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Identification of genomic regions conferring rust resistance and enhanced mineral accumulation in a HarvestPlus Association Mapping Panel of wheat

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Abstract

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New genomic regions for high accumulation of 10 minerals were identified. The 1B:1R and 2NS translocations enhanced concentrations of four and two minerals, respectively, in addition to disease resistance.

Abstract

Puccinia species, the causal agents of rust diseases of wheat, have the potential to cause total crop failures due their high evolutionary ability to acquire virulence for resistance genes deployed in commercial cultivars. Hence, the discovery of genetically diverse sources of rust resistance is essential. On the other hand, biofortification of wheat for essential nutrients, such as zinc (Zn) and iron (Fe), is also an objective in wheat improvement programs to tackle micronutrient deficiency. The development of rust-resistant and nutrient-concentrated wheat cultivars would be important for sustainable production and the fight against malnutrition. The HarvestPlus association mapping panel (HPAMP) that included nutrient-dense sources from diverse genetic backgrounds was genotyped using a 90 K Infinium SNP array and 13 markers linked with rust resistance genes. The HPAMP was used for genome-wide association mapping to identify genomic regions underpinning rust resistance and mineral accumulation. Twelve QTL for rust resistance and 53 for concentrations of 10 minerals were identified. Comparison of results from this study with the published QTL information revealed the detection of already known and some putatively new genes/QTL underpinning stripe rust and leaf rust resistance in this panel. Thirty-six new QTL for mineral concentration were identified on 17 chromosomes. Accessions carrying the 1B:1R translocation accumulated higher concentrations of Zn, Fe, Copper (Cu) and sulphur (S). The 2NS segment showed enhanced accumulation of grain Fe and Cu. Fifteen rust-resistant and biofortified accessions were identified for use as donor sources in breeding programs.

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Acknowledgements

We acknowledge the CIMMYT scientists for constituting the HarvestPlus association mapping panel (HPAMP) and genotypic data. Acknowledgement is also due to Ms Annette Treadea, Research Assistant-Trial officer (Plant Breeding Institute, Narrabri) for her assistance in the field work and Mr Lyndon Palmer, Analytical Chemist (Flinders University Plant Nutrition, Flinders University) for estimating the mineral concentrations of wheat grain through Inductively Coupled Plasma Mass Spectrometry (ICPMS).

Funding

The first author thanks Australian Centre for International Agricultural Research (ACIAR) for the award of John Allwright Fellowship to pursue PhD study. We acknowledge the financial support received from Australian Research Council (ARC Grant: LP150101242) and Grains Research Development Corporation (GRDC) (9176057) Australia.

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Authors and Affiliations

  1. School of Life and Environmental Sciences, Faculty of Science, The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia

    Deepak Baranwal, Richard Trethowan, Harbans Bariana & Urmil Bansal

  2. College of Science & Engineering, Flinders University, Sturt Road, Bedford Park, South Australia, 5042, Australia

    Suong Cu & James Stangoulis

  3. Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, 813210, India

    Deepak Baranwal

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  1. Deepak Baranwal
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  2. Suong Cu
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Contributions

RT provided HPAMP; SC arranged the iselect 90 K SNP genotypic data; JS supervised mineral analysis; DB, UB and HB conducted rust screening; DB collected grain samples for mineral analysis, conducted genotyping of the panel with trait-linked markers, conducted statistical analysis and wrote the draft of this manuscript. SC, UB and HB supervised data analysis. UB provided overall supervision and edited the initial version of manuscript prior to circulation to all authors.

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Correspondence to Harbans Bariana or Urmil Bansal.

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Communicated by Lingrang Kong.

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Baranwal, D., Cu, S., Stangoulis, J. et al. Identification of genomic regions conferring rust resistance and enhanced mineral accumulation in a HarvestPlus Association Mapping Panel of wheat. Theor Appl Genet 135, 865–882 (2022). https://doi.org/10.1007/s00122-021-04003-w

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