrying Sr47 in modern durum and hard red spring wheat germplasm. Theor Appl Genet 136, 168 (2023). https://doi.org/10.1007/s00122-023-04409-8
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DOI: https://doi.org/10.1007/s00122-023-04409-8