Abstract
Key message
Different digenomic Brassica autoallohexaploids were produced from the crosses of three allotetraploids and ancestral diploids and characterized for the cytological behavior of two subgenomes with two and four copies.
Abstract
Interspecific hybridization and allopolyploidization present an important pathway for plant evolution and breeding. In this study, different types of digenomic autoallohexaploids with two or four copies of two subgenomes (AAAACC, AACCCC, AAAABB, BBBBCC, BBCCCC) were synthesized by the crosses between three Brassica allotetraploids and their diploid progenitors and the chromosome doubling, and their meiotic behaviors were analyzed by fluorescence in situ hybridization (FISH). These autoallohexaploids showed some variations in pollen fertility and seed-sets and produced both euploid and aneuploid progenies with some chromosomes lost. Two subgenomes in these autoallohexaploids showed some aberrant pairings and segregations, and the degrees of meiotic regularity were negatively associated with the genome affinities. The chromosomes of the subgenome with four copies formed few quadrivalents with the average number < 2, and mainly paired as bivalents, and majority of the chromosomes from the subgenome with two copies gave the expected bivalents. The different extents of the equal and unequal segregations corresponded to the chromosome pairings. The development and cytological investigation of these autoallohexaploids provide not only the new germplasm for genetic research and breeding but also the new clues for the genome behavior and interplay between these subgenomes with different copies.
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Data availability
The hybridization and chromosome behavior data used to support the findings of this study are included within the article.
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Acknowledgements
We thank Dr. Jing Wang for her valuable suggestions in data analysis and contributions in laboratory management.
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This work was supported by Natural Science Foundation of China (Grant No. 31771827).
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XG: conceived and designed the experiments. BC, TW, FY and AH: performed the experiments. BC. and BZ. analyzed the data, BC., XG, WQ. and ZL. wrote the manuscript.
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Communicated by Annaliese S. Mason.
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Cai, B., Wang, T., Yue, F. et al. Production and cytology of Brassica autoallohexaploids with two and four copies of two subgenomes. Theor Appl Genet 135, 2641–2653 (2022). https://doi.org/10.1007/s00122-022-04136-6
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DOI: https://doi.org/10.1007/s00122-022-04136-6