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Genome-wise association study identified genomic regions associated with drought tolerance in mungbean (Vigna radiata (L.) R. Wilczek)

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Abstract

Key message

A total of 282 mungbean accessions were resequenced to identify genome-wide variants and construct a highly precise variant map, and drought tolerance-related loci and superior alleles were identified by GWAS.

Abstract

Mungbean (Vigna radiata (L.) R. Wilczek) is an important food legume crop that is highly adapted to drought environments, but severe drought significantly curtails mungbean production. Here, we resequenced 282 mungbean accessions to identify genome-wide variants and constructed a highly precise map of mungbean variants. A genome-wide association study was performed to identify genomic regions for 14 drought tolerance-related traits in plants grown under stress and well-watered conditions over three years. One hundred forty-six SNPs associated with drought tolerance were detected, and 26 candidate loci associated with more than two traits were subsequently selected. Two hundred fifteen candidate genes were identified at these loci, including eleven transcription factor genes, seven protein kinase genes and other protein coding genes that may respond to drought stress. Furthermore, we identified superior alleles that were associated with drought tolerance and positively selected during the breeding process. These results provide valuable genomic resources for molecular breeding and will accelerate future efforts aimed at mungbean improvement.

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Data availability

Data supporting the current study can be obtained by contacting the corresponding author (wujing@ caas.cn).

Abbreviations

QTL:

Quantitative trait locus

RIL:

Recombinant inbred line

GWAS:

Genome-wide association study

WGRS:

High-quality whole-genome resequencing

SNP:

Single-nucleotide polymorphism

InDeL:

Insertions or deletion

PCA:

Principal-component analyses

LD:

Linkage disequilibrium

BLUP:

Best linear unbiased predictions

DC:

Drought tolerance coefficient

DI:

Drought tolerance index

CV :

Coefficient of variation

HSW:

100-Seed weight

PLY:

Plot yield

PH:

Plant height

NB:

Number of branches

NMS:

Node number of the main stem

PNP:

Number of pods per plant

PL:

Pod length

NSP:

Number of seeds per pod

NS:

Number of seeds per plant

DGP:

Days of growth period

DTF:

Days to flowering

DTP:

Days to pod initiation

YP:

Yield per plant

BP:

Biomass per plant

MLM:

Mixed linear model

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Funding

This work was provided by National Key R&D Program of China (2019YFD1001300, 2019YFD1001305), China Agriculture Research System of MOF and MARA-Food Legumes (CARS-08), the Agricultural Science and Technology Innovation Program of CAAS.

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Authors and Affiliations

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yujie Chang, Shumin Wang, Lanfen Wang & Jing Wu

  2. Institute of Food Crop, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China

    Lin Peng & Liang Ji

Authors
  1. Yujie Chang
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  2. Lin Peng
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  4. Shumin Wang
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  5. Lanfen Wang
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  6. Jing Wu
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Contributions

LW and JW designed and supervised the research. LW, LP and LJ performed the phenotypic experiments. YC analysed data. YC wrote the paper. JW and SW reviewed the paper. All authors have read and approved the manuscript.

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Correspondence to Lanfen Wang or Jing Wu.

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Chang, Y., Peng, L., Ji, L. et al. Genome-wise association study identified genomic regions associated with drought tolerance in mungbean (Vigna radiata (L.) R. Wilczek). Theor Appl Genet 136, 40 (2023). https://doi.org/10.1007/s00122-023-04303-3

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