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GmAOC4 modulates seed germination by regulating JA biosynthesis in soybean

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Abstract

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An allene oxide cyclase 4, GmAOC4 , was determined by GWAS and RT-PCR to be significantly associated with seed germination in soybean, and regulates seed germination by promoting more JA accumulation.

Abstract

The seed germination phase is a critical component of the plant lifecycle, and a better understanding of the mechanism behind seed germination in soybeans is needed. We used a genome-wide association study (GWAS) to detect a GWAS signal on chromosome 18. In this GWAS signal, SNP S18_56189166 was located within the 3’untranslated region of Glyma.18G280900, which encodes allene oxide cyclase 4 (named GmAOC4). Analysis of real-time PCR demonstrated that expression levels of GmAOC4 in the low-germination variety (KF, carrying SNP S18_56189166-T) were higher than in the high-germination variety (NN, carrying SNP S18_56189166-C). In these two varieties, KF showed a higher JA concentration than NN at 0 and 24 h after imbibition. Moreover, the overexpression of GmAOC4 led to an increase in the concentration of jasmonic acid (JA) in soybean hairy roots and Arabidopsis thaliana. Furthermore, it was found that GmAOC4-OE lines showed less seed germination than the wild type (WT) under normal conditions in Arabidopsis. After 7 days of ABA treatment, transgenic lines exhibited lower seed germination and higher expression levels of AtABI5 compared with WT, indicating that the overexpression of GmAOC4 resulted in hypersensitivity to ABA. Our findings demonstrate that GmAOC4, which promotes more JA accumulation, helps to regulate seed germination in soybeans.

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Acknowledgements

This work was supported by the Key R&D project of Jiangsu Province (BE2019376), the Natural Science Foundation of Jiangsu Province (BK20210154), the National Key Research and Development Program of China (2018YFE0112200), and Jiangsu Agriculture Science and Technology Innovation Fund[CX(20)2007].

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Authors and Affiliations

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Wei Zhang, Songsong Li, Hongmei Zhang, Xiaoqing Liu, Xiaoyan Cui, Li Song, Yuelin Zhu, Xin Chen & Huatao Chen

  2. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Wenjing Xu, Songsong Li & Yuelin Zhu

  3. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China

    Li Song

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  1. Wei Zhang
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  2. Wenjing Xu
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  3. Songsong Li
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  4. Hongmei Zhang
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  5. Xiaoqing Liu
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  6. Xiaoyan Cui
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  7. Li Song
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  8. Yuelin Zhu
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  9. Xin Chen
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  10. Huatao Chen
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Contributions

ZW, WX, HZ, and SL were all involved in the experimental design and the collection of phenotypic data; ZW and XC conducted the phenotypic analysis; WX, LS, XL, YZ, and CX helped revise the manuscript; ZW assessed the experimental results; ZW and HT wrote the paper. The manuscript was reviewed by all authors, who each contributed suggestions.

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Correspondence to Xin Chen or Huatao Chen.

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Communicated by Istvan Rajcan.

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Zhang, W., Xu, W., Li, S. et al. GmAOC4 modulates seed germination by regulating JA biosynthesis in soybean. Theor Appl Genet 135, 439–447 (2022). https://doi.org/10.1007/s00122-021-03974-0

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