Skip to main content
Log in

Physical mapping of the wheat genes in low-recombination regions: radiation hybrid mapping of the C-locus

  • Original Article
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript


Key Message

This work reports the physical mapping of an important gene affecting spike compactness located in a low-recombination region of hexaploid wheat. This work paves the way for the eventual isolation and characterization of the factor involved but also opens up possibilities to use this approach to precisely map other wheat genes located on proximal parts of wheat chromosomes that show highly reduced recombination.


Mapping wheat genes, in the centromeric and pericentromeric regions (~ 2/3rd of a given chromosome), poses a formidable challenge due to highly suppressed recombination. Using an example of compact spike locus (C-locus), this study provides an approach to precisely map wheat genes in the pericentromeric and centromeric regions that house ~ 30% of wheat genes. In club-wheat, spike compactness is controlled by the dominant C-locus, but previous efforts have failed to localize it, on a particular arm of chromosome 2D. We integrated radiation hybrid (RH) and high-resolution genetic mapping to locate C-locus on the short arm of chromosome 2D. Flanking markers of the C-locus span a physical distance of 11.0 Mb (231.0–242 Mb interval) and contain only 11 high-confidence annotated genes. This work demonstrates the value of this integrated strategy in mapping dominant genes in the low-recombination regions of the wheat genome. A comparison of the mapping resolutions of the RH and genetic maps using common anchored markers indicated that the RH map provides ~ 9 times better resolution that the genetic map even with much smaller population size. This study provides a broadly applicable approach to fine map wheat genes in regions of suppressed recombination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others


Download references


The authors thankfully acknowledge financial support from the United States Department of Agriculture—National Institute of Food and Agriculture (Award# 2020-67013-31460).

Author information

Authors and Affiliations



VT, ORL, and JL conceived the idea of the project and designed the experiments. AK, AS, KN, NR, PV, and RD conducted the experiments. VT, AK, ISY, and GC performed data analysis. BSG provided germplasm materials and inputs in discussion. BSG and NR provided resources for experiments. VT and AK wrote the manuscript with the inputs from the co-authors. All co-authors read and approved the manuscript.

Corresponding author

Correspondence to Vijay Tiwari.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest.

Data availability

All data supporting the conclusions of this article are provided within the article (and its Supplementary Information).

Additional information

Communicated by Peter Langridge.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kajla, A., Schoen, A., Paulson, C. et al. Physical mapping of the wheat genes in low-recombination regions: radiation hybrid mapping of the C-locus. Theor Appl Genet 136, 159 (2023).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: