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Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse

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Abstract

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A novel major QTL for FHB resistance was mapped to a 6.8 Mb region on chromosome 2D in a Chinese wheat cultivar Ji5265, and diagnostic KASP markers were developed for detecting it in a worldwide wheat collection.

Abstract

Fusarium head blight (FHB) is a serious disease in wheat (Triticum aestivum L.) and causes significant reductions in grain yield and quality worldwide. Breeding for FHB resistance is the most effective strategy to minimize the losses caused by FHB; therefore, identification of major quantitative trait loci (QTLs) conferring FHB resistance and development of diagnostic markers for the QTLs are prerequisites for marker-assisted selection (MAS). Ji5265 is a Chinese wheat cultivar resistant to FHB in multiple environments. An F6 population of 179 recombinant inbred lines (RILs) was developed from Ji5265 × Wheaton. The population was genotyped by genotyping-by-sequencing (GBS) and phenotyped for FHB Type II resistance in greenhouses. A major QTL, designated as QFhb-2DL, was mapped in a 6.8 Mb region between the markers GBS10238 and GBS12056 on the long arm of chromosome 2D in Ji5265 and explained ~ 30% of the phenotypic variation for FHB resistance. The effect of QFhb-2DL on FHB resistance was validated using near-isogenic lines (NILs) derived from residual heterozygotes from an F6 RIL of Ji5265 × Wheaton. The two flanking markers were converted into Kompetitive allele-specific PCR (KASP) markers (KASP10238 and KASP12056) and validated to be diagnostic in a collection of 2,065 wheat accessions. These results indicate that QFhb-2DL is a novel major QTL for resistance to FHB spread within a spike (Type II) and the two KASP markers can be used for MAS to improve wheat FHB resistance in wheat breeding programs.

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Acknowledgements

This is contribution number 22-160-J from the Kansas Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.

Funding

The funding was provided by Talent Funds of China Agricultural University (2020RC017), US Wheat and Barley Scab Initiative and USDA Agriculture and Food Research Initiative Competitive Grant (Grant No. 2022-68013-36439) (WheatCAP) from USDA National Institute of Food and Agriculture.

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  1. Hanwen Li and Fuping Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100083, China

    Hanwen Li, Fuping Zhang, Zhongfu Ni, Qixin Sun & Zhenqi Su

  2. College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Jixin Zhao

  3. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Jixin Zhao, Guihua Bai & Zhenqi Su

  4. USDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

    Guihua Bai, Paul St. Amand & Amy Bernardo

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  1. Hanwen Li
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Contributions

Bai GH and Su ZQ designed the research; Su ZQ, Li HW, Zhang FP, Zhao JX, and Bernardo A performed the research; Su ZQ, Li HW, Zhang FP, and St. Amand P analyzed the data; Li HW, Zhang FP, Su ZQ, Ni ZF, Sun QX, and Bai GH wrote the paper. All the authors read and approved the manuscript.

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Correspondence to Guihua Bai or Zhenqi Su.

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Fig. S1

Allele distribution of the parents and the RILs at KASP12056 marker locus showing clear separation among homozygous resistant and susceptible genotypes and heterozygotes. Blue and green dots represent homozygous resistant and homozygous susceptible genotypes, respectively, and cyan dots represent heterozygous genotypes. Black dots and crosses represent H2O controls and QFhb-2DL deletion lines, respectively.

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Li, H., Zhang, F., Zhao, J. et al. Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse. Theor Appl Genet 135, 1867–1877 (2022). https://doi.org/10.1007/s00122-022-04080-5

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